LOCUS       sequence-454330-        5421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA donor for transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Yang S, Zhu J, Zhou X, Zhang J, Li Q, Bian F, Zhu J, Yan T, Wang X, 
            Zhang Y, Yang J, Jiang Y, Yang S
  TITLE     RNA-Guided DNA Transposition in Corynebacterium glutamicum and 
            Bacillus subtilis.
  JOURNAL   ACS Synth Biol. 2023 Jul 21;12(7):2198-2202. doi: 
            10.1021/acssynbio.3c00193. Epub 2023 Jun 22.
  PUBMED    37348112
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00193"; journalName: "ACS Synth Biol"; date: 
            "2023-07-21- 21"; volume: "12"; issue: "7"; pages: "2198-2202"
COMMENT     Sequence Label: pBsVchDonor
FEATURES             Location/Qualifiers
     source          1..5421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     574..596
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     588..605
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     589..605
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     615..634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        615..633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3982..3993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttattgaga ggagggatta ttgaataaat aaaagccccc tgacgaaagt cgaagggggt
       61 ttttattttg gtttgatgtt gcgattaata gcaatacaat tgcaataaac aaaatgatct
      121 tccttcaggt tatgaccatc tgtgccagtt cgtaatgtct ggtcaacttt ccgactctga
      181 gaaacttctg gaatcgctag agaatttctg gaatgggatt caggagtgga cagaacgaca
      241 cggatatata gtggatgtgt caaaacgcat accattttga acgatgacct ctaataattg
      301 ttaatcatgt tggttacgta tttattaact tctcctagta ttagtaatta tcatggctgt
      361 catggcgcat taacggaata aagggtgtgc ttaaatcggg ccattttgcg taataagaaa
      421 aaggattaat tatgagcgaa ttgaattaat aataaggtaa tagatttaca ttagaaaatg
      481 aaaggggatt ttatgcgtga gaatgttaca gtctatccct ggcgaaaggg ggatgtgctg
      541 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      601 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgt tgatacaacc
      661 ataaaatgat aattacaccc ataaattgat aattatcaca cccataaatt gatattgcct
      721 cttcatggtc taaacttcag taagtttacg acattttcat gaagaggtca tttccgggga
      781 tccgcatgac atttcagcat aatgaacatt tactcatgtc tattttcgtt cttttctgta
      841 tgaaaatagt tatttcgagt ctctacggaa atagcgagag atgatatacc taaatagaga
      901 taaaatcatc tcaaaaaaat gggtctacta aaatattatt ccatctatta caataaattc
      961 acagaatagt cttttaagta agtctactct gaattttttt aaaaggagag ggtaaagagt
     1021 gagaagcaaa aaattgtgga tcagcttgtt gtttgcgtta acgttaatct ttacgatggc
     1081 gttcagcaac atgtctgcgc aggctgccgg aaaaagcagt acagaaaaga aatacattgt
     1141 cggatttaaa cagacaatga gtgccatgag ttccgccaag aaaaaggatg ttatttctga
     1201 aaaaggcgga aaggttcaaa agcaatttaa gtatgttaac gcggccgcag caacattgga
     1261 tgaaaaagct gtaaaagaat tgaaaaaaga tccgagcgtt gcatatgtgg aagaagatca
     1321 tattgcacat gaatatgcgc aatctgttcc ttatggcatt tctcaaatta aagcgccggc
     1381 tcttcactct caaggctaca caggctctaa cgtaaaagta gctgttatcg acagcggaat
     1441 tgactcttct catcctgact taaacgtcag aggcggagca agcttcgtac cttctgaaac
     1501 aaacccatac caggacggca gttctcacgg tacgcatgta gccggtacga ttgccgctct
     1561 taataactca atcggtgttc tgggcgtagc gccaagcgca tcattatatg cagtaaaagt
     1621 gcttgattca acaggaagcg gccaatatag ctggattatt aacggcattg agtgggccat
     1681 ttccaacaat atggatgtta tcaacatgag ccttggcgga cctactggtt ctacagcgct
     1741 gaaaacagtc gttgacaaag ccgtttccag cggtatcgtc gttgctgccg cagccggaaa
     1801 cgaaggttca tccggaagca caagcacagt cggctaccct gcaaaatatc cttctactat
     1861 tgcagtaggt gcggtaaaca gcagcaacca aagagcttca ttctccagcg caggttctga
     1921 gcttgatgtg atggctcctg gcgtgtccat ccaaagcaca cttcctggag gcacttacgg
     1981 cgcttataac ggaacgtcca tggcgactcc tcacgttgcc ggagcagcag cgttaattct
     2041 ttctaagcac ccgacttgga caaacgcgca agtccgtgat cgtttagaaa gcactgcaac
     2101 atatcttgga aactctttct actatggaaa agggttaatc aacgtacaag cagctgcaca
     2161 ataatagtaa aaagaagcag gttcctccat acctgcttct ttctagagtc cttactgcag
     2221 tagttttgct gaaatactcg attcacaaaa atatcaactt atggttgttt tgtgagatat
     2281 caatatatgg ttgttttgtg gttaagttgc tgattataaa taattattaa atatcacttt
     2341 atggttgcat caacagaatt cgcggccgcc atatttccag ggagcagccg cggccggtgc
     2401 tttgcaggat tgggatcctc tagagtccgc tagggacctc tttagctcct tggaagctgt
     2461 cagtagtata cctaataatt tatctacatt ccctttagta acgtgtaact ttccaaattt
     2521 acaaaagcga ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag
     2581 acaatacttg ctcataagta acggtactta aattgtttac tttggcgtgt ttcattgctt
     2641 gatgaaactg atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa
     2701 agtacgtata tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta
     2761 ttaagacact gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt
     2821 gctgaatcga gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac
     2881 gcttgtagaa tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca
     2941 cttttttaat aatttgtgtg cttaaatggt aaggaatact cccaacaatt ttatacctct
     3001 gtttgttagg gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgagtattca
     3061 gttttaattt ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct
     3121 gtttacttat tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa
     3181 cggtatcggt ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg
     3241 ttaaaaagtt ttgagaatat tttatatttt tgttcatgta atcactcctt cttaattaca
     3301 aatttttagc atctaattta acttcaattc ctattataca aaattttaag atactgcact
     3361 atcaacacac tcttaagttt gcttctaagt cttatttcca taacttcttt tacgtttccg
     3421 ccattctttg ctgtttcgat ttttatgata tggtgcaagt cagcacgaac acgaaccgtc
     3481 ttatctccca ttatatcttt ttttgcactg attggtgtat catttcgttt ttcttttgcg
     3541 cgactctaga ggatcctgat aaatatgaac atgatgagtg atcgttaaat ttatactgca
     3601 atcggatgcg attattgaat aaaagatatg agagatttat ctaatttctt ttttcttgta
     3661 aaaaaagaaa gttcttaaag gttttatagt tttggtcgta gagcacacgg tttaacgact
     3721 taattacgaa gtaaataagt ctagtgtgtt agactttatg aaatctatat acgtttatat
     3781 atatttatta tccgattttt tattaaaacg tctcaaaatc gtttctgaga cgttttagcg
     3841 tttatttcgt ttagttatcg gcataatcgt taaaacaggc gttatcgtag cgtaaaagcc
     3901 cttgagcgta gcgtggcttt gcagcgaaga tgttgtctgt tagcttatga aagccgatga
     3961 ctgaatgaaa taataagcgc agcgcccttc tatttcggtt ggaggaggct caagggagta
     4021 tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat tttgccgagc
     4081 ggtagcgctg gaaaattttt gaaaaaaatt tggaatttgg aaaaaaatgg ggggaaagga
     4141 agcgaatttt gcttccgtac tacgaccccc cattaagtgc cgagtgccaa tttttgtgcc
     4201 aaaaacgctc tatcccaact ggctcaaggg tttaaggggt ttttcaatcg ccaacgaatc
     4261 gccaacgttt tcgccaacgt tttttataaa tctatattta agtagcttta ttgttgtttt
     4321 tatgattaca aagtgataca ctaactttat aaaattattt gattggagtt ttttaaatgg
     4381 tgatttcaga atcgaaaaaa agagttatga tttctctgac aaaagagcaa gataaaaaat
     4441 taacagatat ggcgaaacaa aaaggttttt caaaatctgc ggttgcggcg ttagctatag
     4501 aagaatatgc aagaaaggaa tcagaacaaa aaaaataagc gaaagctcgc gtttttagaa
     4561 ggatacgagt tttcgctact tgtttttgat aaggtaatta tatcatggct attaaaaata
     4621 ctaaagctag aaattttgga tttttattat atcctgactc aattcctaat gattggaaag
     4681 aaaaattaga gagtttgggc gtatctatgg ctgtcagtcc tttacacgat atggacgaaa
     4741 aaaaagataa agatacatgg aataatagta atattataca aaatggaaag cactataaaa
     4801 aaccacacta tcacgttata tatattgcac gaaatcctgt aacaatagaa agcgttagga
     4861 acaagattaa gcgaaaattg gggaatagtt cagttgctca tgttgagata cttgattata
     4921 tcaaaggttc atatgaatat ttgactcatg aatcaaagga cgctattgct aagaataaac
     4981 atatatacga caaaaaagat attttgaaca ttaatgattt tgatattgac cgctatataa
     5041 cacttgatga aagccaaaaa agagaattga agaatttact tttagatata gtggatgact
     5101 ataatttggt aaatacaaaa gatttaatgg cttttattcg ccttagggga gcggagtttg
     5161 gaattttaaa tacgaatgat gtaaaagata ttgtttcaac aaactctagc gcctttagat
     5221 tatggtttga gggcaattat cagtgtggat atagagcaag ttatgcaaag gttcttgatg
     5281 ctgaaacggg ggaaataaaa tgacaaacaa agaaaaagag ttatttgctg aaaatgagga
     5341 attaaaaaaa gaaattaagg acttaaaaga gcgtattgaa agatacagag aaatggaagt
     5401 tgaattaagt acaacaatag a
//
