LOCUS       sequence-454237-        9337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9337)
  TITLE     Mammalian expression plasmids and entry plasmids for Gateway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR/HA-hBIG2
FEATURES             Location/Qualifiers
     source          1..9337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      82..168
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      260..287
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     295..318
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    337..436
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             475..501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(7545..7644)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7756..7775)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7767..8576
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7834..7853)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8666..9254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9155..9174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgctagcat ggatctcggg gacgtctaac tactaagcga
       61 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt
      121 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
      181 gatttgaacg ttgtgaagca acggcccgga gggtggcggg caggacgccc gccataaact
      241 gccaggcatc aaactaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
      301 actcttcctg ttagttagtt acttaagctc gggccccaaa taatgatttt attttgactg
      361 atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat gccaactttg
      421 tacaaaaaag caggctcttt aaaggaacca attcagtcga cggtacctac catgtacccg
      481 tacgacgtgc ccgactacgc gggatccacc atgcaggaga gccagaccaa gagcatgttc
      541 gtgtcccggg ccctggagaa gatcctagcc gacaaggagg tgaagcggcc ccagcactcc
      601 cagctgcgca gggcctgcca ggtggcgctc gatgaaatta aagcagaaat agaaaagcag
      661 aggcttggca ctgctgcacc accaaaggca aacttcattg aagctgacaa gtattttctt
      721 ccattcgagc tagcttgcca gtccaagtcc ccaagggtag tcagcacatc ccttgactgc
      781 ttgcagaaac tcatcgcata cgggcacatc actggcaacg cccctgacag tggagcccct
      841 gggaagcggc tgatcgacag aattgttgaa accatttgca gttgttttca gggccctcag
      901 actgatgaag gggttcagtt acaaataatt aaggctcttc tgactgcagt gacttcccca
      961 cacattgaaa ttcatgaggg tactatcctg cagacagtga gaacatgtta caatatctat
     1021 ttggccagca aaaatctcat caatcaaacc actgccaagg ctacccttac tcagatgctg
     1081 aacgtcattt tcacccgcat ggaaaaccaa gtgttgcagg aggccagaga actggaaaaa
     1141 ccaatccagt caaaacccca gtcccctgtg atccaagctg cagcagtatc cccaaagttc
     1201 gttcgtttga agcacagtca ggcacaaagc aaaccaacaa ctcccgaaaa aacagattta
     1261 accaacggtg aacatgccag gagtgattct ggaaaagtaa gcacagaaaa tggagacgca
     1321 cccagagaaa gaggctcatc actgtcaggg actgatgacg gagcccagga ggtggtgaag
     1381 gacatcttgg aagatgtagt cacatctgcc attaaagcag cggaaaagca tggtctgaca
     1441 gaacctgaga gagttctagg tgaactggag tgccaggaat gtgctattcc cccaggagtt
     1501 gatgaaaact cacagaccaa cgggatagcc gatgacaggc agtccttgtc gtcagcagat
     1561 aatctggaat cggatgcaca aggacatcaa gtggctgcca ggttctccca cgttctgcag
     1621 aaggatgcct tccttgtgtt ccgctccctg tgcaagctgt ccatgaaacc ccttggtgaa
     1681 ggccctccag acccaaaatc ccatgagctg cgttccaagg tggtttccct gcagctgctc
     1741 ctctctgtgt tgcaaaatgc tggccccgta ttcaggactc acgagatgtt catcaatgca
     1801 atcaagcaat atctctgtgt ggccttgtcc aaaaacggcg tctcttcagt gcctgatgtc
     1861 tttgagctct ctcttgccat ttttcttact cttctttcaa actttaaaat gcacttgaaa
     1921 atgcagatag aggtcttttt caaagagatt ttcctgaaca ttttagaaac atcaacaagt
     1981 tcttttgagc acaggtggat ggtcattcag actctgacga ggatctgtgc agatgcccag
     2041 tgtgttgtgg atatttatgt caactacgac tgtgatttaa atgctgctaa catttttgag
     2101 cgccttgtaa atgatttatc caaaattgct cagggaagaa gtggacatga gctgggaatg
     2161 acacctctgc aggagctcag cctgaggaag aaaggcctgg agtgcctcgt gtccattctc
     2221 aagtgcatgg tggagtggag caaagacctg tatgtgaatc ccaaccacca gaccagcctc
     2281 ggtcaggaga ggctcacgga tcaggaaata ggggatggga aaggccttga catggcaaga
     2341 cggtgtagtg tgacgtccat ggagtccaca gtgtcctcgg ggacccagac aactgttcag
     2401 gatgaccctg agcaatttga ggtcatcaag caacaaaaag aaatcattga acacggcatc
     2461 gagctgttca acaagaaacc caagaggggg atccagtttc tccaggagca gggcatgctg
     2521 ggaacgtcag ttgaagacat agcccaattc ctgcaccagg aggagcgcct ggattccacc
     2581 caagtaggcg attttctggg agatagcgca aggttcaaca aggaggtgat gtatgcctac
     2641 gtggaccaac ttgacttctg tgaaaaagaa tttgtctcag ccctgcggac attcctagaa
     2701 ggtttccgcc tacctggaga agcccaaaag attgaccgat taatggagaa gtttgccgca
     2761 agatacatag aatgcaacca agggcaaact ctgtttgcta gtgctgacac tgcttatgtc
     2821 ctagcgtatt caattattat gctgactaca gacttgcaca gtcctcaggt aaaaaataaa
     2881 atgacgaaag agcagtatat taaaatgaat cggggtatca atgatagtaa agatctgcca
     2941 gaagagtatc tctcaagcat ctatgaagag atagaaggca agaaaattgc aatgaaagaa
     3001 acaaaagagc taaggaaggt gcgaataagc ggggaaattc ttctcggctg actcagtcat
     3061 ttcatttctt catgtttgag ccgatttttt ctcccgtaaa tgccttgaat cagcctattt
     3121 agaccgtttc ttcgccattt aaggcgttat ccccagtttt tagtgagatc tctcccactg
     3181 acgtatcatt tggtccgccc gaaacaggtt ggccagcgtg aataacatcg ccagttggtt
     3241 atcgtttttc agcaacccct tgtatctggc tttcacgaag ccgaactgtc gcttgatgat
     3301 gcgaaatggg tgctccaccc tggcccggat gctggctttc atgtattcga tgttgatggc
     3361 cgttttgttc ttgcgtggat gctgtttcaa ggttcttacc ttgccggggc gctcggcgat
     3421 cagccagtcc acatccacct cggccagctc ctcgcgctgt ggcgcccctt ggtagccggc
     3481 atcggctgag acaaattgct cctctccatg cagcagatta cccagctgat tgaggtcatg
     3541 ctcgttggcc gcggtggtga ccaggctgtg ggtcaggcca ctcttggcat cgacaccaat
     3601 gtgggccttc atgccaaagt gccactgatt gcctttcttg gtctgatgca tctccggatc
     3661 gcgttgctgc tctttgttct tggtcgagct gggtgcctca atgatggtgg catcgaccaa
     3721 ggtgccttga gtcatcatga cgcctgcttc ggccagccag cgattgatgg tcttgaacaa
     3781 ttggcgggcc agttgatgct gctccagcag gtggcggaaa ttcatgatgg tggtgcggtc
     3841 cggcaaggcg ctatccaggg ataaccgggc aaacagacgc atggaggcga tttcgtacag
     3901 agcatcttcc atcgcgccat cgctcaggtt gtaccaatgc tgcatgcagt gaatgcgtag
     3961 catggtttcc agcggataag gtcgccggcc attaccagcc ttggggtaaa acggctcgat
     4021 gacttccacc atgttttgcc atggcagaat ctgctccatg cgggacaaga aaatctcttt
     4081 tctggtctga cggcgcttac tgctgaattc actgtcggcg aaggtaagtt gatgactcat
     4141 gatgaaccct gttctatggc tccagatgac aaacatgatc tcatatcagg gatttgttcg
     4201 caccttccct aacgattgca accaaatcta ctaagcagaa tgtagctagt gaaaagcagc
     4261 ggcggctgct gtacaactta gagatggagc aaatggctaa aacagccaaa gctctgatgg
     4321 aggctgtgag ccatgccaaa gccccgttta ccagtgccac tcacctggac catgtccggc
     4381 caatgttcaa actggtgtgg acgccactat tggcagccta cagcatcgga ctccagaact
     4441 gtgatgacac tgaagtggcc tccttgtgtt tggaaggcat ccgatgtgca atccgaatcg
     4501 cctgcatctt tggaatgcag ctggaacgag atgcctatgt tcaggctctt gctcgcttct
     4561 ccctactcac agccagctcc agcatcacag aaatgaagca gaaaaacatc gacaccatta
     4621 agacgcttat cacagtggct cacaccgatg gcaactacct tgggaattcc tggcatgaga
     4681 tcttgaaatg catcagccag ctggagctcg ctcagctgat aggaaccggt gtgaagacgc
     4741 gctacctgtc tggatctggg cgtgaaagag aagggagcct gaagggccac acattggcag
     4801 gagaagagtt catgggcctt ggcctcggta atttggtgag tggcggagtg gataaaagac
     4861 agatggccag cttccaagaa tcggttggtg agaccagctc gcagagtgtg gttgtagctg
     4921 tggacaggat ttttactggg tctaccagac tggatggaaa tgcaatagtt gactttgtcc
     4981 gctggctgtg tgctgtgtcc atggatgaac tggcttcccc ccaccatcct cgcatgttca
     5041 gcttgcagaa gattgtggag atatcatact acaacatgaa tcggatccga ctacagtggt
     5101 ctcgaatatg gcatgtgatt ggagatcact tcaataaggt tggctgcaac cctaatgaag
     5161 atgtggctat ctttgctgtt gactcattaa ggcaactctc catgaagttt cttgagaagg
     5221 gtgaattagc caacttccgt ttccagaaag attttctgag gccctttgag catattatga
     5281 agaaaaacag gtctcccacc atccgggaca tggcgatccg ctgcattgcc cagatggtga
     5341 actcccaggc ggccaacatc cgctcaggct ggaagaacat ctttgccgtg ttccaccagg
     5401 cagcctctga tcatgatggg aacattgtgg agctggcctt ccagaccact tgccacattg
     5461 tcacaactat tttccagcac cattttcctg cagccatcga ttcctttcag gatgctgtga
     5521 agtgcttatc agagttcgcc tgcaacgccg ctttccctga cacgagcatg gaagcgattc
     5581 ggctcatccg cttctgtggc aaatacgtct ctgagaggcc tcgggtgcta caagaataca
     5641 caagtgatga catgaatgta gctcctggtg acagagtctg ggtccgaggc tggttcccca
     5701 tcttattcga actctcctgc atcattaata gatgcaagtt agatgtacga acaaggggac
     5761 tcacagtcat gtttgagatc atgaagagct atggccacac ctttgaaaag cactggtggc
     5821 aggacctgtt cagaatcgtg tttcggattt ttgacaatat gaaactccct gagcaactgt
     5881 cagagaaatc tgagtggatg acaacaacct gcaatcacgc actttatgct atttgtgatg
     5941 tttttaccca gttttatgaa gctttgaatg aagttcttct ttctgatgta tttgcacaat
     6001 tgcagtggtg tgtcaaacaa gataatgaac agttggcgcg atcaggtaca aattgcttag
     6061 aaaacttagt aatatccaat ggagagaaat tcagtcctga agtctgggat gaaacctgca
     6121 actgtatgtt ggatattttc aaaacaacca tcccacatgt tttgctgaca tggagacctg
     6181 taggaatgga ggaagattca tcagaaaagc atttggatgt ggatctggac cgccagtctt
     6241 taagcagcat agataaaaat ccctctgaga ggggacagag ccagctctct aacccaacag
     6301 atgacagctg gaagggtaga ccatacgcaa atcagaaact gtttgccagc ctcctcatca
     6361 agtgtgtggt ccagttggaa ttgatacaga ccattgacaa cattgtgttc taccctgcga
     6421 cgagcaaaaa ggaggatgca gagcacatgg ttgccgccca gcaagacacg ctggatgcag
     6481 atatccacat agagacggag gatcagggca tgtataagta catgtcttcc cagcacctct
     6541 tcaagctgtt ggactgtttg caggaatccc attcattctc aaaggccttc aactccaatt
     6601 acgagcagcg gactgtcctg tggcgagcag gttttaaggg caagtctaaa cccaatcttc
     6661 taaaacaaga aaccagcagc ctggcctgtt gtttgaggat cctgtttcga atgtatgttg
     6721 atgagaaccg cagggattcc tgggaagaaa tacagcagag acttttaact gtttgcagtg
     6781 aagctcttgc ctatttcatc actgtgaatt ctgagagcca tcgggaggcc tggacaagtc
     6841 tcttgttgtt acttctaact aaaaccctta agataaatga tgaaaagttc aaagcacatg
     6901 cttcaatgta ctacccctac ttgtgtgaaa ttatgcagtt tgacctgatc cctgagctcc
     6961 gagcagttct gcggaagttc ttcctacgga taggtgttgt gtataagata tggataccag
     7021 aagagccatc acaggtacca gcagcactgt caccagtgtg gtagccctgg ctgcccaggc
     7081 cagtgctgca gctctgcaga atgttcagca tgccatttct gactggcaca tctcgtgaag
     7141 tttcatagaa acaaggagtt ggcatcttgg atctcagaat ggcctggaaa cggatggcct
     7201 ctacgctgtt ccatcacagt ctccaactaa ggcttatggt atttcattaa actgttgcat
     7261 acccagttag cacagtaggt ggggagtctg cttcatttct atcattccat ttttctgatt
     7321 aaactgtcaa atctgtcatt gcatatgcca tcgttttcta gcaaaatccc atgattggct
     7381 ataaacgttt tgtaagaagt cactctcctt gaaaatactg aacatagctg tataggtttg
     7441 tgattattag agaatatgtt aataaaactt cttgtaacgg ctaactgcca cctaaaataa
     7501 aaaaaaaaaa aaaaaacgtt tgcggccgca ctcgagatat ctagacccag ctttcttgta
     7561 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     7621 cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc
     7681 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     7741 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     7801 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     7861 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     7921 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     7981 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     8041 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     8101 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     8161 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     8221 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     8281 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     8341 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     8401 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     8461 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     8521 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     8581 aattggttaa ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc
     8641 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8701 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8761 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8821 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8881 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8941 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9001 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9061 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9241 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9301 cttttgctca catgttcttt cctgcgttat cccctga
//
