LOCUS       sequence-454180-       17822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for 
            dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 
            was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17822)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 17822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT19
FEATURES             Location/Qualifiers
     source          1..17822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1125..1319
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1555..1577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1663..1803
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1718..1737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1818..1835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1989..2577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2069..2088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2664..3458)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5082..5106
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5184..5358
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5399..6424)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6491..7167)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6534..6554)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7358..7379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7394..7399,7400..7417,7418..7424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7432..7448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7456..7472
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(7489..7741)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7805..7829
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(8032..8107)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8149..8170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8185..8190,8191..8208,8209..8215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8223..8239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8247..8263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8259..8564
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     8282..8302
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8284..8302
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8349..8368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8350..8368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8378..8394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8925..8945)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(8990..9242)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9264..9284)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9297..9545)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(9567..9614)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             9694..9702
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(13740..13760)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13767..13832)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(14499..14523)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(15206..15222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    15425..15449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16750..17379
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(17808..17822,1..1059)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
       61 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
      121 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
      181 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
      241 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
      301 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
      361 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
      421 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
      481 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
      541 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
      601 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
      661 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
      721 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
      781 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
      841 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
      901 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
      961 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     1021 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     1081 caaattgccc tagcagggga aaaaggtcga aaaggtctgt ttcctgtgga tagcacgtac
     1141 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     1201 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     1261 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     1321 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     1381 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     1441 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     1501 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     1561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     1621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     1681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     1741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2641 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
     2701 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     2761 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     2821 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     2881 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     2941 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     3001 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     3061 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
     3121 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
     3181 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
     3241 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
     3301 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
     3361 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
     3421 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
     3481 tacagtattt aaagataccc caagaagcta aggaaggtgc gaataagcgg ggaaattctt
     3541 ctcggctgac tcagtcattt catttcttca tgtttgagcc gattttttct cccgtaaatg
     3601 ccttgaatca gcctatttag accgtttctt cgccatttaa ggcgttatcc ccagttttta
     3661 gtgagatctc tcccactgac gtatcatttg gtccgcccga aacaggttgg ccagcgtgaa
     3721 taacatcgcc agttggttat cgtttttcag caaccccttg tatctggctt tcacgaagcc
     3781 gaactgtcgc ttgatgatgc gaaatgggtg ctccaccctg gcccggatgc tggctttcat
     3841 gtattcgatg ttgatggccg ttttgttctt gcgtggatgc tgtttcaagg ttcttacctt
     3901 gccggggcgc tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg
     3961 cgccccttgg tagccggcat cggctgagac aaattgctcc tctccatgca gcagattacc
     4021 cagctgattg aggtcatgct cgttggccgc ggtggtgacc aggctgtggg tcaggccact
     4081 cttggcatcg acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt
     4141 ctgatgcatc tccggatcgc gttgctgctc tttgttcttg gtcgagctgg gtgcctcaat
     4201 gatggtggca tcgaccaagg tgccttgagt catcatgacg cctgcttcgg ccagccagcg
     4261 attgatggtc ttgaacaatt ggcgggccag ttgatgctgc tccagcaggt ggcggaaatt
     4321 catgatggtg gtgcggtccg gcaaggcgct atccagggat aaccgggcaa acagacgcat
     4381 ggaggcgatt tcgtacagag catcttccat cgcgccatcg ctcaggttgt accaatgctg
     4441 catgcagtga atgcgtagca tggtttccag cggataaggt cgccggccat taccagcctt
     4501 ggggtaaaac ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg
     4561 ggacaagaaa atctcttttc tggtctgacg gcgcttactg ctgaattcac tgtcggcgaa
     4621 ggtaagttga tgactcatga tgaaccctgt tctatggctc cagatgacaa acatgatctc
     4681 atatcaggga cttgttcgca ccttccctaa ttataacaag acgaactcca attcactgtt
     4741 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     4801 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     4861 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     4921 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     4981 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     5041 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     5101 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     5161 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     5221 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     5281 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     5341 cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct
     5401 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     5461 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     5521 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     5581 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     5641 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     5701 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     5761 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     5821 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     5881 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     5941 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     6001 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     6061 tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt
     6121 cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc tcgctaaact
     6181 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     6241 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     6301 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     6361 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     6421 tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag
     6481 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     6541 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     6601 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     6661 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6721 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6781 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6841 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6901 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     6961 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     7021 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     7081 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     7141 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     7201 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     7261 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7321 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7381 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7441 gataacaatt tcacacagga aacagctatg acatgattac gaattcccga tctagtaaca
     7501 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
     7561 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
     7621 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
     7681 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
     7741 cggggaaatt cgagctccac cgcggtggcg gccgctctag aactagttaa ttaagaatta
     7801 tcgaaccact ttgtacaaga aagctgggtc gaattcgccc ttactagttc acaatgtttt
     7861 aatttaggct cattatagat tctagttcca aaattcaaga attatctcta aatcagtcat
     7921 gaaagacatt cacatggatg ttggtttgtt ctaggttttc aatatctaca atgatcgata
     7981 tacaaattta agtttatggc aaaaaaaaga aaaaaggaaa gggaaaaaaa agcaccgact
     8041 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
     8101 ctaaaacaga gaccaccggt ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8161 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8221 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8281 cgcaattaac cctcactaaa gggaacaaaa gctggaggac ctcgaggggg ggcccggtac
     8341 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     8401 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8461 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8521 tgaatggcga atgggacgcg ccctgtagcg gcgccggtct ctgaccaatg gtgctccctc
     8581 agtgttatat atacaacaag atttgtttag atttgagcga tgtgggagaa attaggagct
     8641 tatatgaaac gcatacatgg gtactgggca tgggtattag tgaatatatg gacgatagcc
     8701 cattaaatta cataaaaagc ccacttatct tattgctcga tataagctcc gtatataaac
     8761 ggaagttgat cctaaaagtt cacttgtttc gtccaacata gccaaaacag atgttcttaa
     8821 acccagacgc catttgtttg attcagttat ccatcacagg ctgagccata agaaaaattt
     8881 aaagtaaata ggatcctgcg ttatctagag ggaacgccat ggggcaactt ttgtatacaa
     8941 agttgcgaat tcgccctttc cggaagctgt cgagggggga tcaattcccg atctagtaac
     9001 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     9061 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     9121 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     9181 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     9241 tcggacgtcg gcggccgcca ctaaaccttg cgtttcttct ttggggagcc acctgagagc
     9301 atcttgattt tattttcccc gttagagtcc tgaataacca gtgcccaggg tttatattct
     9361 ggcgcatcgc tggtcaagag catcacattt tcatccgtgc tctcgtcata cgccgtatgc
     9421 acgagtatat cgctctcagg cttgtttcct ataacttcct cgacttcctc agggagcatc
     9481 agaatgctct cttgaataac cagttgctta ccagtttcct tctctatgat gtcactgagg
     9541 tttgttgacc cgcctgatcc gtctcccttc ttcttcttcg cctgccccgc cttcttcgtc
     9601 gccgctggcc gcttgtcgcc cccgagctga gacaggtcga tgcgcgtctc gtagaggccg
     9661 gtaatcgact ggtggatgag ggtcgcgtcc aggacctcct tagtgctcct gtactgcttc
     9721 ggatcgatag ttgtgtcgaa gtacttgaaa gccctagggg cgccgagcct cgtcagggtg
     9781 aagagatgaa tgatattctc agcctgctcc ctgattggct tgtcgcggtg cttgttgtac
     9841 gcggagagga ccttatccag attcgcgtcg gccaggatca cgcgcttgga gaactcggaa
     9901 atctgctcaa tgatctcgtc gaggtaatgc ttgtgctgct cgacgaacag ctgcttctgc
     9961 tcgttgtcct cggggctgcc cttgagcttc tcgtagtggg aggccaggta gaggaagttc
    10021 acatacttgg acggcagagc cagctcgttc cccttctgca gctgcttagc ggaagccagc
    10081 atccgcttcc tgccgttctc cagctcgaag agtgagtact tggggagctt aatgatcagg
    10141 tccttcttca cctccttgta gcccttcgcc tccaggaaat cgatcgggtt cttctcgaag
    10201 ctggagcgct ccataatcgt gatccccagc agctccttca cgctcttgag cttcttggac
    10261 ttgcccttct caaccttcgc cacaaccagg accgagtagg ccacagtggg ccacaggaac
    10321 ccgccgtact tcttcggatc ccagtccttc ttgcgggcga tgagcttgtc gctgttccgc
    10381 ttaggcctaa ttgactcctt agagaacccg ccagtctgga cctctgtctt cttgacgata
    10441 ttcacttgtg gcatggagag aaccttcctg acggtcgcga aatccctgcc cttgtcccac
    10501 acgatctccc ccgtctcgcc gttcgtctcg atgagggggc gcttccggat ctcgccattg
    10561 gccagagtga tctctgtctt gaagaaattc ataatgttag agtagaagaa gtacttggcg
    10621 gtagccttgc caatctcctg ctccgacttg gcgatcatct tcctcacatc gtaaaccttg
    10681 tagtccccgt acacgaactc gctctcgagc tttgggtact tcttgatcag agctgtgccg
    10741 accaccgcgt tcaggtacgc gtcatgggca tggtggtaat tgttgatctc ccgaaccttg
    10801 tagaactgga aatccttcct gaagtcggag acgagctttg acttcagggt gatgaccttc
    10861 acctcgcgga tcagcttgtc attctcatcg tacttagtgt tcatccgtga gtcgagaatc
    10921 tgcgcaacgt gcttagtgat ctgccgtgtc tcgaccagct gcctcttgat gaagcccgcc
    10981 ttgtccagct cagagagccc gcccctctca gcctttgtga ggttatcgaa cttccgctgc
    11041 gtgatcagct tggcattcag gagctggcgc cagtagttct tcatcttctt aacgacctcc
    11101 tctgaaggaa cattatcaga cttgccccgg ttcttgtccg acctggtgag gaccttgttg
    11161 tcaatggagt catccttcag gaatgactgt ggaacgatat gatcgacgtc gtaatcgctg
    11221 agcctgttaa tatccagctc ctggtccaca tacatatcgc ggccattctg gaggtagtac
    11281 aggtagagct tctcattctg cagctgcgtg ttctccaccg ggtgctcctt gaggatctgg
    11341 gaccccagct ccttaatgcc ctcctcgatc ctcttcatcc tctcgcgtga gttcttctgg
    11401 cccttctgcg tggtctgatt ctcccgggcc atctcaatga cgatgttctc aggcttgtgc
    11461 ctgcccatga ccttcaccag ctcgtccaca accttcacgg tctgcagaat ccccttcttg
    11521 atagctggcg agccagcgag gttcgcgata tgctcgtgca gcgagtcccc ctggccgctc
    11581 acctgagcct tctggatatc ctccttgaat gtgaggctgt catcgtgaat cagctgcatg
    11641 aaattgcggt tcgcgaagcc atcgctcttc aggaagtcga ggatcgtctt cccggactgc
    11701 ttgtcccgaa tgccgttgat gagcttcctg ctcagcctcc cccagccggt gtacctcctc
    11761 ctcttgagct gcttcatgac cttgtcatcg aagagatggg cgtaagtctt caggcgctcc
    11821 tcgatcatct cccggtcctc gaacagagtg agtgtcagca caatgtcctc gaggatatcc
    11881 tcattctcct cgttgtccag gaagtcctta tccttaatga tcttcaggag atcgtggtag
    11941 gtccccaggg aggcgttgaa gcggtcctca acgccagaga tctcgaccga atcgaagcac
    12001 tcaatcttct tgaagtagtc ctccttgagc tgcttaaccg tgaccttccg gttggtcttg
    12061 aacaggaggt ccacgatggc cttcttctgc tccccagaca ggaaagccgg cttcctcatg
    12121 ccctcggtca catacttcac cttagtcagc tcgttgtaga ctgtgaagta ctcgtacagg
    12181 agcgagtgct tagggagcac cttctcattt ggcaggttct tgtcgaaatt cgtcatcctc
    12241 tcgatgaacg actgagcgct agcgcccttg tcgaccacct cctcgaagtt ccacggcgtg
    12301 atcgtctcct ctgacttgcg ggtcatccaa gcgaagcggg agttgcccct agcgagtggg
    12361 ccgacgtagt acgggatcct gaaagtcaga atcttctcga tcttctcgcg gttatccttg
    12421 aggaaagggt agaagtcctc ctgcctcctc aggatagcgt gcagctcccc gagatgaatc
    12481 tggtgtggga tgctgccgtt atcgaatgtc cgctgcttcc tcaggaggtc ctcgcgattg
    12541 agcttcacca gcagctcctc cgtgccgtcc atcttctcca gaatcggctt gatgaacttg
    12601 tagaactcct cctgagaggc cccgccgtca atgtacccag cgtagccgtt cttcgactga
    12661 tcgaagaaga tctccttgta cttctcgggg agctgctgcc tgaccagcgc cttcaggagg
    12721 gtcagatcct gatggtgctc gtcgtagcgc ttgatcatgg aggctgagag cggagccttc
    12781 gtaatctcgg tgttcaccct gagaatatca gacaggagga tggcgtccga cagattcttg
    12841 gcagcgagga acaggtccgc gtactgatcg ccgatctggg ccaggaggtt atccaggtca
    12901 tcgtcgtatg tgtccttgga gagctgcagc ttggcgtcct cagcgagatc gaaattcgac
    12961 ttgaagttgg gcgtgagccc caggctgagc gcaatgagat tcccgaacag gccgttcttc
    13021 ttctcgcccg gcagctgggc gatcaggttc tcgaggcgcc gagacttcga gagcctagcg
    13081 gacaggatag ccttcgcgtc gacgcctgac gcattaatgg ggttctcctc gaagagctgg
    13141 ttgtacgtct gcacgagctg gatgaacagc ttgtcaacat cgctattgtc cgggttgaga
    13201 tccccctcga tcaggaaatg gcccctgaac ttaatcatgt gggccagagc gaggtagatc
    13261 aggcggaggt ccgccttatc tgtggagtcc acgagcttct tccgcagatg gtagatcgta
    13321 gggtacttct cgtggtaggc aacctcgtcg acaatgttgc cgaagattgg atgccgctcg
    13381 tgcttcttat cctcctccac gaggaatgac tcctccagcc tgtggaagaa agaatcgtca
    13441 accttcgcca tctcgttgga gaaaatctcc tgcaggtagc agatgcgatt cttcctgcgc
    13501 gtgtaccgcc tgcgggcggt gcgcttgagc cgcgtcctct cagccgtctc gccgctgtcg
    13561 aacaggagag cgccaatgag attcttcttg atggaatgcc gatcggtgtt gcccaggacc
    13621 ttgaacttct ttgagggcac cttgtactcg tcggtgatca cggcccagcc aacagagtta
    13681 gtcccaatag cgaggccgat cgagtacttc ttgtcagcag ctggcacccc gtggatgcca
    13741 accttcctct tcttcttcgg agccatcttg tcatcatcat ccttgtaatc aatgtcgtgg
    13801 tccttgtaat ccccgtcgtg gtccttgtaa tccatggtgg actcgggtgt tgctgactca
    13861 cttgtgccag gcgtctcaga acctgagtta ccctggttct gaagtatcgc gcgaagcctg
    13921 ccactcaaag cttgcgaatg ctcatcaaga ccatcccacg gctggaaggg acaaccctga
    13981 tgatcgacaa aagtgtccca acaatgctta aactcgtcgt atgtcataat tgaaacttga
    14041 gccccagcat ctctaagcat ttggagggcc tctttgtaaa gtggatcata gtcgaaaatc
    14101 ctcgctgcga aaatccggag gcgaacatgg gtgttttcct ggaggaacgc gcgaacctca
    14161 ccggcacacc cccagctaaa gcacgggctc catgatatga accaagtgac gcggtatatt
    14221 tgcgcgggat ccagctggag gctcgggacc aaatccaaga atctcagctc cgcgtgccgc
    14281 ccatagaaac cgcaaaggag atttttcgct tggttatgaa ggaaacccct gtgttggtcc
    14341 atcttcactg aggttccgtt atcgagccgc tcgacctcat aacagaggta tgtcttgtgc
    14401 ctgccaatgc cgttattgaa attggaggta aagatatggg ggtccatgag atggcgagga
    14461 cctgaagccg gagaggcctc catgaagggc gaattcggag cctgcttttt tgtacaaact
    14521 tgtttgatag cttggcgcgc ctcgaggggg ggcccggtac cctgttaatc agaaaaactc
    14581 agattaatct acaaattcga tcgcacaaac tagaaactaa caccagatct agatagaaat
    14641 cacaaatcga agagtaatta ttcgacaaaa ctcaaattat ttgaacaaat cggatgatat
    14701 ctatgaaacc ctaatcgaga attaagatga tatctaacga tcaaacccag aaaatcgtct
    14761 tcgatctaag attaacagaa tctaaaccaa agaacatata cgaaattggg atcgaacgaa
    14821 aacaaaatcg aagattttga gagaataagg aacacagaaa tttaccttga tcacggtaga
    14881 gagaattgag agaaagtttt taagattttg agaaattgaa atctgaattg tgaagaagaa
    14941 gagctctttg ggtattgttt tatagaagaa gaagaagaaa agacgaggac gactaggtca
    15001 cgagaaagct aaggcggtga agcaatagct aataataaaa tgacacgtgt attgagcgtt
    15061 gtttacacgc aaagttgttt ttggctaatt gccttatttt taggttgagg aaaagtattt
    15121 gtgctttgag ttgataaaca cgactcgtgt gtgccggctg caaccacttt gacgccgttt
    15181 attactgact cgtcgaaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    15241 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    15301 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    15361 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
    15421 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
    15481 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
    15541 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
    15601 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
    15661 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
    15721 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
    15781 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
    15841 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
    15901 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
    15961 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
    16021 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
    16081 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
    16141 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
    16201 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
    16261 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
    16321 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
    16381 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
    16441 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
    16501 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
    16561 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
    16621 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    16681 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    16741 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    16801 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    16861 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    16921 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    16981 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    17041 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    17101 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    17161 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    17221 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    17281 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    17341 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    17401 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    17461 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    17521 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    17581 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    17641 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    17701 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
    17761 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
    17821 aa
//
