LOCUS       sequence-454226-        4377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 exon 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_exon 4
FEATURES             Location/Qualifiers
     source          1..4377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        170..201
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      228..237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             234..1886
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1930..2051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1941..1960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1995..2014
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2299..2316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2470..3058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2550..2569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3258..4049,4050..4118))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4223..4271
                     /note="synthetic polyadenylation signal"
     misc_feature    4285..4376
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4325..4344
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gcctaactgg ccggtaccca tcagtaatct ccatctcata gggggaaggt ctcctcttca
       61 ctcgattgtt ggtggtgaac atagggaagg catccggctc cccaaagaac ccggagctcg
      121 ctagcctcga ggatatcaag atctggcctc ggcggccaag cttagacact agagggtata
      181 taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accatggaag
      241 atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg
      301 gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta
      361 ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc
      421 tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg
      481 agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg
      541 ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc
      601 ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc
      661 taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa
      721 gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc
      781 ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg
      841 gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc
      901 gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat
      961 ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg
     1021 tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc
     1081 aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca
     1141 agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg
     1201 taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga
     1261 cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag
     1321 gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg
     1381 gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta
     1441 acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca
     1501 tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca
     1561 aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg caacacccca
     1621 acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg
     1681 cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg
     1741 ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc
     1801 ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca
     1861 agaagggcgg caagatcgcc gtgtaataat tctagagtcg gggcggccgg ccgcttcgag
     1921 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1981 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2041 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     2101 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt
     2161 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     2221 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     2281 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2881 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg
     3241 ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc agcgatctgc
     3301 ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac gattcgtgag
     3361 ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc accggccccc
     3421 gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg tcctgctact
     3481 ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca
     3541 gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc acgctcgtcg
     3601 ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac atgatcaccc
     3661 atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag aagtaagttg
     3721 gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac cgtcatgcca
     3781 tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg tgagtagtgt
     3841 atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc gccacatagc
     3901 agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc
     3961 ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg atcttcagca
     4021 tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag
     4081 aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt tcaatattat
     4141 tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa tattaaggta
     4201 cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     4261 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
     4321 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctg
//
