LOCUS       sequence-45427-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE0c-RFP
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      160..231
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      247..274
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      328..399
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      415..442
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(680..699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
       61 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      121 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      181 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      241 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaagc ggccgggatc
      301 ctaactcgag taaggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      361 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      421 ccttcgggtg ggcctttctg cgtttatacc tagggcgttc ggctgcggcg agcggtatca
      481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgc
//
