LOCUS       sequence-45425-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE0c-RFP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             143..154
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(163..197)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        236..338
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(405..424)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtttagcggt ctgggtacct tcgtacggac gaccttcacc ttcaccttcg atttcgaact
       61 cgtgaccgtt aacggaacct tccatacgaa ctttgaaacg catgaactct ttgataacgt
      121 cttcggagga agccatctag tatttctcct ctttcactag tagctagcac agtacctagg
      181 actgagctag ctgtcaaaga tctcatgaat tcgatatctg gcgaaaatga gacgttgatc
      241 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      301 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      361 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      421 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      481 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      541 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
      601 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      661 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      721 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
      781 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
      841 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
//
