LOCUS       sequence-454116-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Montipora efflorescens GFP-like chromoprotein under T7 
            with a 6xHIS tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Beckham J
  TITLE     Josh Beckham Lab plasmids 2024
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGBR22-pGEM-T
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..663
                     /codon_start=1
                     /product="dimeric Keima-Red fluorescent protein"
                     /label=dKeima-Red
                     /translation="MSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPLSQYGSIPFTKYPEDIPDYVKQSFPEGYTWERIMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGVNFPPNGPVMQKKTQGWEPNTERLFARDGMLIGNNFMALKL
                     EGGGYYLCEFKSTYKAKKPVRMPGYHYVDRKLDVTSHNKDYTFVEQCEISIARHSLLG"
     CDS             664..681
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagtgtga tcgctaaaca aatgacctac aaggtttata tgtcaggcac ggtcaatgga
       61 cactactttg aggtcgaagg cgatggaaaa ggaaagcctt acgaggggga gcagacggta
      121 aagctcactg tcaccaaggg tggacctctg ccatttgctt gggatatttt atcaccactg
      181 tctcaatacg gaagcatacc attcaccaag taccctgaag acatccctga ttatgtaaag
      241 cagtcattcc ctgagggata tacatgggag aggatcatga actttgaaga tggtgcagtg
      301 tgtactgtca gcaatgattc cagcatccaa ggcaactgtt tcatctacaa tgtcaaaatc
      361 tctggtgtga actttcctcc caatggacct gttatgcaga agaagacaca gggctgggaa
      421 cccaacactg agcgtctctt tgcacgagat ggaatgctga taggaaacaa ctttatggct
      481 ctgaagttgg aaggaggtgg ttactatttg tgtgaattca aatctactta caaggcaaag
      541 aagcctgtga ggatgccagg gtatcactat gttgaccgca aactggatgt aaccagtcac
      601 aacaaggatt acacatttgt tgagcagtgt gaaatatcca ttgcacgcca ctctttgctc
      661 ggtcatcacc atcaccatca ctaa
//
