LOCUS       sequence-454220-        2583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hIAPP-T7n.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Yang L, Zhang J, Andon JS, Li L, Wang T
  TITLE     Rapid discovery of cyclic peptide protein aggregation inhibitors by 
            continuous selection.
  JOURNAL   Nat Chem Biol. 2025 Jan 13. doi: 10.1038/s41589-024-01823-x.
  PUBMED    39806068
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 13. doi: 10.1038/s41589-024-01823-x."
COMMENT     Sequence Label: pTW358f
FEATURES             Location/Qualifiers
     source          1..2583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(72..617)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(152..171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1699..2514)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1699..2514)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2422..2441
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2506..2525
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagat attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 gggaccctga tgaaattcgc cacttgttag tgtaattcgc taactcatcc tggcatgttg
      781 ctgttgattc ttcaatcaga tctttataaa tcaaaaagat aaaaaattgg cacgcaaatt
      841 gtattaacag ttcagcagga caatcctgaa cgcagaaatc aagaggacaa cattacctgc
      901 aggtgcagta aggaggaaaa aaaaatgaaa tgcaacactg ccacatgtgc aacgcagcgc
      961 ctggcaaatt ttttagttca ttccagcaac aactttggtg ccattctctc atctaccaac
     1021 gtgggatcca atacatatgg tggatctggt ggtaacacga ttaacatcgc taagaacgac
     1081 ttctctgaca tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag
     1141 cgtttagctc gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc
     1201 ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc
     1261 aagcctctca tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag
     1321 gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc agttcctgca agaaatcaag
     1381 ccggaagccg tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac
     1441 aatacaaccg ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc
     1501 ttcggtcgta tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc
     1561 aacaagcgcg tagggcacgt ctacaagtaa attaacggca ctgcgaaaaa accccgccga
     1621 agcggggttt tttgcgaaat ataatgaccc tcttgataac ccaagagggc attttttaat
     1681 gcccatggcg tacggatctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     1741 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     1801 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     1861 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     1921 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     1981 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     2041 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     2101 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     2161 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     2221 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     2281 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     2341 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     2401 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     2461 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     2521 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaagaga aacctacgga
     2581 tcg
//
