LOCUS       sequence-454126-       14494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Vanegas KG, Rendsvig JKH, Jarczynska ZD, Cortes MVCB, van Esch AP, 
            Morera-Gomez M, Contesini FJ, Mortensen UH
  TITLE     A Mad7 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   J Fungi (Basel). 2022 Dec 22;9(1):16. doi: 10.3390/jof9010016.
  PUBMED    36675838
REFERENCE   2  (bases 1 to 14494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2022-12-22"; volume: "9(1):16. doi"; pages: " 
            10.3390/jof9010016"
COMMENT     Sequence Label: pDIV302
FEATURES             Location/Qualifiers
     source          1..14494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6840
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             11829..11849
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     rep_origin      complement(13783..14371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13863..13882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14493..14494,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt tggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atatgggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aaaacaagac agccacatat tagggagact agttagaagc tagttccagg actaggaaaa
     1981 taaaagacaa tgataccaca gtctagttga caactagata gattctagat tgaggccaaa
     2041 gtcyctgaga tccaggttag ttgcaactaa tactagttag tatctagtct cctataactc
     2101 tgaagctaga ataacttact actattatcc tcaccactgt tcagctgcgc aaacggagtg
     2161 attgcaaggt gttcagagac tagttattga ctagtcagtg actagcaata actaacaagg
     2221 tattaaccta ccatgtctgc catcaccctg cacttcctcg ggctcagcag ccttttcctc
     2281 ctcattttca tgctcatttt ccttgtttaa gactgtgact agtcaaagac tagtccagaa
     2341 ccacaaagga gaaatgtctt accactttct tcattgcttg tctcttttgc attatccatg
     2401 tctgcaacta gttagagtct agttagtgac tagtccgacg aggacttgct tgtctccgga
     2461 ttgttggagg aactctccag ggcctcaaga tccacaacag agccttctag aagactggtc
     2521 aataactagt tggtctttgt ctgagtctga cttacgaggt tgcatactcg ctccctttgc
     2581 ctcgtcaatc gatgagaaaa agcgccaaaa ctcgcaatat ggctttgaac cacacggtgc
     2641 tgagactagt tagaatctag tcccaaacta gcttkgatag cttacctttg ccctttgcgt
     2701 tgcgacaggt cttgcagggt atggttcctt tctcaccagc tgatttagct gccttgctac
     2761 cctcacggcg gatctgcata aagagtggct agaggttata aattagcact gatcctaggt
     2821 acggggctga atgtaacttg ccyttccttt ctcatcgcgc ggcaagacag gcttgctcaa
     2881 attcctacca gtcacagggg tatgcacggc gtacggacca cttgaactag tcacagatta
     2941 gttagcaact agtctgcatt gaatggctgt acttacgggc cctcgccatt gtcctgatca
     3001 tttccagctt caccctcgtt gctgcaaagt agttagtgac tagtcaagga ctagttgaaa
     3061 tgggagaaga aactcacgaa ttctcgacac ccttagtatt gtggtccttg gacttggtgc
     3121 tgctatatat tagctaatac actagttaga ctcacagaaa cttacgcagc tcgcttgcgc
     3181 ttcttggtag gagtcggggt tgggagaaca gtgccttcaa acaagccttc ataccatgct
     3241 acttgactag tcagggacta gtcaccaagt aatctagata ggacttgcct ttggcctcca
     3301 tcagttcctt catagtggga ggtccattgt gcaatgtaaa ctccatgccg tgggagttct
     3361 tgtccttcaa gtgcttgacc aatatgtttc tgttggcaga gggaacctgt caactagtta
     3421 ataactagtc agaaactagt atagcagtag actcactgta cgcttgaggc atcccttcac
     3481 tcggcagtag acttcatatg gatggatatc aggcacgcca ttgtcgtcct gtggactagt
     3541 cagtaactag gcttaaagct agtcgggtcg gcttactatc ttgaaatccg gcagcgtaag
     3601 ctccccgtcc ttaactgcct cgagatagtg acagtactct ggggactttc ggagatcgtt
     3661 atcgcgaatg ctcggcatac taatcgttga ctagtcttgg actagtcccg agcaaaaagg
     3721 attggaggag gaggaggaag gtgagagtga gacaaagagc gaaataagag cttcaaaggc
     3781 tatctctaag cagtatgaag gttaagtatc tagttcttga ctagatttaa aagagatttc
     3841 aactagttat gtacctggag tttggatata ggaatgtgtt gtggtaacga aatgtaaggg
     3901 ggaggaaaga aaaagtcggt caagaggtaa ctctaagtcg gccattcctt tttgggaggc
     3961 gctaaccata aacggcatgg tcgacttaga gttagctcag ggaatttagg gagttatctg
     4021 cgaccaccga ggaacggcgg aatgccaaag aatcccgatg gagctctagc tggcggttga
     4081 caaccccacc ttttggcgtt tctgcggcgt tgcaggcggg actggatact tcgtagaacc
     4141 agaaaggcaa ggcagaacgc gctcagcaag agtgttggaa gtgatagcat gatgtgcctt
     4201 gttaactagg tcaaaatctg cagtatgctt gatgttatcc aaagtgtgag agaggaaggt
     4261 ccaaacatac acgattggga gagggcctag gtataagagt ttttgagtag aacgcatgtg
     4321 agcccagcca tctcgaggag attaaacacg ggccggcatt tgatggctat gttagtaccc
     4381 caatggaaac ggtgagagtc cagtggtcgc agataactcc ctaaattccc tgagctaact
     4441 ctaagtcgac catgccgttt atggttagcg cctcccaaaa aggaatggcc gacttagagt
     4501 tacctcttga ccgacttttt ctttcctccc ccttacattt cgttaccaca acacattcct
     4561 atatccaaac tccaggtaca taactagtcg aaatctcttt taaatctagt caagaactag
     4621 atacttaacc ttcatactgc ttagagatag cctttgaagc tcttatttcg ctctttgtct
     4681 cactctcacc ttcctcctcc tcctccaatc ctttttgctc gggactagtc caagactagt
     4741 caacgattag tatgccgagc attcgcgata acgatctccg aaagtcccca gagtactgtc
     4801 actatctcga ggcagttaag gacggggagc ttacgctgcc ggatttcaag atagtaagcc
     4861 gacccgacta gctttaagcc tagttactga ctagtccaca ggacgacaat ggcgtgcctg
     4921 atatccatcc atatgaagtc tactgccgag tgaagggatg cctcaagcgt acagtgagtc
     4981 tactgctata ctagtttctg actagttatt aactagttga caggttccct ctgccaacag
     5041 aaacatattg gtcaagcact tgaaggacaa gaactcccac ggcatggagt ttacattgca
     5101 caatggacct cccactatga aggaactgat ggaggccaaa ggcaagtcct atctagatta
     5161 cttggtgact agtccctgac tagtcaagta gcatggtatg aaggcttgtt tgaaggcact
     5221 gttctcccaa ccccgactcc taccaagaag cgcaagcgag ctgcgtaagt ttctgtgagt
     5281 ctaactagtg tattagctaa tatatagcag caccaagtcc aaggaccaca atactaaggg
     5341 tgtcgagaat tcgtgagttt cttctcccat ttcaactagt ccttgactag tcactaacta
     5401 ctttgcagca acgagggtga agctggaaat gatcaggaca atggcgaggg cccgtaagta
     5461 cagccattca atgcagacta gttgctaact aatctgtgac tagttcaagt ggtccgtacg
     5521 ccgtgcatac ccctgtgact ggtaggaatt tgagcaagcc tgtcttgccg cgcgatgaga
     5581 aaggaarggc aagttacatt cagccccgta cctaggatca gtgctaattt ataacctcta
     5641 gccactcttt atgcagatcc gccgtgaggg tagcaaggca gctaaatcag ctggtgagaa
     5701 aggaaccata ccctgcaaga cctgtcgcaa cgcaaagggc aaaggtaagc tatcmaagct
     5761 agtttgggac tagattctaa ctagtctcag caccgtgtgg ttcaaagcca tattgcgagt
     5821 tttggcgctt tttctcatcg attgacgagg caaagggagc gagtatgcaa cctcgtaagt
     5881 cagactcaga caaagaccaa ctagttattg accagtcttc tagaaggctc tgttgtggat
     5941 cttgaggccc tggagagttc ctccaacaat ccggagacaa gcaagtcctc gtcggactag
     6001 tcactaacta gactctaact agttgcagac atggataatg caaaagagac aagcaatgaa
     6061 gaaagtggta agacatttct cctttgtggt tctggactag tctttgacta gtcacagtct
     6121 taaacaagga aaatgagcat gaaaatgagg aggaaaaggc tgctgagccc gaggaagtgc
     6181 agggtgatgg cagacatggt aggttaatac cttgttagtt attgctagtc actgactagt
     6241 caataactag tctctgaaca ccttgcaatc actccgtttg cgcagctgaa cagtggtgag
     6301 gataatagta gtaagttatt ctagcttcag agttatagga gactagatac taactagtat
     6361 tagttgcaac taacctggat ctcagrgact ttggcctcaa tctagaatct atctagttgt
     6421 caactagact gtggtatcat tgtcttttat tttcctagtc ctggaactag cttctaacta
     6481 gtctccctaa tatgtggctg tcttgttttt ttttttttgt ttccctaccc ggatatctag
     6541 tccccttcta ggttctgtta acctctcggg ctctgattta gtttaacgca aacctgagat
     6601 tagtttctaa ctagtctcta ggttttctat ccacctttaa ttgtaataat aaatacaagc
     6661 aacgtttata cgtcaaaagc atttataaac ttttacccta aagtagcttg cttgtgtgtt
     6721 tagtttataa ttagtctctt attaatttga tgtaggtaag cccgccacaa atatatattt
     6781 ttaacaagat accgtggaaa aacttcgtgc tatcacaaaa cagtatacaa aaaataagct
     6841 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
     6901 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
     6961 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
     7021 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
     7081 catggtcata gctggctgag ggtttaatgc tgagggttta atgctgaggg tttaattaag
     7141 acctcagccg agacagcaga atcaccgccc aagttaagcc tttgtgctga tcatgctctc
     7201 gaacgggcca agttcgggaa aagcaaagga gcgtttagtg aggggcaatt tgactcacct
     7261 cccaggcaac agatgagggg ggcaaaaaga aagaaatttt cgtgagtcaa tatggattcc
     7321 gagcatcatt ttcttgcggt ctatcttgct acgtatgttg atcttgacgc tgtggatcaa
     7381 gcaacgccac tcgctcgctc catcgcaggc tggtcgcaga caaattaaaa ggcggcaaac
     7441 tcgtacagcc gcggggttgt ccgctgcaaa gtacagagtg ataaaagccg ccatgcgacc
     7501 atcaacgcgt tgatgcccag ctttttcgat ccgagaatcc accgtagagg cgatagcaag
     7561 taaagaaaag ctaaacaaaa aaaaatttct gcccctaagc catgaaaacg agatggggtg
     7621 gagcagaacc aaggaaagag tcgcgctggg ctgccgttcc ggaaggtgtt gtaaaggctc
     7681 gacgcccaag gtgggagtct aggagaagaa tttgcatcgg gagtggggcg ggttacccct
     7741 ccatatccaa tgacagatat ctaccagcca agggtttgag cccgcccgct tagtcgtcgt
     7801 cctcgcttgc ccctccataa aaggatttcc cctccccctc ccacaaaatt ttctttccct
     7861 tcctctcctt gtccgcttca gtacgtatat cttcccttcc ctcgcttctc tcctccatcc
     7921 ttctttcatc catctcctgc taacttctct gctcagcacc tctacgcatt actagccgta
     7981 gtatctgagc acttctccct tttatattcc acaaaacata acacaacctt caccatatga
     8041 acaacggcac caacaacttc cagaacttca tcggcatctc cagcctccaa aagaccctcc
     8101 gcaacgctct catccccacc gagactaccc agcagttcat cgtcaagaac ggcatcatca
     8161 aagaggatga gctgcgcggc gagaaccgcc agatcctcaa ggatatcatg gatgattact
     8221 accgcggctt tatctccgag actctctcct ccatcgatga tatcgattgg accagcctct
     8281 tcgagaagat ggaaatccag ctgaagaacg gcgataacaa ggataccctg atcaaagagc
     8341 agaccgagta ccgcaaggcc atccacaaga agttcgccaa cgacgatcgc ttcaagaata
     8401 tgttctccgc caagctcatc tccgatatcc tgcctgagtt cgtcatccac aacaacaact
     8461 actccgcctc cgagaaagag gaaaagaccc aggtcatcaa gctgttctcc cgcttcgcca
     8521 cctccttcaa ggattacttc aagaaccgcg ccaactgctt ctccgccgac gatatctcct
     8581 ccagctcctg ccaccgcatc gtcaacgata acgccgagat cttcttctcc aacgctctgg
     8641 tctaccgccg catcgtgaag tccctctcca acgatgacat caacaagatc tccggcgata
     8701 tgaaggattc cctcaaagag atgtccctcg aggaaatcta ctcctacgag aagtacggcg
     8761 agttcattac ccaagagggc atctccttct acaacgatat ctgcggcaag gtcaactcct
     8821 tcatgaacct ctactgccag aagaacaaag agaacaagaa cctgtacaag ctccagaagc
     8881 tccacaagca gatcctctgt atcgccgata ccagctacga ggtcccctac aagttcgagt
     8941 ccgatgagga agtctaccag tccgtcaacg gcttcctcga taacatcagc tccaagcaca
     9001 tcgtcgagcg cctccgcaag atcggcgaca actacaacgg ctacaacctc gataagatct
     9061 acatcgtgtc caagttctac gagtctgtct cgcaaaagac ctaccgcgat tgggaaacca
     9121 tcaacaccgc tctcgagatc cattacaaca acatcttgcc cggcaacggc aagtccaagg
     9181 ccgataaggt caagaaggcc gttaagaacg atctgcagaa gtccatcacc gagatcaacg
     9241 agctggtgtc caactacaag ctctgctccg atgacaacat caaggccgaa acctacatcc
     9301 acgagatctc ccacatcctg aacaacttcg aggcccaaga gctgaagtac aaccccgaga
     9361 tccacctcgt cgagagcgag ctgaaggcct ccgagttgaa gaacgtcctc gatgtcatca
     9421 tgaacgcctt ccactggtgc tccgtgttca tgaccgagga actcgtcgat aaggataaca
     9481 acttctacgc cgagctggaa gaaatctacg atgagatcta ccccgtcatc tccctgtaca
     9541 acctggtccg caactacgtc acccagaagc cttactccac caagaagatc aagctcaact
     9601 tcggcatccc cactctcgcc gatggttggt ccaagtccaa agagtactcc aacaacgcca
     9661 tcatcctcat gcgcgataac ctgtactacc tcggcatctt caacgccaag aacaagcccg
     9721 ataagaagat tatcgagggc aacaccagcg agaacaaggg cgattacaag aagatgatct
     9781 acaacctgct gcctggtcct aacaagatga tccccaaggt gttcctgtcc tccaagaccg
     9841 gcgtcgagac ttacaagccc tccgcttaca tcctcgaagg ctacaagcag aacaagcaca
     9901 ttaagtccag caaggatttc gatatcacct tctgccacga tctgatcgac tatttcaaga
     9961 actgcattgc catccatcct gagtggaaga acttcggctt cgatttctcc gatacctcca
    10021 cctacgagga tatctccggc ttctaccgcg aggtcgagct gcagggctac aagatcgact
    10081 ggacctacat cagcgagaag gatatcgatc tcctgcaaga gaagggccag ctctacctct
    10141 tccagatcta taacaaagat ttctccaaga agtccaccgg caacgacaac ctccacacca
    10201 tgtacctcaa gaacctcttc agcgaggaaa acctcaagga catcgtcctc aagctgaacg
    10261 gcgaggctga aatcttcttc agaaagtcct ccatcaagaa ccccatcatc cataagaagg
    10321 gctccatcct cgtcaaccgc acctacgaag ccgaggaaaa ggatcagttc ggcaacatcc
    10381 agatcgtccg caagaacatc cccgagaaca tctaccaaga gttgtacaag tacttcaacg
    10441 acaagtccga caaagagctg tccgacgagg ccgcaaagct caagaacgtc gtcggccacc
    10501 atgaggccgc caccaacatc gttaaggatt accgctatac ctacgacaag tatttcctgc
    10561 acatgcccat caccatcaac ttcaaggcca acaagaccgg tttcatcaac gatcgcatcc
    10621 tccagtatat cgccaaagag aaggatctcc acgtcatcgg tatcgatcgc ggcgagcgca
    10681 acctcatcta cgtgtccgtc atcgatacct gcggtaacat cgtggaacag aagtccttca
    10741 acatcgtcaa tggctacgat taccagatca agttgaagca gcaagagggc gctcgccaga
    10801 tcgctcgcaa agagtggaaa gagatcggca agatcaaaga aatcaaagag ggctacctga
    10861 gcctggtgat ccatgagatt tccaagatgg tgatcaagta caacgctatc attgccatgg
    10921 aagatctgtc ctacggtttc aagaagggcc gcttcaaggt cgaacgccag gtctaccaaa
    10981 agttcgaaac catgctgatc aacaagttga actacctcgt ctttaaggac atctccatta
    11041 ccgagaacgg cggcctcctc aagggctacc agctcaccta cattcccgat aagctgaaaa
    11101 acgtcggcca tcagtgcggc tgcatcttct acgtccctgc cgcctacacc tccaagatcg
    11161 atcccaccac cggcttcgtc aacatcttca agttcaagga tctcaccgtc gatgccaagc
    11221 gcgaattcat caagaagttt gattccatcc gctacgattc cgagaagaat ctcttctgtt
    11281 tcaccttcga ctacaacaat ttcatcactc agaacaccgt catgtccaag tcgtcctggt
    11341 ccgtctacac ctacggcgtc cgcatcaagc gccgcttcgt taacggccgc ttctcgaacg
    11401 agtccgacac catcgacatc accaaggata tggaaaagac actcgagatg accgatatca
    11461 actggcgcga cggccacgac ctccgccagg acatcatcga ctacgagatc gtccagcata
    11521 tcttcgaaat cttccgcctc accgtccaga tgcgcaactc cctgtccgag ttggaggatc
    11581 gcgactacga tcgcctcatc tcccctgtcc tcaacgagaa caacattttc tacgatagcg
    11641 ccaaggccgg cgacgccctg cctaaggatg ccgacgccaa cggcgcctac tgtatcgctc
    11701 tcaagggcct gtacgagatc aagcaaatca ccgaaaactg gaaagaggac ggaaagttca
    11761 gccgcgacaa gctgaagatc tccaacaagg attggttcga tttcatccaa aacaagcgct
    11821 acctgcctcc caagaagaag cgcaaggtct gaagcggaca ttcgatttat gccgttatga
    11881 cttccttaaa aaagccttta cgaatgaaag aaatggaatt agacttgtta tgtagttgat
    11941 tctacaatgg attatgattc ctgaacttca aatccgctgt tcattattaa tctcagctct
    12001 tcccgtaaag ccaatgttga aactattcgt aaatgtacct cgttttgcgt gtaccttgct
    12061 tatcacgtga tattacatga cctggacaga gttctgcgcg aaagtcataa cgtaaatccc
    12121 gggcggtagg tgcgtcccgg gcggaaggta gttttctcgt ccaccccaac gcgtttatca
    12181 acctcaactt tcaacaacca tcatgccacc aaaagcgcgt aaaacaaagc gagatttgat
    12241 tgagcaagag ggcaggatcc aatgcgcgat tcaagacatt aaaaatggaa aatttcaaaa
    12301 aattgcgccc gcagcgcgtg catacaaaat tcatcccaat acattaagac ctcagcgcta
    12361 gtggaggtca acacatcaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat
    12421 ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc
    12481 agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat
    12541 catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtggacgacc
    12601 cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc
    12661 tattctaccc aagcatccaa aaacaaccat gggaaccacc ttggatgaca ctgcttatcg
    12721 ataccgaaca tctgtacctg gtgatgctga agctatcgaa gcccttgacg ggtcattcac
    12781 aactgacacc gtctttagag ttacagctac tggggatggg tttacattgc gagaagtacc
    12841 agtagatcct cctttgacca aagtattccc agatgacgaa agtgacgatg aatccgatga
    12901 cggtgaggac ggtgacccag actcaagaac atttgtcgct tatggagacg acggtgattt
    12961 ggccggtttc gtagttgtat cctattctgg atggaaccga agacttactg tcgaggatat
    13021 agaggttgcc ccagaacatc gtgggcacgg tgttgggcga gcccttatgg gattagctac
    13081 agaatttgcc cgtgaacgtg gggctggaca tttatggtta gaagtcacca atgttaatgc
    13141 accagctata catgcatatc gaagaatggg gttcacactt tgtggattgg atacagcttt
    13201 atacgatggt acagcctcag acggagaaca agccttatac atgtctatgc cttgtccata
    13261 atcagtactc cgacgccgac caacaccgcc ggtccgacgc ggcccgacgg gtccgaggag
    13321 cttgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga
    13381 tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga
    13441 tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa
    13501 caagaataaa acgcgtttcg ggtttacctc ttccagatac agctcatctg caatgcatta
    13561 atgcattgga cctcgcaacc ctagtacgcc cttcaggctc cggcgaagca gaagaatagc
    13621 ttagcagagt ctattttcat tttcgggaga cgagatcaag cagatcaacg gtcgtcaaga
    13681 gacctacgag actgaggaat ccgctcttgg ctcattaaga cctcagcgag caaaaggcca
    13741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    13801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    13861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    13921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    13981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    14041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    14101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    14161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag
    14221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    14281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    14341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    14401 tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg
    14461 cgccgtcccg tcaagtcagc gtaatgctct gctt
//
