LOCUS       sequence-454178-       17201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for 
            dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was 
            driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17201)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 17201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT14
FEATURES             Location/Qualifiers
     source          1..17201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3273..3338
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3345..3365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3390..3413,3414..3416,3417..7490)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7403..7411
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7491..7538
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7560..7808
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7821..7841
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7863..8115
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8160..8180
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(8541..8846)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     8711..8727
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8737..8756
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8737..8755
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8803..8823)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8803..8821)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8842..8858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8866..8882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8890..8896,8897..8914,8915..8920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8935..8956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        8998..9073
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9276..9300)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9368..9384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9392..9408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9416..9422,9423..9440,9441..9446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9461..9482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9673..10349
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10286..10306
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10394..10945
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    10952..11126
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11204..11228
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11653..12447
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12534..13122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13023..13042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13276..13293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13308..13448
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13374..13393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13534..13556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13792..13986
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14052..15125)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15554..16183)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     1381 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 acccgtcggc acctccgctt caaggtacgc cgctcgtccy cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag caggctccga attcgccctt catggaggcc tctccggctt
     2641 caggtcctcg ccatctcatg gacccccata tctttacctc caatttcaat aacggcattg
     2701 gcaggcacaa gacatacctc tgttatgagg tcgagcggct cgataacgga acctcagtga
     2761 agatggacca acacaggggt ttccttcata accaagcgaa aaatctcctt tgcggtttct
     2821 atgggcggca cgcggagctg agattcttgg atttggtccc gagcctccag ctggatcccg
     2881 cgcaaatata ccgcgtcact tggttcatat catggagccc gtgctttagc tgggggtgtg
     2941 ccggtgaggt tcgcgcgttc ctccaggaaa acacccatgt tcgcctccgg attttcgcag
     3001 cgaggatttt cgactatgat ccactttaca aagaggccct ccaaatgctt agagatgctg
     3061 gggctcaagt ttcaattatg acatacgacg agtttaagca ttgttgggac acttttgtcg
     3121 atcatcaggg ttgtcccttc cagccgtggg atggtcttga tgagcattcg caagctttga
     3181 gtggcaggct tcgcgcgata cttcagaacc agggtaactc aggttctgag acgcctggca
     3241 caagtgagtc agcaacaccc gagtccacca tggattacaa ggaccacgac ggggattaca
     3301 aggaccacga cattgattac aaggatgatg atgacaagat ggctccgaag aagaagagga
     3361 aggttggcat ccacggggtg ccagctgctg acaagaagta ctcgatcggc ctcgctattg
     3421 ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt
     3481 tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc
     3541 tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt
     3601 acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga
     3661 aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga
     3721 agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt
     3781 accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc
     3841 gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg
     3901 gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt
     3961 acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc
     4021 tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg
     4081 agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact
     4141 tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg
     4201 acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg
     4261 ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga
     4321 ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc
     4381 tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct
     4441 tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt
     4501 tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga
     4561 agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac
     4621 accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt
     4681 tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg
     4741 tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga
     4801 cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca
     4861 tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact
     4921 cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg
     4981 agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc
     5041 tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga
     5101 ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg
     5161 ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga
     5221 atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga
     5281 tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca
     5341 agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg
     5401 acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca
     5461 atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc
     5521 aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag
     5581 ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg
     5641 gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga
     5701 agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg
     5761 ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct
     5821 acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca
     5881 ggctcagcga ttacgacgtc gatcatatcg ttccacagtc attcctgaag gatgactcca
     5941 ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt
     6001 cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga
     6061 tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg
     6121 acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg
     6181 cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg
     6241 aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt
     6301 tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg
     6361 tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg
     6421 actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg
     6481 ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc
     6541 tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga
     6601 tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag
     6661 tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattctgc
     6721 ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg
     6781 gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg
     6841 gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct
     6901 ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga
     6961 aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc
     7021 ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg ccgtccaagt
     7081 atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca
     7141 acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc
     7201 agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct
     7261 ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc
     7321 tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac acaactatcg
     7381 atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc caccagtcga
     7441 ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac aagcggccag
     7501 cggcgacgaa gaaggcgggg caggcgaaga agaagaaggg agacggatca ggcgggtcaa
     7561 caaacctcag tgacatcata gagaaggaaa ctggtaagca actggttatt caagagagca
     7621 ttctgatgct ccctgaggaa gtcgaggaag ttataggaaa caagcctgag agcgatatac
     7681 tcgtgcatac ggcgtatgac gagagcacgg atgaaaatgt gatgctcttg accagcgatg
     7741 cgccagaata taaaccctgg gcactggtta ttcaggactc taacggggaa aataaaatca
     7801 agatgctctc aggtggctcc ccaaagaaga aacgcaaggt ttagtggcgg ccgccgacgt
     7861 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     7921 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     7981 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     8041 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     8101 ctatgttact agatcgggaa ttgatccccc ctcgacagct tccggaaagg gcgaattcgc
     8161 aactttgtat acaaaagttg ccccatggcg ttccctctag ataacgcagg atcctattta
     8221 ctttaaattt ttcttatggc tcagcctgtg atggataact gaatcaaaca aatggcgtct
     8281 gggtttaaga acatctgttt tggctatgtt ggacgaaaca agtgaacttt taggatcaac
     8341 ttccgtttat atacggagct tatatcgagc aataagataa gtgggctttt tatgtaattt
     8401 aatgggctat cgtccatata ttcactaata cccatgccca gtacccatgt atgcgtttca
     8461 tataagctcc taatttctcc cacatcgctc aaatctaaac aaatcttgtt gtatatataa
     8521 cactgaggga gcaccattgg tcagagaccg gcgccgctac agggcgcgtc ccattcgcca
     8581 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8641 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8701 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     8761 ttgggtaccg ggccccccct cgaggtcctc cagcttttgt tccctttagt gagggttaat
     8821 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8881 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8941 gagctaactc acattaattg cgttgcgctc actgcccgct ttccaccggt ggtctctgtt
     9001 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     9061 accgagtcgg tgcttttttt tccctttcct tttttctttt ttttgccata aacttaaatt
     9121 tgtatatcga tcattgtaga tattgaaaac ctagaacaaa ccaacatcca tgtgaatgtc
     9181 tttcatgact gatttagaga taattcttga attttggaac tagaatctat aatgagccta
     9241 aattaaaaca ttgtgaacta gtaagggcga attcgaccca gctttcttgt acaaagtggt
     9301 tcgataattc ttaattaact agttctagag cggccgccac cgcggtggag ctcgaattcg
     9361 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9421 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa
     9601 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     9661 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     9721 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     9781 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
     9841 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     9901 ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc tactccaaga
     9961 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
    10021 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
    10081 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
    10141 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    10201 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
    10261 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
    10321 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca aatctatctc
    10381 tctcgagtct accatgagcc cagaacgacg cccggccgac atccgccgtg ccaccgaggc
    10441 ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca cggtcaactt
    10501 ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg
    10561 ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc
    10621 ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg tctccccccg
    10681 ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt ccctggaggc
    10741 acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat
    10801 gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct tcaagcacgg
    10861 gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtac cgccccgtcc
    10921 ggtcctgccc gtcaccgaga tttgactcga gtttctccat aataatgtgt gagtagttcc
    10981 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
    11041 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
    11101 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
    11161 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    11221 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
    11281 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
    11341 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
    11401 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
    11461 tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
    11521 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
    11581 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga
    11641 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
    11701 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
    11761 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    11821 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    11881 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    11941 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
    12001 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    12061 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    12121 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    12181 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    12241 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    12301 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag
    12361 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    12421 tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat cccttaacgt
    12481 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    12541 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    12601 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    12661 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    12721 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    12781 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    12841 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    12901 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    12961 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    13021 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    13081 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    13141 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    13201 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    13261 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
    13321 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
    13381 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
    13441 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    13501 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    13561 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
    13621 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
    13681 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
    13741 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
    13801 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
    13861 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
    13921 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
    13981 tccacaggaa acagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
    14041 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
    14101 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
    14161 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
    14221 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
    14281 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
    14341 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
    14401 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
    14461 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
    14521 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
    14581 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
    14641 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
    14701 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
    14761 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
    14821 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
    14881 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
    14941 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
    15001 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
    15061 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
    15121 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg caggcaaccc
    15181 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
    15241 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
    15301 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
    15361 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
    15421 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
    15481 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
    15541 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
    15601 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
    15661 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
    15721 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
    15781 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
    15841 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
    15901 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
    15961 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
    16021 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
    16081 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
    16141 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
    16201 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
    16261 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
    16321 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
    16381 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
    16441 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
    16501 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
    16561 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
    16621 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
    16681 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
    16741 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
    16801 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
    16861 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
    16921 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
    16981 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
    17041 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
    17101 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
    17161 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat t
//
