LOCUS       sequence-454236-       12574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally HA-tagged hBIG1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12574)
  TITLE     Mammalian expression plasmids and entry plasmids for Gateway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG/HA-hBIG1
FEATURES             Location/Qualifiers
     source          1..12574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(22..813,814..882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     645..664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(883..987)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1299..2102
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1362..1381)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1972..1991
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2109..2157
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2167..2546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2826..3842
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3766..3788
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3850..3869
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3911..3937
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    3974..3998
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     mobile_element  6129..6896
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     primer_bind     complement(10423..10439)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10423..10439)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(10461..10485)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10609..10628)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10674..10729
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10728..10747)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11090..11106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11090..11106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11103..11125)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11114..11130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11138..11144,11145..11162,11163..11168))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11183..11204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11263..11458
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11309..11444
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    11464..11598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11501..11520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11555..11574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11666..11683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11837..12425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11917..11936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
       61 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
      241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
      301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
      361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
      421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
      481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
      541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
      601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
      661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
      721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
      781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
      841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
      901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgag
     1021 cagtgtggtt ttcaagagga agcaaaaagc ctctccaccc aggcctggaa tgtttccacc
     1081 caatgtcgag cagtgtggtt ttgcaagagg aagcaaaaag cctctccacc caggcctgga
     1141 atgtttccac ccaatgtcga gcaaaccccg cccagcgtct tgtcattggc gaattaattc
     1201 gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac
     1261 gcgtgtggcc tcgaacaccg agcgaccctg cagccaatat gggatcggcc attgaacaag
     1321 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     1381 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     1441 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     1501 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     1561 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     1621 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     1681 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     1741 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     1801 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg
     1861 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     1921 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     1981 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     2041 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     2101 gaggggatcg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcgga
     2161 tccgtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2221 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2281 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2341 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2401 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2461 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2521 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     2581 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     2641 cagcgatggg ggcggggggg gggggggggg ggcgccaggg gggggggggg gggggggggg
     2701 gggggggggg gggggggggg gggggggggg ggggggccaa aaaaaggggg ggcccccgaa
     2761 gggttctttt tttggggggg gggggggggg gggggcccct aaaaaaagag agggcggggc
     2821 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     2881 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     2941 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     3001 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     3061 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     3121 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     3181 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     3241 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     3301 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     3361 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     3421 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     3481 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     3541 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     3601 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     3661 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     3721 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     3781 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     3841 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatttgcta
     3901 ggccaccatg tacccatacg acgtcccaga ctacgctgga gctcgcggcc gcagatctct
     3961 agttaagcta tcaacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc
     4021 gactggatcc accatgtatg aggggaagaa gacgaagaac atgttcctga cccgggctct
     4081 ggagaagata ttggccgaca aggaagtgaa gaaggcgcat cactcccagc tgcgcaaagc
     4141 ttgcgaggtg gcgttagagg aaataaaagc ggaaactgaa aaacagagtc ctcctcatgg
     4201 agaagcaaaa gctggatcaa gcacccttcc accagtgaaa tcaaagacaa attttattga
     4261 agcagacaag tacttcttgc cttttgagtt ggcatgccag tccaaatgtc ctcgcatagt
     4321 tagtacatct ctagattgct tacagaaact tattgcttat gggcacttga ctggcaatgc
     4381 tccagatagt acaacaccag gcaaaaaatt aattgataga attattgaaa caatatgtgg
     4441 ctgctttcag ggccctcaga cagatgaagg agttcagctg cagataataa aggctttact
     4501 tactgcagta acatcacaac acatagaaat tcatgaaggg actgtactgc aagctgtgag
     4561 aacatgttac aatatctact tagcaagcaa aaatctcatc aatcagacaa cagccaaagc
     4621 tactctcact cagatgctaa atgttatctt tgcacgcatg gaaaaccaag cattacagga
     4681 agcaaaacaa atggaaaaag aaaggcatcg gcagcatcat catctgttac agtctccagt
     4741 aagccatcac gagcctgaat cacctcaact tagatatttg ccacctcaga ctgttgatca
     4801 tatatcccaa gaacacgaag gggaccttga cctccataca aatgatgtag ataaaagtct
     4861 tcaggatgac acagagcctg aaaatggatc tgatatttcc agtgcagaaa atgaacagac
     4921 tgaagctgat caggcaactg cagctgaaac attatctaaa aatgaagtgt tatatgacgg
     4981 agaaaaccat gattgtgagg aaaagccaca agacattgta cagaacattg tagaagaaat
     5041 ggtgaacatt gttgttggag atatgggaga agggactact ataaatgcaa gtgcagatgg
     5101 caacattgga actatagagg atggtagtga cagtgaaaat attcaagcaa atggaattcc
     5161 aggaacacca atttctgttg catatacacc atccttacct gatgatagat tgtcagtctc
     5221 ttccaatgat actcaggaat ctggaaattc ttcaggacct tcacctggtg ctaagttttc
     5281 ccacatttta caaaaggatg cctttctagt attcaggtca ttgtgtaaac tgtcaatgaa
     5341 accactgtca gatggaccac cagatccaaa gtctcatgaa ctacgatcca agattctttc
     5401 attgcagtta cttctatcca ttctgcagaa tgcaggacct attttcagga caaatgagat
     5461 gtttattaat gctattaagc agtatctttg tgttgcactc tcaaaaaatg gagtctcatc
     5521 tgttccagag gtttttgagc tttctctttc tatatttctt actttgttgt caaatttcaa
     5581 gacacatctg aagatgcaaa ttgaggtgtt ctttaaagaa attttcttat acattttgga
     5641 aacttctacc agctcatttg atcacaaatg gatggttatt cagacactga cgaggatttg
     5701 tgcagatgct cagagtgtag tggatattta tgtaaactat gactgtgact taaatgcagc
     5761 caatatattt gaaagactag taaatgatct atcaaaaatt gctcaaggaa ggggcagtca
     5821 agaacttggt atgagtaatg ttcaggaatt gagcctgagg aaaaaaggtt tagaatgctt
     5881 agtgtcgatt ttgaagtgta tggttgaatg gagtaaggat cagtatgtga atcccaactc
     5941 ccagacaact cttggtcagg aaaaaccctc agagcaagag atgagtgaaa tcaaacaccc
     6001 tgagacaata aacagatacg gaagtttaaa ttccctggag tcaacatcat catcaggaat
     6061 aggcagctac agtacacaga tgtctggcac tgataatcca gaacaatttg aggtcctaaa
     6121 gcaacaaagg taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg
     6181 actttgtcat gcagctccac cgattttgag aacgacagcg acttccgtcc cagccgtgcc
     6241 aggtgctgcc tcagattcag gttatgccgc tcaattcgct gcgtatatcg cttgctgatt
     6301 acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt catccatatc
     6361 accacgtcaa agggtgacag caggctcata agacgcccca gcgtcgccat agtgcgttca
     6421 ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa cagccagcgc
     6481 tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca gacgatgacg
     6541 tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta
     6601 aaatcgtgtt gaggccaacg cccataatgc gggctgttgc ccggcatcca acgccattca
     6661 tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca
     6721 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc
     6781 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
     6841 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcaccagca
     6901 acaaaaagaa ataatagaac aagggataga tttatttaat aagaaaccaa agagaggaat
     6961 acagtacctc caagaacaag ggatgcttgg caccacacct gaagatattg cccaattctt
     7021 acatcaagag gaaagattag actctactca agtgggtgag ttcctgggag ataatgataa
     7081 atttaacaaa gaagtcatgt atgcatatgt ggaccaacat gacttttcag gaaaagactt
     7141 cgtttcagcc cttcgtatgt ttctagaagg atttcgtctt ccaggggaag ctcagaaaat
     7201 cgatcgatta atggaaaaat ttgctgcaag atacctagaa tgcaaccaag gacaaactct
     7261 ctttgctagt gcggatacag cttatgtttt ggcttattca attatcatgt tgaccacaga
     7321 ccttcacagt ccacaggtga aaaataaaat gacaaaggaa caatacatta agatgaatag
     7381 aggtatcaat gacagtaaag accttcctga agagtatcta tcagccatct ataatgaaat
     7441 agctgggaaa aagatatcaa tgaaagaaac aaaagaacta acaatcccta caaaatcaag
     7501 taaacagaat gtagccagtg aaaaacaaag aagacttctg tataacttag aaatggagca
     7561 gatggccaag acagctaaag cactcatgga ggccgtgagc catgttcagg caccctttac
     7621 aagtgcaaca catttggagc atgtgaggcc catgtttaag ttggcttgga cgccttttct
     7681 ggctgcattc agtgtgggtc tacaagattg tgatgatact gaagtagcct ctctttgcct
     7741 ggaaggtata agatgtgcaa tcagaattgc atgcattttc agcattcagc tggagagaga
     7801 tgcatatgtc caggcactag caagatttac cttactcaca gtgagttccg gtattactga
     7861 aatgaaacag aagaacattg acacaataaa aacacttatc acagtggctc atacagatgg
     7921 aaattattta ggaaattcat ggcatgagat tctgaagtgc atcagtcagt tggagctggc
     7981 acagcttata ggaactggag tgaaacctcg atacatttct ggaacagtgc gaggcagaga
     8041 aggatctctt actggaacaa aagatcaggc tcctgatgaa tttgtgggtt tagggctagt
     8101 tggaggaaat gtggactgga aacagatagc aagtattcag gaatccattg gagaaaccag
     8161 ttctcagagt gttgttgttg ctgtagatag aatattcaca ggatctacaa ggctagatgg
     8221 aaatgccatt gtggattttg tccgttggct ctgtgctgtg tctatggatg aattactttc
     8281 cacgacacac ccaagaatgt ttagtctaca aaaaatagta gaaatatcat attacaacat
     8341 gggaagaata agattacagt ggtctcgaat ttgggaagtt attggagatc attttaataa
     8401 ggttgggtgt aatcctaatg aagatgtagc tatttttgca gtagactcct tgaggcagtt
     8461 gtcaatgaag ttcttagaga aaggggagct tgctaacttc agattccaga aggatttctt
     8521 aagacctttt gaacatataa tgaaacggaa caggtctcca acaattcgag atatggttgt
     8581 acggtgtata gcacagatgg ttaattctca agctgctaac attcgatctg gatggaagaa
     8641 cattttctct gtatttcatc tagctgcatc tgatcaagat gaaagcatag tggaacttgc
     8701 attccaaaca accgggcaca ttgtcaccct tgtatttgaa aaacactttc cagcgaccat
     8761 tgattctttc caggatgcag tgaagtgttt gtctgaattt gcgtgcaatg cagctttccc
     8821 agacacaagt atggaagcaa ttcgacttat tcgccattgt gcaaaatatg tgtctgatag
     8881 acctcaggct ttcaaggaat acacaagcga tgatatgaac gtagcacctg aagacagggt
     8941 gtgggtgaga ggatggttcc caattctctt tgagttatcc tgtatcatca atagatgcaa
     9001 attagatgta agaaccaggg gtttaacagt aatgtttgaa ataatgaaaa catatggcca
     9061 cacttatgag aaacactggt ggcaggattt atttagaatt gttttcagaa tctttgacaa
     9121 tatgaaattg ccagaacaac agacagagaa agctgaatgg atgacaacaa cttgcaatca
     9181 tgcactttat gcaatctgtg atgtattcac tcagtattta gaagtactca gtgatgtact
     9241 tttggatgac atttttgctc agctctactg gtgtgtgcag caagacaatg agcagttagc
     9301 gcgatctggt acaaactgtt tagagaatgt tgttattctg aatggtgaaa aatttaccct
     9361 agaaatctgg gataaaactt gcaactgcac actggatatc ttcaaaacca caatcccaca
     9421 tgcgctgttg acctggcgac ccaattctgg agaaactgcc cccccacctc catctcctgt
     9481 aagtgaaaag ccattggata caatatcaca gaagtctgta gatattcatg attctattca
     9541 accaaggtct gtggataaca gaccacaagc accactggtt tctgcgtctg ctgttaatga
     9601 agaagtcagc aaaattaaat ctacagcaaa atttccagaa caaaaattgt ttgctgccct
     9661 gttgattaaa tgtgttgtgc agctggaact catccagact atcgacaaca ttgtcttctt
     9721 cccagccaca agtaagaaag aagatgcaga aaacttagct gcagcacaga gagatgcggt
     9781 ggactttgat gttcgcgttg atactcaaga ccaaggaatg taccgctttt taacatcaca
     9841 acaacttttt aagctactgg actgcttatt agagtcacat agatttgcaa aagcgtttaa
     9901 ttccaacaac gaacagagga ctgccctgtg gaaagcagga ttcaaaggca aatccaagcc
     9961 caaccttctg aagcaggaga ccagcagcct ggcctgtggg ctgcgcattc tcttccggat
    10021 gtacatggat gagagccgcg ttagtgcctg ggaggaggtc cagcagaggc ttttgaatgt
    10081 ccgcagtgaa gcactaagtt acttcctcac tctaacatca gaaagtcatc gagaagcctg
    10141 gactaactta ctgcttttgt ttctaactaa agttctaaag ataagtgata ataggtttaa
    10201 agctcatgca tcattctact accctctctt atgtgaaatt atgcaatttg acttgattcc
    10261 tgaacttcgt gctgttctta gaagattttt tctgcgaatc ggagtagttt ttcagatatc
    10321 acaaccacct gaacaggaac ttggaataaa caagcaatga tgggaactta atatttttgt
    10381 tggcatttac atccctctgc atcgaattcc tgcagcccgg gggatccact agttctagag
    10441 cggccgctcg agatatctag acccagcttt cttgtacaaa gtggttgatc tagagggccc
    10501 gcggttcgaa ctcgaggtac cgaattcact cctcaggtgc aggctgccta tcagaaggtg
    10561 gtggctggtg tggccaatgc cctggctcac aaataccact gagatcgatc tttttccctc
    10621 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
    10681 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
    10741 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
    10801 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
    10861 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
    10921 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
    10981 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
    11041 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
    11101 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    11161 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    11221 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
    11281 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    11341 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    11401 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11461 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    11521 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11581 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    11641 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    11701 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    11761 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    11821 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11881 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11941 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    12001 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    12061 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    12121 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    12181 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    12241 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    12301 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    12361 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    12421 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    12481 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    12541 aaaaatgaag ttttaaatca atctaaagta tata
//
