LOCUS       sequence-454223-        2598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express A-beta-42-T7n.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Yang L, Zhang J, Andon JS, Li L, Wang T
  TITLE     Rapid discovery of cyclic peptide protein aggregation inhibitors by 
            continuous selection.
  JOURNAL   Nat Chem Biol. 2025 Jan 13. doi: 10.1038/s41589-024-01823-x.
  PUBMED    39806068
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 13. doi: 10.1038/s41589-024-01823-x."
COMMENT     Sequence Label: pTW358b
FEATURES             Location/Qualifiers
     source          1..2598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(72..617)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(152..171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1714..2529)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1714..2529)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2437..2456
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2521..2540
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagat attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 gggaccctga tgaaattcgc cacttgttag tgtaattcgc taactcatcc tggcatgttg
      781 ctgttgattc ttcaatcaga tctttataaa tcaaaaagat aaaaaattgg cacgcaaatt
      841 gtattaacag ttcagcagga caatcctgaa cgcagaaatc aagaggacaa cattacctgc
      901 aggtgcagta aggaggaaaa aaaaatggac gcagagttcc gtcacgacag tggctatgaa
      961 gtccaccacc agaaattggt ttttttcgcc gaggatgttg gtagcaataa aggggctatc
     1021 atcggtttaa tggtaggtgg agttgttatt gcaggtggat ctggtggtaa cacgattaac
     1081 atcgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa cactctggct
     1141 gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga gtcttacgag
     1201 atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg
     1261 gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat tgcacgcatc
     1321 aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc cttccagttc
     1381 ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac tctggcttgc
     1441 ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt
     1501 gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt caagaaaaac
     1561 gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agtaaattaa cggcactgcg
     1621 aaaaaacccc gccgaagcgg ggttttttgc gaaatataat gaccctcttg ataacccaag
     1681 agggcatttt ttaatgccca tggcgtacgg atcttagaaa aactcatcga gcatcaaatg
     1741 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     1801 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     1861 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     1921 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     1981 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     2041 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     2101 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     2161 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     2221 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     2281 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     2341 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     2401 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     2461 taaatcagca tccatgttgg aatttaatct cggcctagag caagacgttt cccgttgaat
     2521 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     2581 agagaaacct acggatcg
//
