LOCUS       sequence-454202-        6417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for MLV Gag with truncated MA domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pGag∆MA
FEATURES             Location/Qualifiers
     source          1..6417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          799..1274
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             2292..2348
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2349..3047
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR
                     TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF
                     KTPDADAGEE"
     primer_bind     complement(3066..3083)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3072..3279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3423..3817
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(4149..4167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4235..4339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4340..4408,4409..5200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4558..4577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5736..5755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt accgagctcg
      781 gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc cccttctttt
      841 ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat attgaccaaa
      901 tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt
      961 gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat
     1021 gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa
     1081 tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt
     1141 catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat
     1201 aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta
     1261 tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca
     1321 aagaattcgc caccatgccc ctccgcgcag gaggaaacgg acagcttcaa tactggccgt
     1381 tctcctcttc tgacctttac aactggaaaa ataataaccc ttctttttct gaagatccag
     1441 gtaaactgac agctctgatc gagtctgtcc tcatcaccca tcagcccacc tgggacgact
     1501 gtcagcagct gttggggact ctgctgaccg gagaagaaaa acaacgggtg ctcttagagg
     1561 ctagaaaggc ggtgcggggc gatgatgggc gccccactca actgcccaat gaagtcgatg
     1621 ccgcttttcc cctcgagcgc ccagactggg attacaccac ccaggcaggt aggaaccacc
     1681 tagtccacta tcgccagttg ctcctagcgg gtctccaaaa cgcgggcaga agccccacca
     1741 atttggccaa ggtaaaagga ataacacaag ggcccaatga gtctccctcg gccttcctag
     1801 agagacttaa ggaagcctat cgcaggtaca ctccttatga ccctgaggac ccagggcaag
     1861 aaactaatgt gtctatgtct ttcatttggc agtctgcccc agacattggg agaaagttag
     1921 agaggttaga agatttaaaa aacaagacgc ttggagattt ggttagagag gcagaaaaga
     1981 tctttaataa acgagaaacc ccggaagaaa gagaggaacg tatcaggaga gaaacagagg
     2041 aaaaagaaga acgccgtagg acagaggatg agcagaaaga gaaagaaaga gatcgtagga
     2101 gacatagaga gatgagcaag ctattggcca ctgtcgttag tggacagaaa caggatagac
     2161 agggaggaga acgaaggagg tcccaactcg atcgcgacca gtgtgcctac tgcaaagaaa
     2221 aggggcactg ggctaaagat tgtcccaaga aaccacgagg acctcgggga ccaagaccgc
     2281 agggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc
     2341 ctggaccgat ggagagcgac gagagcggcc tgcccgccat gaagatcgag tgccgcatca
     2401 ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccgagc
     2461 agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc
     2521 tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg
     2581 agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt
     2641 acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga
     2701 tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca
     2761 agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg
     2821 tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg
     2881 acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca
     2941 tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt
     3001 accagcacgc cttcaagacc ccggatgcag atgccggtga agaatagcta gagctcgctg
     3061 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3121 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3181 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3241 gggggaggat tgggaagaga atagcaggca tgctggggac atggcgcgtt caacgctctc
     3301 aaaacccctt aaaaataagg ttaacccgcg aggcccccta agaattcacc ccaccagtgc
     3361 aggctgccta tcagaaagtg gtggctggtg tggctaatgc cctggcccac aagtatcact
     3421 aagctcgctt tcttgctgtc caatttctat taaaggttcc tttgttccct aagtccaact
     3481 actaaactgg gggatattat gaagggcctt gagcatctgg attctgccta ataaaaaaca
     3541 tttattttca ttgcaatgat gtatttaaat tatttctgaa tattttacta aaaagggaat
     3601 gtgggaggtc agtgcattta aaacataaag aaatgaagag ctagttcaaa ccttgggaaa
     3661 atacactata tcttaaactc catgaaagaa ggtgaggctg caaacagcta atgcacattg
     3721 gcaacagccc ctgatgccta tgccttattc atccctcaga aaaggattca agtagaggct
     3781 tgatttggag gttaaagttt tgctatgctg tattttacat tacttattgt tttagctgtc
     3841 ctcatgaatg tcttttcact acccatttgc ttatcctgca tctctcagcc ttgactccac
     3901 tcagttctct tgcttagaga taccaccttt cccctgaagt gttccttcca tgttttacgg
     3961 cgagatggtt tctcctcgcc tggccactca gccttagttg tctctgttgt cttatagagg
     4021 tctacttgaa gaaggaaaaa cagggggcat ggtttgactg tcctgtgagc ccttcttccc
     4081 tgcctccccc actcacagtg acccggaatc cctcgacatg gcagtctaga attcttgaag
     4141 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4201 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4561 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4861 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5821 cggagcctat ggaaaaacgc cagcaacgga gatgcgccgc gtgcggctgc tggagatggc
     5881 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     5941 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     6001 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     6061 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     6121 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     6181 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     6241 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     6301 ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6361 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatg
//
