LOCUS       sequence-454161-       17127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for 
            monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was 
            driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 17127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT17
FEATURES             Location/Qualifiers
     source          1..17127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3273..3338
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3345..3365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3390..3413,3414..3416,3417..7490)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7403..7411
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7491..7538
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7560..7808
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7821..7841
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7863..8115
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8160..8180
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        8217..8594
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             complement(8565..8897)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLCHG
                     SSAQLF"
     primer_bind     8762..8778
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8788..8807
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8788..8806
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8854..8874)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8854..8872)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8893..8909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8917..8933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8941..8947,8948..8965,8966..8971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8986..9007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        9049..9124
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9213..9237)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9305..9321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9329..9345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9353..9359,9360..9377,9378..9383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9398..9419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9610..10286
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10223..10243
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10331..10882
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    10889..11063
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11141..11165
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11590..12384
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12471..13059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12960..12979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13202..13219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13234..13374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13300..13319)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13460..13482
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13718..13912
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13978..15051)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15480..16109)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     1381 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 acccgtcggc acctccgctt caaggtacgc cgctcgtccy cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag caggctccga attcgccctt catggaggcc tctccggctt
     2641 caggtcctcg ccatctcatg gacccccata tctttacctc caatttcaat aacggcattg
     2701 gcaggcacaa gacatacctc tgttatgagg tcgagcggct cgataacgga acctcagtga
     2761 agatggacca acacaggggt ttccttcata accaagcgaa aaatctcctt tgcggtttct
     2821 atgggcggca cgcggagctg agattcttgg atttggtccc gagcctccag ctggatcccg
     2881 cgcaaatata ccgcgtcact tggttcatat catggagccc gtgctttagc tgggggtgtg
     2941 ccggtgaggt tcgcgcgttc ctccaggaaa acacccatgt tcgcctccgg attttcgcag
     3001 cgaggatttt cgactatgat ccactttaca aagaggccct ccaaatgctt agagatgctg
     3061 gggctcaagt ttcaattatg acatacgacg agtttaagca ttgttgggac acttttgtcg
     3121 atcatcaggg ttgtcccttc cagccgtggg atggtcttga tgagcattcg caagctttga
     3181 gtggcaggct tcgcgcgata cttcagaacc agggtaactc aggttctgag acgcctggca
     3241 caagtgagtc agcaacaccc gagtccacca tggattacaa ggaccacgac ggggattaca
     3301 aggaccacga cattgattac aaggatgatg atgacaagat ggctccgaag aagaagagga
     3361 aggttggcat ccacggggtg ccagctgctg acaagaagta ctcgatcggc ctcgctattg
     3421 ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt
     3481 tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc
     3541 tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt
     3601 acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga
     3661 aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga
     3721 agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt
     3781 accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc
     3841 gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg
     3901 gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt
     3961 acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc
     4021 tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg
     4081 agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact
     4141 tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg
     4201 acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg
     4261 ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga
     4321 ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc
     4381 tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct
     4441 tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt
     4501 tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga
     4561 agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac
     4621 accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt
     4681 tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg
     4741 tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga
     4801 cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca
     4861 tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact
     4921 cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg
     4981 agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc
     5041 tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga
     5101 ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg
     5161 ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga
     5221 atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga
     5281 tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca
     5341 agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg
     5401 acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca
     5461 atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc
     5521 aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag
     5581 ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg
     5641 gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga
     5701 agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg
     5761 ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct
     5821 acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca
     5881 ggctcagcga ttacgacgtc gatcatatcg ttccacagtc attcctgaag gatgactcca
     5941 ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt
     6001 cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga
     6061 tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg
     6121 acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg
     6181 cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg
     6241 aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt
     6301 tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg
     6361 tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg
     6421 actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg
     6481 ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc
     6541 tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga
     6601 tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag
     6661 tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattctgc
     6721 ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg
     6781 gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg
     6841 gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct
     6901 ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga
     6961 aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc
     7021 ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg ccgtccaagt
     7081 atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca
     7141 acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc
     7201 agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct
     7261 ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc
     7321 tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac acaactatcg
     7381 atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc caccagtcga
     7441 ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac aagcggccag
     7501 cggcgacgaa gaaggcgggg caggcgaaga agaagaaggg agacggatca ggcgggtcaa
     7561 caaacctcag tgacatcata gagaaggaaa ctggtaagca actggttatt caagagagca
     7621 ttctgatgct ccctgaggaa gtcgaggaag ttataggaaa caagcctgag agcgatatac
     7681 tcgtgcatac ggcgtatgac gagagcacgg atgaaaatgt gatgctcttg accagcgatg
     7741 cgccagaata taaaccctgg gcactggtta ttcaggactc taacggggaa aataaaatca
     7801 agatgctctc aggtggctcc ccaaagaaga aacgcaaggt ttagtggcgg ccgccgacgt
     7861 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     7921 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     7981 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     8041 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     8101 ctatgttact agatcgggaa ttgatccccc ctcgacagct tccggaaagg gcgaattcgc
     8161 aactttgtat acaaaagttg ccccatggcg ttccctctag ataacgcagg atcctaaagg
     8221 gatctttaaa catacgaaca gatcacttaa agttcttctg aagcaactta aagttatcag
     8281 gcatgcatgg atcttggagg aatcagatgt gcagtcaggg accatagcac aggacaggcg
     8341 tcttctactg gtgctaccag caaatgctgg aagccgggaa cactgggtac gttggaaacc
     8401 acgtgatgtg gagtaagata aactgtagga gaaaagcatt tcgtagtggg ccatgaagcc
     8461 tttcaggaca tgtattgcag tatgggccgg cccattacgc aattggacga caacaaagac
     8521 tagtattagt accacctcgg ctatccacat agatcaaagc tggtttaaaa gagttgtgca
     8581 gatgatccgt ggcagagacc ggcgccgcta cagggcgcgt cccattcgcc attcaggctg
     8641 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8701 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8761 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     8821 gggccccccc tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     8881 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8941 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9001 cacattaatt gcgttgcgct cactgcccgc tttccaccgg tggtctctgt tttagagcta
     9061 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     9121 gtgctttttt tgtcccttcg aagggcaatt ctgcagatat ccatcacact ggcggccgct
     9181 cgaggtcgag ggtatcgata agcttgaatt cgacccagct ttcttgtaca aagtggttcg
     9241 ataattctta attaactagt tctagagcgg ccgccaccgc ggtggagctc gaattcgtaa
     9301 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9361 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9421 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat
     9541 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     9601 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     9661 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     9721 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     9781 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     9841 aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata
     9901 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
     9961 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
    10021 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
    10081 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
    10141 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
    10201 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
    10261 catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct
    10321 cgagtctacc atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga
    10381 catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg
    10441 taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta
    10501 tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggcccctg
    10561 gaaggcacgc aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca
    10621 ccagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca
    10681 gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca
    10741 cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa
    10801 ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtaccgc cccgtccggt
    10861 cctgcccgtc accgagattt gactcgagtt tctccataat aatgtgtgag tagttcccag
    10921 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
    10981 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
    11041 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
    11101 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
    11161 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
    11221 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
    11281 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
    11341 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
    11401 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
    11461 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
    11521 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
    11581 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
    11641 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
    11701 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
    11761 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
    11821 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
    11881 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
    11941 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
    12001 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
    12061 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
    12121 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
    12181 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
    12241 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
    12301 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
    12361 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
    12421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    12481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    12541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12601 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
    12661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    13021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    13081 ttacggttcc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    13141 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    13201 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
    13261 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
    13321 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
    13381 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
    13441 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
    13501 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
    13561 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
    13621 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
    13681 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
    13741 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
    13801 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
    13861 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaacag
    13921 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
    13981 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
    14041 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
    14101 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
    14161 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
    14221 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
    14281 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
    14341 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
    14401 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
    14461 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
    14521 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
    14581 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
    14641 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
    14701 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
    14761 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
    14821 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
    14881 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
    14941 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
    15001 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
    15061 tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct
    15121 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
    15181 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
    15241 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
    15301 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
    15361 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
    15421 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
    15481 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
    15541 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
    15601 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    15661 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    15721 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    15781 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    15841 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    15901 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    15961 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    16021 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    16081 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    16141 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    16201 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    16261 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    16321 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    16381 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    16441 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    16501 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    16561 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    16621 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    16681 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    16741 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    16801 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    16861 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    16921 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    16981 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    17041 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    17101 atgtctccgg ttctagtcgc aagtatt
//
