LOCUS       sequence-45410-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     Lees Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lox-Stop-Lox TOPO Hygro
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..94
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      125..134
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    159..280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(170..189)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     224..243
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(705..725)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attaaccacg cgtggcgcgc cttgagctcg gatccgggcc ccccctcgat cgaggtcgac
       61 ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg cagacttact
      121 caagccatgg tggctagctt gcggaattcc agacatgata agatacattg atgagtttgg
      181 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      241 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      301 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      361 caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta
      421 ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata
      481 gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta
      541 tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct
      601 ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga
      661 ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt
      721 tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct
      781 tctgagcaaa acaggttttc ctcattaaag g
//
