LOCUS       sequence-454227-       11997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of KIF 13a S1371D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Mendoza CS, Plowinske CR, Montgomery AC, Quinones GB, Banker G, 
            Bentley M
  TITLE     Kinesin Regulation in the Proximal Axon is Essential for 
            Dendrite-selective Transport.
  JOURNAL   Mol Biol Cell. 2024 Jun 1;35(6):ar81. doi: 10.1091/mbc.E23-11-0457. 
            Epub 2024 Apr 10.
  PUBMED    38598291
REFERENCE   2  (bases 1 to 11997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-06-1"; volume: "35(6):ar81. doi"; pages: " 
            10.1091/mbc.E23-11-0457. Epub 2024 Apr 10"
COMMENT     Sequence Label: pBa.GFP-KIF13AS1371D
FEATURES             Location/Qualifiers
     source          1..11997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2057..2066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3677..3811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3714..3733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3768..3787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3842..3858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3842..3858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3855..3877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3866..3882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3890..3896,3897..3914,3915..3920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3935..3956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4073..4090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4244..4832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4324..4343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5003..5794,5795..5863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5626..5645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5864..5968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6036..6054
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6092..6114)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6214..6233
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6427..6449
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6441..6458
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6442..6458
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9473..9495
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(9563..9565,9566..9568,9569..10276)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(9608..9629)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9869..9888)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10216..10237
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctagacgct gcgagcgagg cttcctcgga gccagactac aactacgagt tcgcacagat
       61 ggaggtcatc atgaaaacac tgaacagcaa tgatccagtt caaaatgtgg ttcaggtcct
      121 ggagaagcag tacctggaag agaagcggac cgccctggag gaacagcggc tcatgtacga
      181 acgcgagctg gagcagcttc gccagcagct ctctcccgag aggcagccgc ccagcagcgc
      241 ctcagaccgt ctggcctaca gcagccagac agcccagcag aaggtgaccc agtgggccga
      301 ggagagggat gaactcttta ggcagagctt ggccaagctt cgagagcagc tggtgaaagc
      361 caacactttg gtgagggaag ccaacttcct agccgaggag atgagcaaac tcactgacta
      421 ccaagtgact ctgcagatcc ctgcggccaa cctcagtgcc aacaggaaga gaggcgcaat
      481 cgtcagcgag cccgccatcc aagccaggag gaaagggaag ggcacccagg tctggaccat
      541 tgagaagctg gagaataagc taattgatat gagagacctt taccaagaat ggaaagaaaa
      601 tgttcctgag gccaagaggc tctatgggaa gcgaggtgac cctttttatg aagcgcagga
      661 gaatcacaac ctcattggtg tggctaatgt gtttttggaa tgtctcttct gtgatgtgaa
      721 gcttcaatat gcagtaccca tcatcagcca gcagggggag gttgccgggc gcctccatgt
      781 ggaggtgacg cgcattacag gaaccattcc agaacgcatg gcggaggatg actcttcaga
      841 gaactctagt gagagcggga gtcttgaagt ggtcgacagc agcggcgaag tcattcaccg
      901 ggtcaaaaag ctgacatgca gggtgataat taaagaggca accgggctac ccataagcct
      961 ctcaaatttc gtcttctgtc agtacacgtt ctgggaccag tgtgagtcta cagtggctgc
     1021 tccagtggtg gacccagatg tgccctcccc gcagtccaag gacgcacagt acacggtgac
     1081 cttctctcac tgtaaggact acgtggtgac tgtcacagaa gagttcctgg agttcatttc
     1141 tgatggtgcg ctggcgattg aagtgtgggg ccacaggtgt gctgggaacg gaagccccat
     1201 ctgggaggtg gactcgctcc atgcgaagac aaggacattg catgacaggt ggaatgaagt
     1261 gacccggaga atagaggtgt ggatctccat tctggagctg aatgagctcg gggattatgc
     1321 cgctgtggag cttcaccagg cgaaagacgt caacaccggc ggcgtcttcc aactcagaca
     1381 gggtcactcc cgtagagtgc aggtcacagt gaaacctgta cagcactcag ggacactgcc
     1441 actcatggtt gaagccatcc tatcagtatc cattggctgc gtgaccgcca ggtccaccaa
     1501 gctgcagaga gggctggaca gctaccagga agaagacttg aattgtgtaa gagagagatg
     1561 gtccgatgca ctcattaaac gacgggaata cttagacgaa cagattaaaa aagtcagcaa
     1621 taaaaaagag aaaacagaag acgacatgga gcgggaagcc aggctggtgg agcagtgggt
     1681 ggggctgaca gaggagagga atgctgtgct ggtgcccgcc cctggcagcg gaatccccgg
     1741 ggcacctgct gactgggtcc cacctcctgg catggaaaca cacatcccgg ttctcttcct
     1801 ggatctgaac gcggatgacc tcagtgccaa tgagcagctg gtgggccccc atgcgtccgg
     1861 tgtgaactcc atcctgccca aggaacacgg cagtcagttt ttctacctgc ccatcataaa
     1921 gcacagtgat gacgaggttt cagccacagc ctcctgggac tcctctgtgc acgactctct
     1981 tcacctgaat cgggtcacac cgcagaatga aaggatctac ctgatcgtga agaccaccgt
     2041 gcagctcagc cacccggccg ccatggagct agtgttgcgg aagcggatcg cggccaatat
     2101 ttataacaag cagagtttca cccagagttt gaagagacga atatcactga tcaatatacg
     2161 ttactcctgc ggcgtgacct atgagatagt atccaatatc ccaaaggcga ccgaggagat
     2221 tgaggaccgg gaaaccctgg ccctcctggc agcgcggagt gagaacgagg gcactttgga
     2281 tggggagaca tacatcgaga agtacacgcg gggcgtgctg caggtggaga atatcctgag
     2341 cctggagcgg ctccggcagg ctgtcacggt gaaggaagcg ctttccacca aagcgaggca
     2401 cattcgtagg tctctcgata caccgaacgt tcacaacgtc tcctctagcc ggccagatct
     2461 ctctggcttc gatgaagacg ataagggctg gcccgagaac cagttagacg tgtctgacta
     2521 cagctccagc taccaagacg tcgcgtgcta tgggacttta cccagggact cacctcgcag
     2581 gagcaaggaa ggttgtccat cagagaatcc tcatgcctta acagtcagcc cttttaaagc
     2641 gttctctcct cagccaccaa agttcttcaa acccctcatg cctgtaaaag aggagcataa
     2701 gaaaaggttg gccctggaag ccagacctct cctaagccaa gaggactcgg aagaggagga
     2761 aaacgagctg gaagctctca gcaggaagct gatgcttaca cagccttatg tgcccgtgga
     2821 gttcgctgac ttcagtgttt acaatgccag cttggagaac agggagtggt cctcttctaa
     2881 ggcggacctg acagactcga gagccttgga gaaagcagtg tcccgtagcc ctaccaccag
     2941 cagcctcacc agtggctact tctcccacag tgcctccaat gccactctgt ctgacatggc
     3001 agtcccctcc agcgacagct cagaccagct tgccgtctcg acaaaggagg tcgagtgcgc
     3061 cgagcccccg ggaccatcac ttgcaccgga tgtcagacga gcttcgaatc aggagcttac
     3121 agaagtagga agaggctcag ggaaggatga gacaatcgct gtgccgctgg aggaaaacag
     3181 tgccttaccc aaggggaccc cgtcacccca gagcatccct gaggaaagtt ccagaatgcc
     3241 atgcaggact gcttcatgtt cagaactgga tgtcggcccc agcaaagatg gccaccaagc
     3301 cagagaattc tgcccaggag aggtgacgat agaacacact acaaacattt tggaggacca
     3361 ttccttcaca gagttcatgg gtgtttctga tggcaaagac tttgatggtt tggcagattg
     3421 ttctgtaggg gagccctcca ggaggagggc tctaacaaac gaaacagacc ataaaggtat
     3481 cccagaaaga cctccagatg ccgaccggct gcatcccaag atagagaacg accaggaagc
     3541 cacagccact cggtaagcta gataagtaat gatcataatc agccatatca catctgtaga
     3601 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3661 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3721 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3781 actcatcaat gtatcttatc atgtctggat ccccgggtac cgagctcgaa ttcgtaatca
     3841 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     6001 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     6061 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     6121 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     6181 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     6241 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6301 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6361 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6421 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagttggga tctttgcatt
     6481 ggcccacggc tctcaggatg gggatgctcc ccttcagcac ccggttcccc ttggaaactg
     6541 atggtcctgg ctctgtggca tggcagtggc actgtgagga gccccctacc agcagcacac
     6601 agtggggttg gcactgccac gctccggatg ccgcgctctg atccaacccc ataatcaagg
     6661 gaacccgaat tgccccatca ttgcccccac cacccccatc ctgccgggcc ctcacacccc
     6721 acgctgcctt ctggtgacat tccccagccc aaacccacgg cttcatggct accgcggggc
     6781 atttcccatt gccgccccat tatcagctct gcacacctcc cgctgtaccc atgcctcgtg
     6841 gctgcccttc tttgacgtat aatcttctaa ttaatacccg gccttgtcaa agtggagcac
     6901 aaacgttaat taattcccca gcaggcaggt aattaacagt gtgactccct ttttgctgcg
     6961 agtggggctg atacagagag atgtggcact atggagccca cggggtcctg gcactgggtg
     7021 cccacggagg tccccatgtg ctgcagtgtc accgcctccg aggagacagt attgtccctg
     7081 cggtgtccct gcagctcagc tctgtccaca gggccacctc cagtttggag gggacacaat
     7141 gcagccccga tgcaacccat cctcgcagca tcccagggac aaagacccca ctgcaagacc
     7201 gcacacaggg ctgggtcccg ctcccctaat atctacagtg cttcttgcat ggccccttaa
     7261 tcaatgcagt taatcagcat gcgctcatgc accgctctgg agctgcaaag cccctcgcag
     7321 cgctgctcac caacaccgcg caccgccccg gcccagcctg cagcacgcgc tgcaaacagg
     7381 aaagaaacaa aatattgccc aaatgtaggc aaaggcattc ggctgccttg acctccgccg
     7441 ggccgggccc tgcctgactc agctccttac tcagcgctcg cttcctccct ccggctgcca
     7501 ccgccgcagc gcacaccctg acaaagagtg gcccttaacg ggctctgagg tgcacccagc
     7561 agtgcactca gcagtccaag ggccggcctg gaggtttgca ccgctacgtg ctgacattag
     7621 cattgaactt ggccctgggt agtgctgcag gccgggcggg gtgggtgtag agagtgcagc
     7681 gcgcgttgca cccggtgccc cttcccctcc cttgcatccc agcaggctgc accccagcac
     7741 caggcccgtg catgcatgct cctggtgtta ttgcagcctg gtgcatgcat gcgtcttgat
     7801 ggtgcagcgc tgtgcatgca tcctccttgg tgtgtagcag tctagtgcat gcatacccct
     7861 cggtgttatt gctgctctgt gcacgcacgc tcattgtatc acttcatccc agtgcatgca
     7921 ctcacactgg agcgattgct gctcggtgca cgcacactca ttgtatcact gcagctcagt
     7981 ggctgcacgc acaccggtgt tattgctgct cggtgcgtgc atgcacatca gtgtcgctgc
     8041 agctcagtgc atgcacgctc attgcccatc gctatccctg cctctcctgc tggcgctccc
     8101 cgggaggtga cttcaagggg accgcaggac cacctcgggg gtggggggag ggctgcacac
     8161 gcggaccccg ctccccctcc ccaacaaagc actgtggaat caaaaagggg ggagggggga
     8221 tggaggggcg cgtcacaccc ccgccccaca ccctcacctc gaggtgagcc ccacgttctg
     8281 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     8341 attattttgt gcagcgatgg gggcgggggg ggggggcggg cgcccggggg gggggggggg
     8401 gggggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     8461 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     8521 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     8581 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     8641 cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg
     8701 gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg
     8761 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     8821 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     8881 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     8941 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac
     9001 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     9061 cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     9121 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     9181 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     9241 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     9301 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     9361 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctcca tctccagcct
     9421 cggggctgtc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     9481 ttctggcgtg tgaccggcgg ggtttatatc ttcccttctc tgttcctccg cagcccccaa
     9541 gcttggcgcg cctaggccta ccatggtgag caagggcggg gagctgttca ccggggtggt
     9601 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     9661 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     9721 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     9781 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     9841 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     9901 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     9961 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
    10021 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
    10081 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
    10141 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
    10201 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
    10261 catggacgag ctgtacctga cgcgtggagg agctggaata ggcatgtcgg atacgaaggt
    10321 aaaagttgcc gtccgggtcc ggcccatgaa ccgaagagag ctggaactga acaccaagtg
    10381 cgtggtggag atggaaggga accagacggt cctgcaccct cctccttcca acactaagca
    10441 gggagaaaga aaacctccca aggtgtttgc ctttgattat tgcttttggt ccatggatga
    10501 atcgaacaca acaaaatatg ctggtcaaga agtcgttttc aagtgcctcg gggaaggaat
    10561 tctcgagaaa gcctttcagg gatacaatgc ttgtattttt gcatatggcc agacaggttc
    10621 tggaaagtcc ttttccatga tgggccacgc agagcagctg ggccttatcc caaggctctg
    10681 ctgtgctctg tttcaaagga tcgccctgga gcaaaatgag tcacagacct tcaaagtcga
    10741 ggtatcgtat atggagatct ataacgagaa ggtccgggac ctcttggacc ccaaagggag
    10801 caggcagtct ctgaaagtcc gagagcataa ggtcctgggg ccgtatgtcg atggtctgtc
    10861 tcagctggct gtcaccagct ttgaggacat tgagtcactg atgtctgagg gaaataagtc
    10921 tcgaacggta gcagcgacca acatgaatga ggagagcagc cgttcccatg cggtcttcaa
    10981 catcatcatc acacagacgc tgtatgacct acagtctggg aactccgggg agaaggtcag
    11041 caaggtgagc ctggtagacc tggcggggag cgagagagtg tcgaagactg gagctgcggg
    11101 tgagcggctg aaagaaggca gcaacattaa caaatccctt acaaccttgg gattggttat
    11161 atcgtcactg gctgaccagg ctgctggcaa aggcaaaaac aaattcgtgc cttatcgaga
    11221 ttcggtcctt acttggcttc ttaaggacaa tttaggaggg aacagccaga cctctatgat
    11281 agcgaccatc agcccagcag cagacaacta tgaggagaca ctgtccacgc taagatatgc
    11341 agatcgagcc aagaggattg tgaaccacgc tgtggtgaat gaggatccca atgccaaggt
    11401 catccgagag cttcgagagg aagtggagaa actgagggag cagctctcgc aggctgaggc
    11461 catgaaagcc cccgagctaa aggagaagct cgaagagtct gagaagctga taaaggagct
    11521 aacggtgacg tgggaagaga agctgaggaa aacagaagcg atcgcacagg agagacagcg
    11581 gcagctggaa agcatgggaa tctccctgga gacctctggc atcaaggtgg gggacgacaa
    11641 gtgctacctc gtcaacctga acgcagaccc tgccctcaac gagcttctgg tttactattt
    11701 aaaggatcat acccgggtgg gtgcagatac ctctcaggac atccagctct tcggaatagg
    11761 gattcagccc gagcactgtg agatcgacat cgcagcggat ggagacatca ctctcacgcc
    11821 caaagaaaat gcaaggtcct gtggcaatgg cacccttgtc tgcagtacta cccagctgtg
    11881 gcacggcgac aggatcttat ggggaaacaa ccactttttt agaattaact tgcctaagag
    11941 gaaacggcgg gactggttga aagactttga gagagaaacc agttccgcgg agcacga
//
