LOCUS       sequence-454319-        7608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TAM Receptor Ligand human PROS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Geng K, Kumar S, Kimani SG, Kholodovych V, Kasikara C, Mizuno K, 
            Sandiford O, Rameshwar P, Kotenko SV, Birge RB
  TITLE     Requirement of Gamma-Carboxyglutamic Acid Modification and 
            Phosphatidylserine Binding for the Activation of Tyro3, Axl, and 
            Mertk Receptors by Growth Arrest-Specific 6.
  JOURNAL   Front Immunol. 2017 Nov 10;8:1521. doi: 10.3389/fimmu.2017.01521. 
            eCollection 2017.
  PUBMED    29176978
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2017-11-10"; volume: "8:1521. doi"; pages: " 
            10.3389/fimmu.2017.01521. eCollection 2017"
COMMENT     Sequence Label: pSecTaq2-hProS-Myc-6xHis
FEATURES             Location/Qualifiers
     source          1..7608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      576..1164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1065..1084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1318..1335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(1488..1493,1494..1511,1512..1518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1526..1542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1531..1553
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1550..1566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1550..1566
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1615..1736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1626..1645)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1680..1699
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1866..2891)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1866..2891)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(2940..3269)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2954..3089
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3008..3027)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3254..3274
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3283..3711)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3393..3414)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3605..3624
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3757..3981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3970..3987
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4010..4027)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4043..4072)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     sig_peptide     complement(6053..6112)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     regulatory      6109..6118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6135..6154)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6136..6154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6199..6402)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6228..6248)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6403..6782
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6954..6973
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7048..7152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7153..7221,7222..7608,1..405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7371..7390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
       61 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
      421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      721 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1321 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1381 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     1441 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cccaggcttt acactttatg
     1501 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1561 tatgaccatg attacgccaa gctctagcta gaggtcgacg gtatacagac atgataagat
     1621 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     1681 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgggg
     1741 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
     1801 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtagc
     1861 acgtgctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     1921 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     1981 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     2041 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     2101 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     2161 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     2221 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     2281 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     2341 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     2401 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     2461 tattgaccga ttccttgcgg tccaaatggg ccgaacccgc tcgtctggct aagatcggcc
     2521 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     2581 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     2641 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     2701 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     2761 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     2821 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     2881 ggctttttca tcacgtgctg atcagatccg aaaatggata tacaagctcc cgggagcttt
     2941 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca
     3001 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     3061 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     3121 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     3181 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     3241 ggggactttc cacaccctaa ctgacacaca ttccacagaa ttaattcgcg ttaaattttt
     3301 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3361 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3421 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3481 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3541 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3601 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3661 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatacc
     3721 ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
     3781 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     3841 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     3901 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     3961 gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaactcaa tgatgatgat
     4021 gatgatggtc gacggcgcta ttcagatcct cttctgagat gagtttttgt tcgggccctc
     4081 ctcgagcggc cgcagaattt ttcgtttttt tccatacact tggacaacta tgagctcgta
     4141 tatcgttatg cttgctgatg gcctcgtcca aatccagctg aactccgttg atgttaacct
     4201 ccatgcatcc attgtaaaaa gcattaactg gcgtggcgga aaatggtaca tcagggaggc
     4261 cgccaaggta ggtggctact ttggctttca tagctttgtc cagaactgcc agttggcgtt
     4321 gcaggtcctc atgtgatatg gtctcaattt tgagaggggt gctcaattca agattgtttc
     4381 ggttgactct aaactccaag tgagattgct gatcggaaca caagctcaat gcctgaatgc
     4441 gataaatgac agtattttcc acagacaaca gtatatcctg agacttctca ctagtggagt
     4501 cgacaaggga cactgcgaag gggacagtgt tgttaccaga aaccagtgcg agcatgaccc
     4561 ccgttccggt actaggccga atattaagag ttacattcac gtgccaacct tccgcactgc
     4621 tgacgttatt atagtcgatg tgaaactgcg ctataccaga tccgggataa taggagccct
     4681 tctcaactgt aaccaggcag tgtttatttt gtttctcttg tatgatctcc tttatgccag
     4741 acgcaccttg cttcatcaga ttccaacttc gaatgcagcc atcaagccgg gggtttatag
     4801 gtttgataag ttcggattct acctttcggg gaaagcctgc gaagtagacc tttgtttcca
     4861 gcaaaccgtt ctcaggttta aaaagggggc cgggtttgtt tatgtccatg actgcttctt
     4921 tggcaatctt tatgcttatg gagtgctcaa gttcctctac actcaccatg ttccagaggc
     4981 cattatttat gacgtccccg cccgtggtta tcttagacgt atgttcattc ttgagctgta
     5041 cttctatttt acctcctctg agggcaatga gaagccaggc agaatgatcg attgactctg
     5101 catacagtat caccccttca gagtcatacg tgcggaaatc aaattcagca ctaaatctag
     5161 agatttccgg aagtcgaaac ttcaggtaaa gaactacccc cgcgaattgc tctgccaagt
     5221 acaacaattc atacttagtg tccaaattca acggcaaaca tacagacaca acttcacaag
     5281 acttctggtc ttgagcaagt ttaaatccct ttttcccatc acagtaacag gtatacccac
     5341 caggatagtt cacacaaagt tgagcacaca tattttcgga gcactcgtca atatcctcac
     5401 aagatttaga cttcaaatta tagcggtagc cctctggaca ctcacactcg aaatcccctg
     5461 gtatgttctt acaaactgct gttccgcata tactgggctt caggctgcac tcatcaacgt
     5521 ctttacagtc ctttttgtta gacaacatga caaatccatt cttgcaagaa caatgatagg
     5581 aaccaggtgt attgtcacag atctgactgc aaccgccgtt aatgttactt gggtccttgc
     5641 attcgtttat atcgaactca cacttctcac cttgccaccc cggtttacaa gtgcatgtaa
     5701 atgaagcttt tccgtcctta caagacatgt atccgtcttc gttgcacggg aggggtgaac
     5761 attgatctgg tatcgcattg acacagcttc ggagatcagg atatgcgttg gtgctttgtc
     5821 tggcggccgt aaacaatccc gtctgaaagc ttcgaagaca tacaaggtat ttcgggtaaa
     5881 agtaatcagt ttcaggatca ttttcaaata cttccctcgc ttcctccttg ttgcacaact
     5941 cttctataca ttcgcgttca aggttcccct gcttggtctc ttcaagcaat gagttggctc
     6001 tgcgtttgcg aacaagaact tgactcgctt gttgtttact aagaaagttg tcaccagtgg
     6061 aacctggaac ccagagcagc agtacccata gcaggagtgt gtctgtctcc atggtggcta
     6121 gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt aagcagtggg
     6181 ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     6241 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     6301 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     6361 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     6421 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     6481 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     6541 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     6601 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     6661 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     6721 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     6781 tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
     6841 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
     6901 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
     6961 agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg
     7021 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaac
//
