LOCUS       sequence-454327-        8303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a chimeric contruct having N-terminal human MerTK and 
            C-terminal human Interferon Gamma Receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Tsou WI, Nguyen KQ, Calarese DA, Garforth SJ, Antes AL, Smirnov SV, 
            Almo SC, Birge RB, Kotenko SV
  TITLE     Receptor tyrosine kinases, TYRO3, AXL, and MER, demonstrate distinct
            patterns and complex regulation of ligand-induced activation.
  JOURNAL   J Biol Chem. 2014 Sep 12;289(37):25750-63. doi: 
            10.1074/jbc.M114.569020. Epub 2014 Jul 29.
  PUBMED    25074926
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.569020"; journalName: "J Biol Chem"; date: 
            "2014-09-12- 12"; volume: "289"; issue: "37"; pages: "25750-63"
COMMENT     Sequence Label: pEF2-FL-hMer_gR1
FEATURES             Location/Qualifiers
     source          1..8303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(106..125)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     316..337
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(456..476)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        461..790
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      641..776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     703..722
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             857..1651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(911..930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1521..1540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1825..1946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1862..1881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1916..1935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1995..2011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1995..2011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2008..2030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2019..2035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2043..2049,2050..2067,2068..2073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2088..2109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2226..2243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2397..2985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2477..2496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3156..3947,3948..4016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3779..3798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4017..4121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4196..4215)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4503..5681
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4734..5672
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5629..5649
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5779..5802
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(8008..8026)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8009..8026)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8046..8063)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8052..8276
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
       61 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      241 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      301 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      421 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
      481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      541 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      601 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      721 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      781 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
      841 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
      901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
      961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     1021 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     1081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     1141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     1201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     1261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     1321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     1381 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     1441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     1501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     1561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     1621 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     1681 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     1741 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     1801 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     1861 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1921 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     1981 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2041 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2101 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2161 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2221 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2281 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2341 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2401 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2461 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2521 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2581 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2641 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2701 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2761 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2821 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2881 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2941 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3001 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3061 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3121 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3181 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3241 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3301 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3361 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3421 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3481 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3541 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3601 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3661 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3721 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3781 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3841 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3901 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3961 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4021 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4081 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4141 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     4201 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     4261 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     4321 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     4381 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
     4441 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
     4501 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4561 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4621 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4681 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4741 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4801 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     4861 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     4921 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     4981 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     5041 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     5101 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     5161 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     5221 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     5281 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     5341 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     5401 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     5461 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     5521 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     5581 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     5641 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgga
     5701 gctcggatcg gggccatgcg accgacgctg ctgtggtcgc tgctgctgct gctcggagtc
     5761 ttcgccgccg ccgccgccga ctacaaggac gacgatgaca aggcggatcc cgctatcact
     5821 gaggcaaggg aagaagccaa gccttacccg ctattcccgg gaccttttcc agggagcctg
     5881 caaactgacc acacaccgct gttatccctt cctcacgcca gtgggtacca gcctgccttg
     5941 atgttttcac caacccagcc tggaagacca catacaggaa acgtagccat tccccaggtg
     6001 acctctgtcg aatcaaagcc cctaccgcct cttgccttca aacacacagt tggacacata
     6061 atactttctg aacataaagg tgtcaaattt aattgctcaa tcagtgtacc taatatatac
     6121 caggacacca caatttcttg gtggaaagat gggaaggaat tgcttggggc acatcatgca
     6181 attacacagt tttatccaga tgatgaagtt acagcaataa tcgcttcctt cagcataacc
     6241 agtgtgcagc gttcagacaa tgggtcgtat atctgtaaga tgaaaataaa caatgaagag
     6301 atcgtgtctg atcccatcta catcgaagta caaggacttc ctcactttac taagcagcct
     6361 gagagcatga atgtcaccag aaacacagcc ttcaacctca cctgtcaggc tgtgggcccg
     6421 cctgagcccg tcaacatttt ctgggttcaa aacagtagcc gtgttaacga acagcctgaa
     6481 aaatccccct ccgtgctaac tgttccaggc ctgacggaga tggcggtctt cagttgtgag
     6541 gcccacaatg acaaagggct gaccgtgtcc aagggagtgc agatcaacat caaagcaatt
     6601 ccctccccac caactgaagt cagcatccgt aacagcactg cacacagcat tctgatctcc
     6661 tgggttcctg gttttgatgg atactccccg ttcaggaatt gcagcattca ggtcaaggaa
     6721 gctgatccgc tgagtaatgg ctcagtcatg atttttaaca cctctgcctt accacatctg
     6781 taccaaatca agcagctgca agccctggct aattacagca ttggtgtttc ctgcatgaat
     6841 gaaataggct ggtctgcagt gagcccttgg attctagcca gcacgactga aggagcccca
     6901 tcagtagcac ctttaaatgt cactgtgttt ctgaatgaat ctagtgataa tgtggacatc
     6961 agatggatga agcctccgac taagcagcag gatggagaac tggtgggcta ccggatatcc
     7021 cacgtgtggc agagtgcagg gatttccaaa gagctcttgg aggaagttgg ccagaatggc
     7081 agccgagctc ggatctctgt tcaagtccac aatgctacgt gcacagtgag gattgcagcc
     7141 gtcaccaaag ggggagttgg gcccttcagt gatccagtga aaatatttat ccctgcacac
     7201 ggttgggtag attatgcccc ctcttcaact ccggcgcctg gcaacgcaga tccttggcta
     7261 gcgccagttg ttgctgcttt actactcttt ctagtgctta gcctggtatt catctgtttt
     7321 tatattaaga aaattaatcc attgaaggaa aaaagcataa tattacccaa gtccttgatc
     7381 tctgtggtaa gaagtgctac tttagagaca aaacctgaat caaaatatgt atcactcatc
     7441 acgtcatacc agccattttc cttagaaaag gaggtggtct gtgaagagcc gttgtctcca
     7501 gcaacagttc caggcatgca taccgaagac aatccaggaa aagtggaaca tacagaagaa
     7561 ctttctagta taacagaagt ggtgactact gaagaaaata ttcctgacgt ggtcccgggc
     7621 agccatctga ctccaataga gagagagagt tcttcacctt taagtagtaa ccagtctgaa
     7681 cctggcagca tcgctttgaa ctcgtatcac tccagaaatt gttctgagag tgatcactcc
     7741 agaaatggtt ttgatactga ttccagctgt ctggaatcac atagctcctt atctgactca
     7801 gaatttcccc caaataataa aggtgaaata aaaacagaag gacaagagct cataaccgta
     7861 ataaaagccc ccacctcctt tggttatgat aaaccacatg tgctagtgga tctacttgtg
     7921 gatgatagcg gtaaagagtc cttgattggt tatagaccaa cagaagattc caaagaattt
     7981 tcatgaattc ctactagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     8041 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     8101 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     8161 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     8221 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     8281 tgaggcggaa agaaccagct ggg
//
