LOCUS       sequence-454123-       16089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 plasmid with Aspergillus optimized Mad7 and argB selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Vanegas KG, Rendsvig JKH, Jarczynska ZD, Cortes MVCB, van Esch AP, 
            Morera-Gomez M, Contesini FJ, Mortensen UH
  TITLE     A Mad7 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   J Fungi (Basel). 2022 Dec 22;9(1):16. doi: 10.3390/jof9010016.
  PUBMED    36675838
REFERENCE   2  (bases 1 to 16089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2022-12-22"; volume: "9(1):16. doi"; pages: " 
            10.3390/jof9010016"
COMMENT     Sequence Label: pDIV299
FEATURES             Location/Qualifiers
     source          1..16089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6838
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             11818..11838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(14589..14622)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(15092..15108)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15207..15224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15378..15966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15458..15477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16088..16089,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgtgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca tggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat
     1981 aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag
     2041 tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct
     2101 gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga
     2161 ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt
     2221 attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc
     2281 tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac
     2341 cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt
     2401 ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat
     2461 tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca
     2521 ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc
     2581 tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct
     2641 gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt
     2701 gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc
     2761 ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta
     2821 cggggctgaa tgtaacttgc cyttcctttc tcatcgcgcg gcaagacagg cttgctcaaa
     2881 ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag
     2941 ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat
     3001 ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat
     3061 gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct
     3121 gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgckct
     3181 tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgctr
     3241 cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat
     3301 cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt
     3361 gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa
     3421 taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact
     3481 cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc
     3541 agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc
     3601 tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta
     3661 tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga
     3721 ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct
     3781 atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg
     3841 actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg
     3901 gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg
     3961 ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc
     4021 gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac
     4081 aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca
     4141 gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg
     4201 ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc
     4261 caaacataca cgatttggag agggcctagg tataagagtt tttgagtaga acgcatgtga
     4321 gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc
     4381 aatggaaacg gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc
     4441 taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt
     4501 acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
     4561 tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga
     4621 tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc
     4681 actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc
     4741 aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca
     4801 ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg
     4861 acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga
     4921 tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct
     4981 actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga
     5041 aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac
     5101 aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac
     5161 ttggtgacta gtccctgact agtcaagyag catggtatga aggcttgttt gaaggcactg
     5221 ttctcccaac cccgactcct accaagaagm gcaagcgagc tgcgtaagtt tctgtgagtc
     5281 taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt
     5341 gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac
     5401 tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac
     5461 agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc
     5521 cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa
     5581 aggaarggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag
     5641 ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa
     5701 ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta
     5761 gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt
     5821 ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc
     5881 agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc
     5941 ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt
     6001 cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag
     6061 aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt
     6121 aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca
     6181 gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc
     6241 aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg
     6301 ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt
     6361 agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc
     6421 aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag
     6481 tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc
     6541 cccttmtagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta
     6601 gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa
     6661 cgtttatacg tcaaaagcat ttataagctt ttaccctaaa gtagcttgct tgtgtgttta
     6721 gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt
     6781 aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctta
     6841 acaatctatt ctccgcttgg tgatgctaaa gggctttcaa tagaccttgt aagtgaagga
     6901 gatggagccg tcaatccgct accctgcctc tggtcagttg gtctcagcaa tgtaccctgt
     6961 gagtcttgat agactagttg gtgactagtc tctgtagatg gaacaaatgg tgcttgagag
     7021 ggcacgtatg gcagagctcc agtcatctgc ggaacatata ctgggcccgg gaagatctca
     7081 tggtcatagc tgtttccgct gagggtttaa tgctgagggt ttaattaaga cctcagccga
     7141 gacagcagaa tcaccgccca agttaagcct ttgtgctgat catgctctcg aacgggccaa
     7201 gttcgggaaa agcaaaggag cgtttagtga ggggcaattt gactcacctc ccaggcaaca
     7261 gatgaggggg gcaaaaagaa agaaattttc gtgagtcaat atggattccg agcatcattt
     7321 tcttgcggtc tatcttgcta cgtatgttga tcttgacgct gtggatcaag caacgccact
     7381 cgctcgctcc atcgcaggct ggtcgcagac aaattaaaag gcggcaaact cgtacagccg
     7441 cggggttgtc cgctgcaaag tacagagtga taaaagccgc catgcgacca tcaacgcgtt
     7501 gatgcccagc tttttcgatc cgagaatcca ccgtagaggc gatagcaagt aaagaaaagc
     7561 taaacaaaaa aaaatttctg cccctaagcc atgaaaacga gatggggtgg agcagaacca
     7621 aggaaagagt cgcgctgggc tgccgttccg gaaggtgttg taaaggctcg acgcccaagg
     7681 tgggagtcta ggagaagaat ttgcatcggg agtggggcgg gttacccctc catatccaat
     7741 gacagatatc taccagccaa gggtttgagc ccgcccgctt agtcgtcgtc ctcgcttgcc
     7801 cctccataaa aggatttccc ctccccctcc cacaaaattt tctttccctt cctctccttg
     7861 tccgcttcag tacgtatatc ttcccttccc tcgcttctct cctccatcct tctttcatcc
     7921 atctcctgct aacttctctg ctcagcacct ctacgcatta ctagccgtag tatctgagca
     7981 cttctccctt ttatattcca caaaacataa cacaaccttc accatatgaa caacggcacc
     8041 aacaacttcc agaacttcat cggcatctcc agcctccaaa agaccctccg caacgctctc
     8101 atccccaccg agactaccca gcagttcatc gtcaagaacg gcatcatcaa agaggatgag
     8161 ctgcgcggcg agaaccgcca gatcctcaag gatatcatgg atgattacta ccgcggcttt
     8221 atctccgaga ctctctcctc catcgatgat atcgattgga ccagcctctt cgagaagatg
     8281 gaaatccagc tgaagaacgg cgataacaag gataccctga tcaaagagca gaccgagtac
     8341 cgcaaggcca tccacaagaa gttcgccaac gacgatcgct tcaagaatat gttctccgcc
     8401 aagctcatct ccgatatcct gcctgagttc gtcatccaca acaacaacta ctccgcctcc
     8461 gagaaagagg aaaagaccca ggtcatcaag ctgttctccc gcttcgccac ctccttcaag
     8521 gattacttca agaaccgcgc caactgcttc tccgccgacg atatctcctc cagctcctgc
     8581 caccgcatcg tcaacgataa cgccgagatc ttcttctcca acgctctggt ctaccgccgc
     8641 atcgtgaagt ccctctccaa cgatgacatc aacaagatct ccggcgatat gaaggattcc
     8701 ctcaaagaga tgtccctcga ggaaatctac tcctacgaga agtacggcga gttcattacc
     8761 caagagggca tctccttcta caacgatatc tgcggcaagg tcaactcctt catgaacctc
     8821 tactgccaga agaacaaaga gaacaagaac ctgtacaagc tccagaagct ccacaagcag
     8881 atcctctgta tcgccgatac cagctacgag gtcccctaca agttcgagtc cgatgaggaa
     8941 gtctaccagt ccgtcaacgg cttcctcgat aacatcagct ccaagcacat cgtcgagcgc
     9001 ctccgcaaga tcggcgacaa ctacaacggc tacaacctcg ataagatcta catcgtgtcc
     9061 aagttctacg agtctgtctc gcaaaagacc taccgcgatt gggaaaccat caacaccgct
     9121 ctcgagatcc attacaacaa catcttgccc ggcaacggca agtccaaggc cgataaggtc
     9181 aagaaggccg ttaagaacga tctgcagaag tccatcaccg agatcaacga gctggtgtcc
     9241 aactacaagc tctgctccga tgacaacatc aaggccgaaa cctacatcca cgagatctcc
     9301 cacatcctga acaacttcga ggcccaagag ctgaagtaca accccgagat ccacctcgtc
     9361 gagagcgagc tgaaggcctc cgagttgaag aacgtcctcg atgtcatcat gaacgccttc
     9421 cactggtgct ccgtgttcat gaccgaggaa ctcgtcgata aggataacaa cttctacgcc
     9481 gagctggaag aaatctacga tgagatctac cccgtcatct ccctgtacaa cctggtccgc
     9541 aactacgtca cccagaagcc ttactccacc aagaagatca agctcaactt cggcatcccc
     9601 actctcgccg atggttggtc caagtccaaa gagtactcca acaacgccat catcctcatg
     9661 cgcgataacc tgtactacct cggcatcttc aacgccaaga acaagcccga taagaagatt
     9721 atcgagggca acaccagcga gaacaagggc gattacaaga agatgatcta caacctgctg
     9781 cctggtccta acaagatgat ccccaaggtg ttcctgtcct ccaagaccgg cgtcgagact
     9841 tacaagccct ccgcttacat cctcgaaggc tacaagcaga acaagcacat taagtccagc
     9901 aaggatttcg atatcacctt ctgccacgat ctgatcgact atttcaagaa ctgcattgcc
     9961 atccatcctg agtggaagaa cttcggcttc gatttctccg atacctccac ctacgaggat
    10021 atctccggct tctaccgcga ggtcgagctg cagggctaca agatcgactg gacctacatc
    10081 agcgagaagg atatcgatct cctgcaagag aagggccagc tctacctctt ccagatctat
    10141 aacaaagatt tctccaagaa gtccaccggc aacgacaacc tccacaccat gtacctcaag
    10201 aacctcttca gcgaggaaaa cctcaaggac atcgtcctca agctgaacgg cgaggctgaa
    10261 atcttcttca gaaagtcctc catcaagaac cccatcatcc ataagaaggg ctccatcctc
    10321 gtcaaccgca cctacgaagc cgaggaaaag gatcagttcg gcaacatcca gatcgtccgc
    10381 aagaacatcc ccgagaacat ctaccaagag ttgtacaagt acttcaacga caagtccgac
    10441 aaagagctgt ccgacgaggc cgcaaagctc aagaacgtcg tcggccacca tgaggccgcc
    10501 accaacatcg ttaaggatta ccgctatacc tacgacaagt atttcctgca catgcccatc
    10561 accatcaact tcaaggccaa caagaccggt ttcatcaacg atcgcatcct ccagtatatc
    10621 gccaaagaga aggatctcca cgtcatcggt atcgatcgcg gcgagcgcaa cctcatctac
    10681 gtgtccgtca tcgatacctg cggtaacatc gtggaacaga agtccttcaa catcgtcaat
    10741 ggctacgatt accagatcaa gttgaagcag caagagggcg ctcgccagat cgctcgcaaa
    10801 gagtggaaag agatcggcaa gatcaaagaa atcaaagagg gctacctgag cctggtgatc
    10861 catgagattt ccaagatggt gatcaagtac aacgctatca ttgccatgga agatctgtcc
    10921 tacggtttca agaagggccg cttcaaggtc gaacgccagg tctaccaaaa gttcgaaacc
    10981 atgctgatca acaagttgaa ctacctcgtc tttaaggaca tctccattac cgagaacggc
    11041 ggcctcctca agggctacca gctcacctac attcccgata agctgaaaaa cgtcggccat
    11101 cagtgcggct gcatcttcta cgtccctgcc gcctacacct ccaagatcga tcccaccacc
    11161 ggcttcgtca acatcttcaa gttcaaggat ctcaccgtcg atgccaagcg cgaattcatc
    11221 aagaagtttg attccatccg ctacgattcc gagaagaatc tcttctgttt caccttcgac
    11281 tacaacaatt tcatcactca gaacaccgtc atgtccaagt cgtcctggtc cgtctacacc
    11341 tacggcgtcc gcatcaagcg ccgcttcgtt aacggccgct tctcgaacga gtccgacacc
    11401 atcgacatca ccaaggatat ggaaaagaca ctcgagatga ccgatatcaa ctggcgcgac
    11461 ggccacgacc tccgccagga catcatcgac tacgagatcg tccagcatat cttcgaaatc
    11521 ttccgcctca ccgtccagat gcgcaactcc ctgtccgagt tggaggatcg cgactacgat
    11581 cgcctcatct cccctgtcct caacgagaac aacattttct acgatagcgc caaggccggc
    11641 gacgccctgc ctaaggatgc cgacgccaac ggcgcctact gtatcgctct caagggcctg
    11701 tacgagatca agcaaatcac cgaaaactgg aaagaggacg gaaagttcag ccgcgacaag
    11761 ctgaagatct ccaacaagga ttggttcgat ttcatccaaa acaagcgcta cctgcctccc
    11821 aagaagaagc gcaaggtctg aagcggacat tcgatttatg ccgttatgac ttccttaaaa
    11881 aagcctttac gaatgaaaga aatggaatta gacttgttat gtagttgatt ctacaatgga
    11941 ttatgattcc tgaacttcaa atccgctgtt cattattaat ctcagctctt cccgtaaagc
    12001 caatgttgaa actattcgta aatgtacctc gttttgcgtg taccttgctt atcacgtgat
    12061 attacatgac ctggacagag ttctgcgcga aagtcataac gtaaatcccg ggcggtaggt
    12121 gcgtcccggg cggaaggtag ttttctcgtc caccccaacg cgtttatcaa cctcaacttt
    12181 caacaaccat catgccacca aaagcgcgta aaacaaagcg agatttgatt gagcaagagg
    12241 gcaggatcca atgcgcgatt caagacatta aaaatggaaa atttcaaaaa attgcgcccg
    12301 cagcgcgtgc atacaaaatt catcccaata cattaagacc tcagcactag gtaatatcgc
    12361 gtgcattccg cggttttttg gggtagtcat ctaatgaaac agacccggac gcagcagagg
    12421 aagcccgcga tgactctata ccaccgtacg ccgatatatc atcatcgcgg cgatggagaa
    12481 gtggggttga ctccgaagac acttcaaagg agcgacgctg ttgatttgta gacgacgctt
    12541 gatagggaga agcattattg tcgtgatgct cgcccaacag aggccgactc gcctcatccg
    12601 tcataacgaa cgctgtgtaa agcggagtgg gggggaaagt gtggattgtg gagagtatgc
    12661 gatagtgttg aggctgatca gacggcgaat cgggccagat atgaccagtt tagaggcctc
    12721 atttgactat aatttacata aattagataa atagagatga acgcatgcaa taattgcagc
    12781 aaatattgat gaagcgagag gtaggacgat gaaggactgt gagcagttca aggtatcagc
    12841 agagtcaagg gcctgatgca atggcggtga tccgtgatca gcgaacggaa ggggcgctaa
    12901 ctctgtttct ttaccaatga tcggaagctc ctgctggcgg acttatgagt cattcacgaa
    12961 tcatttctca gttatttgtg gatgccctcg ttctgtccac aatttctttc cgccccaagt
    13021 cttttaagtt ctttaacatc tatattcttg cacttccaat ggcatccctt cgctccgtac
    13081 tcaagagcca gagcttgcga cacaccgtgc gatcctactc ctcgcaaacc atgcctcccg
    13141 cctcaccctt tgctccccgc cacttcctct ccattgcgga cctctcgccc tccgagttcg
    13201 caacccttgt tcgcaatgcc tcctcacaca aacgggctat caagtcgggg tcaatgcccc
    13261 aaaacttgca gggatcactc cttgggaaaa ctgtggccat gatcttcagc aaacgaagca
    13321 cgaggacaag ggtatctaca gaaggggccg ttgtgcagat gggaggtcat ccgatgttct
    13381 tgggcaagga tgatatccaa ctaggtgtca acgagtccct atacgacacc tccgttgtca
    13441 tttcgtccat ggtatcctgc attgtagccc gtgtcggtaa acatgcagag gtcgcagatc
    13501 tggcgaagca ctcttcggtt ccagtcatca atgctttgtg tgactctttc caccctctcc
    13561 aagccgtggc cgatttccag accatctatg aagcattcac ccccaaggcg caccaccttt
    13621 caagtctagg gttggaagga ttgaagatcg cttgggtggg tgacgccaac aacgtcctgt
    13681 tcgatatggc cattgctgct acaaaaatgg gtgtcgacat tgctgtcgct actcccaagg
    13741 ggtacgaaat ccctcctcac atgctggagc tcatcaagtc tgctggagag ggtgtctcga
    13801 aaccaggaaa gcttctgcaa accaatattc ccgaagaagc ggtcaaggac gccgatattc
    13861 tggtcacaga cacctgggtc tctatgggcc aagaggaaga gaaggctcag aggctgaagg
    13921 agtttgatgg tttccaaatc actgctgaac tcgccaagcg aggaggagct aaggagggct
    13981 ggaagttcat gcactgtctc ccgcgacacc ctgaggaggt cagcgacgag gttttctaca
    14041 gcaaccggtc acttgtcttc cctgaggctg agaaccggtt atgggctgcg atttccgcct
    14101 tggagggttt cgttgtcaat aagggaaaaa ttgaataaat ataaccaggc ttccatttaa
    14161 atatatagag gctggcgtta tcaaactgat gagttgacgg gtatgagatc attccgtccc
    14221 taaatatatt tactccgatc acgtaaaagc ctgttagtag aagcattttc ccaattatcc
    14281 tgaccaattc tttctagcat atatcaaata actaattgac atgttctctc gcttccttat
    14341 attactcaga gtattggaaa tggggcaaat cgcacccggt gactttcaca tgtcacgaat
    14401 gcggagtcgt cctagccaag gtagatccag gcctaacaca ccccaaccct cgactctcct
    14461 cattccatac tcttgacctc tatccagcac attcttctga ggtttcgcaa tggctgtagg
    14521 tggcgctgag gcatccccac taccgcatta agacctcagc gcggccgcaa atttaaataa
    14581 aatgaagtga agttcctata ctttctagag aataggaact tctatagtga gtcgaataag
    14641 ggcgacacaa aatttattct aaatgcataa taaatactga taacatctta tagtttgtat
    14701 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta
    14761 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
    14821 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
    14881 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
    14941 tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat gctctttccc taaactcccc
    15001 ccataaaaaa acccgccgaa gcgggttttt acgttatttg cggattaacg attactcgtt
    15061 atcagaaccg cccagggggc ccgagcttaa gactggccgt cgttttacaa cacagaaaga
    15121 gtttgtagaa acgcaaaaag gccatccgtc aggggccttc tgcttagttt gatgcctggc
    15181 agttccctac tctcgccttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    15241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggt
    15301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    15361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    15421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    15481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    15541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    15601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gccctaccgc
    15661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    15721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    15781 ttcttgaagt ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    15841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    15901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    15961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc
    16021 gtaactcacg ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat
    16081 gctctgctt
//
