LOCUS       sequence-454162-        6356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses norovirus GI.1 VP1 protein in Sf9 insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Verardi R, Lindesmith LC, Tsybovsky Y, Gorman J, Chuang GY, Edwards 
            CE, Brewer-Jensen PD, Mallory ML, Ou L, Schon A, Shi W, Tully ES, 
            Georgiou G, Baric RS, Kwong PD
  TITLE     Disulfide stabilization of human norovirus GI.1 virus-like particles
            focuses immune response toward blockade epitopes.
  JOURNAL   NPJ Vaccines. 2020 Dec 14;5(1):110. doi: 10.1038/s41541-020-00260-w.
  PUBMED    33318483
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ 
            Vaccines."; date: "2020-12-14"; volume: "5(1):110. doi"; pages: " 
            10.1038/s41541-020-00260-w"
COMMENT     Sequence Label: pFastBac1_GI.1_WT
FEATURES             Location/Qualifiers
     source          1..6356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      66..654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     555..574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     808..825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  959..1183
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1250..1783)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1972..1977,1978..1994,1995..2000))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2352..2443
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2368..2385
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4294..4428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4331..4350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4385..4404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4457..4622
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4806..5260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4893..4912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5102..5123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5286..5390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5391..5459,5460..6251)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5609..5628)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
       61 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      181 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      721 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      781 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      841 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agaccagccg
      901 cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg taagcgggtg
      961 tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct
     1021 taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac
     1081 tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt
     1141 attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt tgtgacaatt
     1201 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     1261 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     1321 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     1381 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     1441 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     1501 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     1561 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     1621 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     1681 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     1741 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     1801 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     1861 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     1921 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     1981 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     2041 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     2101 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     2161 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     2221 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     2281 catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctacg tatactccgg
     2341 aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt
     2401 ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata
     2461 ccgtcccacc atcgggcgcg gatccatgat gatggcttca aaggacgcta cttcttctgt
     2521 ggacggtgct tctggagctg gtcagctggt cccagaggtg aacgcttccg acccactggc
     2581 catggaccct gtggctggct cttcaaccgc tgtggccact gctggacagg tcaaccccat
     2641 cgacccatgg atcatcaaca acttcgtgca ggcccctcag ggcgagttca ctatctcacc
     2701 caacaacacc ccaggtgacg tcctgttcga cctgtccctg ggccctcacc tgaacccctt
     2761 cctgctgcac ctgagccaga tgtacaacgg ctgggtcgga aacatgaggg tgagaatcat
     2821 gctggccggt aacgctttca ccgccggcaa gatcatcgtc tcatgcatcc ctcccggctt
     2881 cggttcccac aacctgacta tcgcccaggc taccctgttc ccacacgtca tcgctgacgt
     2941 gaggactctg gacccaatcg aggtgcctct ggaagacgtc agaaacgtgc tgttccacaa
     3001 caacgacagg aaccagcaga ccatgagact ggtctgcatg ctgtacaccc ctctgaggac
     3061 tggtggcgga actggagaca gcttcgtggt cgccggtcgt gtgatgactt gcccatctcc
     3121 tgacttcaac ttcctgttcc tggtcccacc taccgtggag cagaagacta ggccattcac
     3181 cctgcctaac ctgcccctgt ccagcctgtc caacagcaga gctcccctgc caatctcttc
     3241 aatgggcatc agccctgaca acgtccagtc tgtgcagttc cagaacggac gctgcactct
     3301 ggacggacgt ctggtcggta ccactcccgt gtctctgtca cacgtcgcca agatcagggg
     3361 cacttccaac ggaaccgtga tcaacctgac tgagctggac ggtaccccat tccacccttt
     3421 cgaaggccct gctcccatcg gattccctga cctgggtggc tgcgactggc acatcaacat
     3481 gactcagttc ggccactcca gccagaccca gtacgacgtg gacaccactc ccgacacctt
     3541 cgtcccacac ctgggtagca tccaggccaa cggtatcggc tctggaaact acgtgggcgt
     3601 cctgtcttgg atctcacccc catcccaccc atccggaagc caggtggacc tgtggaagat
     3661 ccctaactac ggttcttcaa tcaccgaggc tactcacctg gccccctctg tctaccctcc
     3721 cggtttcggc gaagtgctgg tcttcttcat gagcaagatg ccaggacctg gtgcttacaa
     3781 cctgccctgc ctgctgccac aggagtacat ctcacacctc gcttctgagc aggctccaac
     3841 tgtgggtgaa gctgccctgc tgcactacgt cgacccagac accggtcgca acctgggcga
     3901 gttcaaggcc taccctgacg gattcctgac ttgcgtgccc aacggagctt ccagcggtcc
     3961 tcagcagctg cccatcaacg gagtgttcgt cttcgtgagc tgggtgtctc gtttctacca
     4021 gctgaagcct gtcggtactg cttcttcagc taggggtagg ctgggcctgc gccgttgatg
     4081 agaattcaaa ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga atctagagcc
     4141 tgcagtctcg aggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
     4201 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4261 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     4441 gagcctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     4501 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     4561 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     4621 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     4681 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
     4741 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga
     4801 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4861 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4921 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4981 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5041 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
     5101 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5161 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5221 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5281 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5341 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5401 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5461 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5521 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5581 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5641 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5701 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5761 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5821 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5881 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5941 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6001 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6061 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaa
//
