LOCUS       sequence-454125-       15373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) 
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15373)
  AUTHORS   Vanegas KG, Rendsvig JKH, Jarczynska ZD, Cortes MVCB, van Esch AP, 
            Morera-Gomez M, Contesini FJ, Mortensen UH
  TITLE     A Mad7 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   J Fungi (Basel). 2022 Dec 22;9(1):16. doi: 10.3390/jof9010016.
  PUBMED    36675838
REFERENCE   2  (bases 1 to 15373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2022-12-22"; volume: "9(1):16. doi"; pages: " 
            10.3390/jof9010016"
COMMENT     Sequence Label: pDIV301
FEATURES             Location/Qualifiers
     source          1..15373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(63..96)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(681..698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(852..1440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(932..951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1562..2353,2354..2422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2185..2204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2423..2514)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2556..2575
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2581..2600
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2807..2840
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      3153..8401
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             13381..13401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14233..14607
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 aggtaaatcc ccactaccgc attaagacct cagcgcggcc gcaaatttaa ataaaatgaa
       61 gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa taagggcgac
      121 acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt
      181 ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt
      241 tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc
      301 ataaataata gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct
      361 tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc
      421 aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa
      481 aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga
      541 accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt
      601 agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc
      661 ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      721 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      841 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      901 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      961 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1021 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1081 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1141 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1201 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1261 aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1321 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1381 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1441 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact
     1501 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg
     1561 cttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1681 ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa
     1741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1861 acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2041 tcatggttat ggcagcgcta cataattctc ttactgtcat gccatccgta agatgctttt
     2101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2401 cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc atttatcagg
     2461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2521 tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt atacctgaat
     2581 atggctcata acaccccttg tttgcctggc ggcagtagcg cggtggtccc acctgacccc
     2641 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggac tccccatgcg
     2701 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2761 tcgcccgggc taattatggg gtgtcgccct tattcgactc tatagtgaag ttcctattct
     2821 ctagaaagta taggaacttc tgaagtgggg atttaaatgc ggccgcgctg agggtttaat
     2881 cgacgaagca gctgacggcc agtgccaagc ttaacgcgta ccgggcccag tatatgttcc
     2941 gcagatgact ggagctctgc catacgtgcc ctctcaagca ccatttgttc catctacaga
     3001 gactagtcac caactagtct atcaagactc acagggtaca ttgctgagac caactgacca
     3061 gaggcagggt agcggattga cggctccatc tccttcactt acaaggtcta ttgaaagccc
     3121 tttagcatca ccaagcggag aatagattgt taagcttatt ttttgtatac tgttttgtga
     3181 tagcacgaag tttttccacg gtatcttgtt aaaaatatat atttgtggcg ggcttaccta
     3241 catcaaatta ataagagact aattataaac taaacacaca agcaagctac tttagggtaa
     3301 aagtttataa atgcttttga cgtataaacg ttgcttgtat ttattattac aattaaaggt
     3361 ggatagaaaa cctagagact agttagaaac taatctcagg tttgcgttaa actaaatcag
     3421 agcccgagag gttaacagaa cctagaaggg gactagatat ccgggtaggg aaacaaaaaa
     3481 aaaaaacaag acatccacat attagggaga ctagttagaa gctagttcca ggactaggaa
     3541 aataaaagac aatgatacca cagtctagtt gacaactaga tagattctag attgaggcca
     3601 aagtctctga gatccaggtt agttgcaact aatactagtt agtatctagt ctcctataac
     3661 tctgaagcta gaataactta ctactattat cctcaccact gttcagctgc gcaaacggag
     3721 tgattgcaag gtgttcagag actagttatt gactagtcag tgactagcaa taactaacaa
     3781 ggtattaacc taccatgtct gccatcaccc tgcacttcct cgggctcagc agccttttcc
     3841 tcctcatttt catgctcatt ttccttgttt aagactgtga ctagtcaaag actagtccag
     3901 aaccacaaag gagaaatgtc ttaccacttt cttcattgct tgtctctttt gcattatcca
     3961 tgtctgcaac tagttagagt ctagttagtg actagtccga cgaggacttg cttgtctccg
     4021 gattgttgga ggaactctcc agggcctcaa gatccacaac agagccttct agaagactgg
     4081 tcaataacta gttggtcttt gtctgagtct gacttacgag gttgcatact cgctcccttt
     4141 gcctcgtcaa tcgatgagaa aaagcgccaa aactcgcaat atggctttga accacacggt
     4201 gctgagacta gttagaatct agtcccaaac tagcttggat agcttacctt tgycctttgc
     4261 gttgcgacag gtcttgcagg gtatggttcc tttctcacca gctgatttag ctgccttgct
     4321 accctcacgg cggatctgca taaagagtgg ctagaggtta taaattagca ctgatcctag
     4381 gtacggggct gaatgtaact tgccyttcct ttctcatcgc gcggcaagac aggcttgctc
     4441 aaattcctac cagtcacagg ggtatgcacg gcgtacggac cacttgaact agtcacagat
     4501 tagttagcaa ctagtctgca ttgaatggct gtacttacgg gccctcgcca ttgtcctgat
     4561 catttccagc ttcaccctcg ttgctgcaaa gtagttagtg actagtcaag gactagttga
     4621 aatgggagaa gaaactcacg aattctcgac acccttagta ttgtggtcct tggacttggt
     4681 gctgctatat attagctaat acactagtta gactcacaga aacttacgca gctcgcttgc
     4741 gcttcttggt aggagtcggg gttgggagaa cagtgccttc aaacaagcct tcataccatg
     4801 ctacttgact agtcagggac tagtcaccaa gtaatctaga taggacttgc ctttggcctc
     4861 catcagttcc ttcatagtgg gaggtccatt gtgcaatgta aactccatgc cgtgggagtt
     4921 cttgtccttc aagtgcttga ccaatatgtt tctgttggca gagggaacct gtcaactagt
     4981 taataactag tcagaaacta gtatagcagt agactcactg tacgcttgag gcatcccttc
     5041 actcggcagt agacttcata tggatggata tcaggcacgc cattgtcgtc ctgtggacta
     5101 gtcagtaact aggcttaaag ctagtcgggt cggcttacta tcttgaaatc cggcagcgta
     5161 agctccccgt ccttaactgc ctcgagatag tgacagtact ctggggactt tcggagatcg
     5221 ttatcgcgaa tgctcggcat actaatcgtt gactagtctt ggactagtcc cgagcaaaaa
     5281 ggattggagg agaaggagga aggtgagagt gagacaaaga gcgaaataag agcttcaaag
     5341 gctatctcta agcagtatga aggttaagta tctagttctt gactagattt aaaagagatt
     5401 tcgactagtt atgtacctgg agtttggata taggaatgtg ttgtggtaac gaaatgtaag
     5461 ggggaggaaa gaaaaagtcg gtcaataggt aactctaagt cggccattcc tttttgggag
     5521 tcgctaacca taaacggcat ggtcgactta gagttagctc agggaattta gggagttatc
     5581 tgcgaccacc gaggaacggc ggaatgccaa agaatcccga tggagctcta gctggcggtt
     5641 gacaacccca ccttttggcg tttctgcggc gttgcaggcg ggactggata cttcgtagaa
     5701 ccagaaaggc aaggcagaac gcgctcagca agagtgttgg aagtgatagc atgatgtgcc
     5761 ttgttaacta ggtcaaaatc tgcagtatgc ttgatgttat ccaaagtgtg agagaggaag
     5821 gtccaaacat acacgattgg gagagggcct aggtataaga gtttttgagt agaacgcatg
     5881 tgagcccagc catctcgagg agattaaaca cgggccggca tttgatggct atgttagtac
     5941 cccaatggaa acggtgagag tccagtggtc gcagataact ccctaaattc cctgagctaa
     6001 ctctaagtcg accatgccgt ttatggttag cgcctcccaa aaaggaatgg ccgacttaga
     6061 gttacctctt gaccgacttt ttctttcctc ccccttacat ttcgttacca caacacattc
     6121 ctatatccaa actccaggta cataactagt cgaaatctct tttaaatcta gtcaagaact
     6181 agatacttaa ccttcatact gcttagagat agcctttgaa gctcttattt cgctctttgt
     6241 ctcactctca ccttcctcct cctcctccaa tcctttttgc tcgggactag tccaagacta
     6301 gtcaacgatt agtatgccga gcattcgcga taacgatctc cgaaagtccc cagagtactg
     6361 tcactatctc gaggcagtta aggacgggga gcttacgctg ccggatttca agatagtaag
     6421 ccgacccgac tagctttaag cctagttact gactagtcca caggacgaca atggcgtgcc
     6481 tgatatccat ccatatgaag tctactgccg agtgaaggga tgcctcaagc gtacagtgag
     6541 tctactgcta tactagtttc tgactagtta ttaactagtt gacaggttcc ctctgccaac
     6601 agaaacatat tggtcaagca cttgaaggac aagaactccc acggcatgga gtttacattg
     6661 cacaatggac ctcccactat gaaggaactg atggaggcca aaggcaagtc ctatctagat
     6721 tacttggtga ctagtccctg actagtcaag tagcatggta tgaaggcttg tttgaaggca
     6781 ctgttctccc aaccccgact cctaccaaga agcgcaagcg agctgcgtaa gtttctgtga
     6841 gtctaactag tgtattagct aatatatagc agcaccaagt ccaaggacca caatactaag
     6901 ggtgtcgaga attcgtgagt ttcttctccc atttcaacta gtccttgact agtcactaac
     6961 tactttgcag caacgagggt gaagctggaa atgatcagga caatggcgag ggcccgtaag
     7021 tacagccatt caatgcagac tagttgctaa ctaatctgtg actagttcaa gtggtccgta
     7081 cgccgtgcat acccctgtga ctggtaggaa tttgagcaag cctgtcttgc cgcgcgatga
     7141 gaaaggaarg gcaagttaca ttcagccccg tacctaggat cagtgctaat ttataacctc
     7201 tagccactct ttatgcagat ccgccgtgag ggtagcaagg cagctaaatc agctggtgag
     7261 aaaggaacca taccctgcaa gacctgtcgc aacgcaaagg rcaaaggtaa gctatccaag
     7321 ctagtttggg actagattct aactagtctc agcaccgtgt ggttcaaagc catattgcga
     7381 gttttggcgc tttttctcat cgattgacga ggcaaaggga gcgagtatgc aacctcgtaa
     7441 gtcagactca gacaaagacc aactagttat tgaccagtct tctagaaggc tctgttgtgg
     7501 atcttgaggc cctggagagt tcctccaaca atccggagac aagcaagtcc tcgtcggact
     7561 agtcactaac tagactctaa ctagttgcag acatggataa tgcaaaagag acaagcaatg
     7621 aagaaagtgg taagacattt ctcctttgtg gttctggact agtctttgac tagtcacagt
     7681 cttaaacaag gaaaatgagc atgaaaatga ggaggaaaag gctgctgagc ccgaggaagt
     7741 gcagggtgat ggcagacatg gtaggttaat accttgttag ttattgctag tcactgacta
     7801 gtcaataact agtctctgaa caccttgcaa tcactccgtt tgcgcagctg aacagtggtg
     7861 aggataatag tagtaagtta ttctagcttc agagttatag gagactagat actaactagt
     7921 attagttgca actaacctgg atctcagaga ctttggcctc aatctagaat ctatctagtt
     7981 gtcaactaga ctgtggtatc attgtctttt attttcctag tcctggaact agcttctaac
     8041 tagtctccct aatatgtggm tgtcttgttt tttttttttg tttccctacc cggatatcta
     8101 gtccccttct aggttctgtt aacctctcgg gctctgattt agtttaacgc aaacctgaga
     8161 ttagtttcta actagtctct aggttttcta tccaccttta attgtaataa taaatacaag
     8221 caacgtttat acgtcaaaag catttataaa cttttaccct aaagtagctt gcttgtgtat
     8281 ttagtttata attagtctct tattaatttg atgtaggtaa gcccgccaca aatatatatt
     8341 tttaacaaga taccgtggaa aaacttcgtg ctatcacaaa acagtataca aaaaataagc
     8401 ttaacaatct attctccgct tggtgatgct aaagggcttt caatagacct tgtaagtgaa
     8461 ggagatggag ccgtcaatcc gctaccctgc ctctggtcag ttggtctcag caatgtaccc
     8521 tgtgagtctt gatagactag ttggtgacta gtctctgtag atggaacaaa tggtgcttga
     8581 gagggcacgt atggcagagc tccagtcatc tgcggaacat atactgggcc cgggaagatc
     8641 tcatggtcat agctgtttcc gctgagggtt taatgctgag ggtttaatta agacctcagc
     8701 cgagacagca gaatcaccgc ccaagttaag cctttgtgct gatcatgctc tcgaacgggc
     8761 caagttcggg aaaagcaaag gagcgtttag tgaggggcaa tttgactcac ctcccaggca
     8821 acagatgagg ggggcaaaaa gaaagaaatt ttcgtgagtc aatatggatt ccgagcatca
     8881 ttttcttgcg gtctatcttg ctacgtatgt tgatcttgac gctgtggatc aagcaacgcc
     8941 actcgctcgc tccatcgcag gctggtcgca gacaaattaa aaggcggcaa actcgtacag
     9001 ccgcggggtt gtccgctgca aagtacagag tgataaaagc cgccatgcga ccatcaacgc
     9061 gttgatgccc agctttttcg atccgagaat ccaccgtaga ggcgatagca agtaaagaaa
     9121 agctaaacaa aaaaaaattt ctgcccctaa gccatgaaaa cgagatgggg tggagcagaa
     9181 ccaaggaaag agtcgcgctg ggctgccgtt ccggaaggtg ttgtaaaggc tcgacgccca
     9241 aggtgggagt ctaggagaag aatttgcatc gggagtgggg cgggttaccc ctccatatcc
     9301 aatgacagat atctaccagc caagggtttg agcccgcccg cttagtcgtc gtcctcgctt
     9361 gcccctccat aaaaggattt cccctccccc tcccacaaaa ttttctttcc cttcctctcc
     9421 ttgtccgctt cagtacgtat atcttccctt ccctcgcttc tctcctccat ccttctttca
     9481 tccatctcct gctaacttct ctgctcagca cctctacgca ttactagccg tagtatctga
     9541 gcacttctcc cttttatatt ccacaaaaca taacacaacc ttcaccatat gaacaacggc
     9601 accaacaact tccagaactt catcggcatc tccagcctcc aaaagaccct ccgcaacgct
     9661 ctcatcccca ccgagactac ccagcagttc atcgtcaaga acggcatcat caaagaggat
     9721 gagctgcgcg gcgagaaccg ccagatcctc aaggatatca tggatgatta ctaccgcggc
     9781 tttatctccg agactctctc ctccatcgat gatatcgatt ggaccagcct cttcgagaag
     9841 atggaaatcc agctgaagaa cggcgataac aaggataccc tgatcaaaga gcagaccgag
     9901 taccgcaagg ccatccacaa gaagttcgcc aacgacgatc gcttcaagaa tatgttctcc
     9961 gccaagctca tctccgatat cctgcctgag ttcgtcatcc acaacaacaa ctactccgcc
    10021 tccgagaaag aggaaaagac ccaggtcatc aagctgttct cccgcttcgc cacctccttc
    10081 aaggattact tcaagaaccg cgccaactgc ttctccgccg acgatatctc ctccagctcc
    10141 tgccaccgca tcgtcaacga taacgccgag atcttcttct ccaacgctct ggtctaccgc
    10201 cgcatcgtga agtccctctc caacgatgac atcaacaaga tctccggcga tatgaaggat
    10261 tccctcaaag agatgtccct cgaggaaatc tactcctacg agaagtacgg cgagttcatt
    10321 acccaagagg gcatctcctt ctacaacgat atctgcggca aggtcaactc cttcatgaac
    10381 ctctactgcc agaagaacaa agagaacaag aacctgtaca agctccagaa gctccacaag
    10441 cagatcctct gtatcgccga taccagctac gaggtcccct acaagttcga gtccgatgag
    10501 gaagtctacc agtccgtcaa cggcttcctc gataacatca gctccaagca catcgtcgag
    10561 cgcctccgca agatcggcga caactacaac ggctacaacc tcgataagat ctacatcgtg
    10621 tccaagttct acgagtctgt ctcgcaaaag acctaccgcg attgggaaac catcaacacc
    10681 gctctcgaga tccattacaa caacatcttg cccggcaacg gcaagtccaa ggccgataag
    10741 gtcaagaagg ccgttaagaa cgatctgcag aagtccatca ccgagatcaa cgagctggtg
    10801 tccaactaca agctctgctc cgatgacaac atcaaggccg aaacctacat ccacgagatc
    10861 tcccacatcc tgaacaactt cgaggcccaa gagctgaagt acaaccccga gatccacctc
    10921 gtcgagagcg agctgaaggc ctccgagttg aagaacgtcc tcgatgtcat catgaacgcc
    10981 ttccactggt gctccgtgtt catgaccgag gaactcgtcg ataaggataa caacttctac
    11041 gccgagctgg aagaaatcta cgatgagatc taccccgtca tctccctgta caacctggtc
    11101 cgcaactacg tcacccagaa gccttactcc accaagaaga tcaagctcaa cttcggcatc
    11161 cccactctcg ccgatggttg gtccaagtcc aaagagtact ccaacaacgc catcatcctc
    11221 atgcgcgata acctgtacta cctcggcatc ttcaacgcca agaacaagcc cgataagaag
    11281 attatcgagg gcaacaccag cgagaacaag ggcgattaca agaagatgat ctacaacctg
    11341 ctgcctggtc ctaacaagat gatccccaag gtgttcctgt cctccaagac cggcgtcgag
    11401 acttacaagc cctccgctta catcctcgaa ggctacaagc agaacaagca cattaagtcc
    11461 agcaaggatt tcgatatcac cttctgccac gatctgatcg actatttcaa gaactgcatt
    11521 gccatccatc ctgagtggaa gaacttcggc ttcgatttct ccgatacctc cacctacgag
    11581 gatatctccg gcttctaccg cgaggtcgag ctgcagggct acaagatcga ctggacctac
    11641 atcagcgaga aggatatcga tctcctgcaa gagaagggcc agctctacct cttccagatc
    11701 tataacaaag atttctccaa gaagtccacc ggcaacgaca acctccacac catgtacctc
    11761 aagaacctct tcagcgagga aaacctcaag gacatcgtcc tcaagctgaa cggcgaggct
    11821 gaaatcttct tcagaaagtc ctccatcaag aaccccatca tccataagaa gggctccatc
    11881 ctcgtcaacc gcacctacga agccgaggaa aaggatcagt tcggcaacat ccagatcgtc
    11941 cgcaagaaca tccccgagaa catctaccaa gagttgtaca agtacttcaa cgacaagtcc
    12001 gacaaagagc tgtccgacga ggccgcaaag ctcaagaacg tcgtcggcca ccatgaggcc
    12061 gccaccaaca tcgttaagga ttaccgctat acctacgaca agtatttcct gcacatgccc
    12121 atcaccatca acttcaaggc caacaagacc ggtttcatca acgatcgcat cctccagtat
    12181 atcgccaaag agaaggatct ccacgtcatc ggtatcgatc gcggcgagcg caacctcatc
    12241 tacgtgtccg tcatcgatac ctgcggtaac atcgtggaac agaagtcctt caacatcgtc
    12301 aatggctacg attaccagat caagttgaag cagcaagagg gcgctcgcca gatcgctcgc
    12361 aaagagtgga aagagatcgg caagatcaaa gaaatcaaag agggctacct gagcctggtg
    12421 atccatgaga tttccaagat ggtgatcaag tacaacgcta tcattgccat ggaagatctg
    12481 tcctacggtt tcaagaaggg ccgcttcaag gtcgaacgcc aggtctacca aaagttcgaa
    12541 accatgctga tcaacaagtt gaactacctc gtctttaagg acatctccat taccgagaac
    12601 ggcggcctcc tcaagggcta ccagctcacc tacattcccg ataagctgaa aaacgtcggc
    12661 catcagtgcg gctgcatctt ctacgtccct gccgcctaca cctccaagat cgatcccacc
    12721 accggcttcg tcaacatctt caagttcaag gatctcaccg tcgatgccaa gcgcgaattc
    12781 atcaagaagt ttgattccat ccgctacgat tccgagaaga atctcttctg tttcaccttc
    12841 gactacaaca atttcatcac tcagaacacc gtcatgtcca agtcgtcctg gtccgtctac
    12901 acctacggcg tccgcatcaa gcgccgcttc gttaacggcc gcttctcgaa cgagtccgac
    12961 accatcgaca tcaccaagga tatggaaaag acactcgaga tgaccgatat caactggcgc
    13021 gacggccacg acctccgcca ggacatcatc gactacgaga tcgtccagca tatcttcgaa
    13081 atcttccgcc tcaccgtcca gatgcgcaac tccctgtccg agttggagga tcgcgactac
    13141 gatcgcctca tctcccctgt cctcaacgag aacaacattt tctacgatag cgccaaggcc
    13201 ggcgacgccc tgcctaagga tgccgacgcc aacggcgcct actgtatcgc tctcaagggc
    13261 ctgtacgaga tcaagcaaat caccgaaaac tggaaagagg acggaaagtt cagccgcgac
    13321 aagctgaaga tctccaacaa ggattggttc gatttcatcc aaaacaagcg ctacctgcct
    13381 cccaagaaga agcgcaaggt ctgaagcgga cattcgattt atgccgttat gacttcctta
    13441 aaaaagcctt tacgaatgaa agaaatggaa ttagacttgt tatgtagttg attctacaat
    13501 ggattatgat tcctgaactt caaatccgct gttcattatt aatctcagct cttcccgtaa
    13561 agccaatgtt gaaactattc gtaaatgtac ctcgttttgc gtgtaccttg cttatcacgt
    13621 gatattacat gacctggaca gagttctgcg cgaaagtcat aacgtaaatc ccgggcggta
    13681 ggtgcgtccc gggcggaagg tagttttctc gtccacccca acgcgtttat caacctcaac
    13741 tttcaacaac catcatgcca ccaaaagcgc gtaaaacaaa gcgagatttg attgagcaag
    13801 agggcaggat ccaatgcgcg attcaagaca ttaaaaatgg aaaatttcaa aaaattgcgc
    13861 ccgcagcgcg tgcatacaaa attcatccca atacattaag acctcagcgc tagtggaggt
    13921 caacacatca atgcctattt tggtttagtc gtccaggcgg tgagcacaaa atttgtgtcg
    13981 tttgacaaga tggttcattt aggcaactgg tcagatcagc cccacttgta gcagtagcgg
    14041 cggcgctcga agtgtgactc ttattagcag acaggaacga ggacattatt atcatctgct
    14101 gcttggtgca cgataacttg gtgcgtttgt caagcaaggt aagtggacga cccggtcata
    14161 ccttcttaag ttcgcccttc ctccctttat ttcagattca atctgactta cctattctac
    14221 ccaagcatcc aaatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
    14281 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
    14341 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
    14401 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    14461 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
    14521 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
    14581 cacttcgtgg ccgaggagca ggactgaccg acgccgacca acaccgccgg tccgacgcgg
    14641 cccgacgggt ccgaggagct tgagatccac ttaacgttac tgaaatcatc aaacagcttg
    14701 acgaatctgg atataagatc gttggtgtcg atgtcagctc cggagttgag acaaatggtg
    14761 ttcaggatct cgataagata cgttcatttg tccaagcagc aaagagtgcc ttctagtgat
    14821 ttaatagctc catgtcaaca agaataaaac gcgtttcggg tttacctctt ccagatacag
    14881 ctcatctgca atgcattaat gcattggacc tcgcaaccct agtacgccct tcaggctccg
    14941 gcgaagcaga agaatagctt agcagagtct attttcattt tcgggagacg agatcaagca
    15001 gatcaacggt cgtcaagaga cctacgagac tgaggaatcc gctcttggct ccacgcgact
    15061 atatatttgt ctctaattgt actttgacat gctcctcttc tttactctga tagcttgact
    15121 atgaaaattc cgtcaccagc ccctgggttc gcaaagataa ttgcactgtt tcttccttga
    15181 actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaaa tcattcctac
    15241 taagatgggt atacaatagt aaccatgcat ggttgcctag tgaatgctcc gtaacaccca
    15301 atacgccggc cgaaactttt ttacaactct cctatgagtc gtttacccag aatgcacagg
    15361 tacacttgtt tag
//
