LOCUS       sequence-454342-       11681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 
            3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, 
            Palm W
  TITLE     Direct control of lysosomal catabolic activity by mTORC1 through 
            regulation of V-ATPase assembly.
  JOURNAL   Nat Commun. 2022 Aug 17;13(1):4848. doi: 10.1038/s41467-022-32515-6.
  PUBMED    35977928
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-17"; volume: "13(1):4848. doi"; pages: " 
            10.1038/s41467-022-32515-6"
COMMENT     Sequence Label: pLV_3xFLAG-ATP6V0A3
FEATURES             Location/Qualifiers
     source          1..11681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             125..1150
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             125..1150
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    1174..1762
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1227..1247)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1645..1656)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1969..2602
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2730..2746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2730..2746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2743..2765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2754..2770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2778..2784,2785..2802,2803..2808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2823..2844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2961..2978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3132..3720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3212..3231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3891..4682,4683..4751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4514..4533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4752..4856)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4904..5038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4941..4960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4995..5014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5206..5839
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5886..6011
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6508..6741
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6926..6970
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7233..7348
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7426..7637
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          7638..8576
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8533..8553
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8590..8655
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      10087..10096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(11254..11681,1..122)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11398..11415)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11625..11644
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
       61 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
      121 taccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
      181 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
      241 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
      301 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
      361 cattggggaa tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
      421 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
      481 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
      541 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
      601 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
      661 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
      721 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
      781 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
      841 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
      901 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
      961 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     1021 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     1081 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     1141 aaaggaatag cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt
     1201 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1261 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1321 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     1381 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     1441 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1501 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     1561 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     1621 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     1681 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     1741 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     1801 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     1861 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     1921 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     1981 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     2041 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     2101 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     2161 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     2221 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     2281 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     2341 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     2401 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2461 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2521 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2581 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     2641 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     2701 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2761 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2821 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2881 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4861 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     4921 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4981 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     5041 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     5101 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     5161 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     5221 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5281 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     5341 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     5401 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     5461 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     5521 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     5581 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     5641 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5701 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5761 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5821 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     5881 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     5941 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6001 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6061 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6121 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     6181 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     6241 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     6301 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     6361 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     6421 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     6481 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     6541 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     6601 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     6661 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     6721 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     6781 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     6841 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     6901 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     6961 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     7021 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     7081 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     7141 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     7201 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
     7261 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     7321 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     7381 tttatcgatg aggccctttc gtcttcactc gaggtgcccg tcagtgggca gagcgcacat
     7441 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     7501 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     7561 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     7621 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     7681 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     7741 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     7801 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     7861 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     7921 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     7981 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     8041 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     8101 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     8161 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     8221 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     8281 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     8341 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     8401 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     8461 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     8521 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     8581 cgtgagatgg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag
     8641 gatgacgatg acaagggctc catgttccgg agcgaggagg tggccctggt ccagctcttt
     8701 ctgcccacag cggctgccta cacctgcgtg agtcggctgg gcgagctggg cctcgtggag
     8761 ttcagagacc tcaacgcctc ggtgagcgcc ttccagagac gctttgtggt tgatgttcgg
     8821 cgctgtgagg agctggagaa gaccttcacc ttcctgcagg aggaggtgcg gcgggctggg
     8881 ctggtcctgc ccccgccaaa ggggaggctg ccggcacccc caccccggga cctgctgcgc
     8941 atccaggagg agacggagcg cctggcccag gagctgcggg atgtgcgggg caaccagcag
     9001 gccctgcggg cccagctgca ccagctgcag ctccacgccg ccgtgctacg ccagggccat
     9061 gaacctcagc tggcagccgc ccacacagat ggggcctcag agaggacgcc cctgctccag
     9121 gcccccgggg ggccgcacca ggacctgagg gtcaactttg tggcaggtgc cgtggagccc
     9181 cacaaggccc ctgccctaga gcgcctgctc tggagggcct gccgcggctt cctcattgcc
     9241 agcttcaggg agctggagca gccgctggag caccccgtga cgggcgagcc agccacgtgg
     9301 atgaccttcc tcatctccta ctggggtgag cagatcggac agaagatccg caagatcacg
     9361 gactgcttcc actgccacgt cttcccgttt ctgcagcagg aggaggcccg cctcggggcc
     9421 ctgcagcagc tgcaacagca gagccaggag ctgcaggagg tcctcgggga gacagagcgg
     9481 ttcctgagcc aggtgctagg ccgggtgctg cagctgctgc cgccagggca ggtgcaggtc
     9541 cacaagatga aggccgtgta cctggccctg aaccagtgca gcgtgagcac cacgcacaag
     9601 tgcctcattg ccgaggcctg gtgctctgtg cgagacctgc ccgccctgca ggaggccctg
     9661 cgggacagct cgatggagga gggagtgagt gccgtggctc accgcatccc ctgccgggac
     9721 atgcccccca cactcatccg caccaaccgc ttcacggcca gcttccaggg catcgtggat
     9781 gcctacggcg tgggccgcta ccaggaggtc aaccccgctc cctacaccat catcaccttc
     9841 cccttcctgt ttgctgtgat gttcggggat gtgggccacg ggctgctcat gttcctcttc
     9901 gccctggcca tggtccttgc ggagaaccga ccggctgtga aggccgcgca gaacgagatc
     9961 tggcagactt tcttcagggg ccgctacctg ctcctgctta tgggcctgtt ctccatctac
    10021 accggcttca tctacaacga gtgcttcagt cgcgccacca gcatcttccc ctcgggctgg
    10081 agtgtggccg ccatggccaa ccagtctggc tggagtgatg cattcctggc ccagcacacg
    10141 atgcttaccc tggatcccaa cgtcaccggt gtcttcctgg gaccctaccc ctttggcatc
    10201 gatcctattt ggagcctggc tgccaaccac ttgagcttcc tcaactcctt caagatgaag
    10261 atgtccgtca tcctgggcgt cgtgcacatg gcctttgggg tggtcctcgg agtcttcaac
    10321 cacgtgcact ttggccagag gcaccggctg ctgctggaga cgctgccgga gctcaccttc
    10381 ctgctgggac tcttcggtta cctcgtgttc ctagtcatct acaagtggct gtgtgtctgg
    10441 gctgccaggg ccgcctcggc ccccagcatc ctcatccact tcatcaacat gttcctcttc
    10501 tcccacagcc ccagcaacag gctgctctac ccccggcagg aggtggtcca ggccacgctg
    10561 gtggtcctgg ccttggccat ggtgcccatc ctgctgcttg gcacacccct gcacctgctg
    10621 caccgccacc gccgccgcct gcggaggagg cccgctgacc gacaggagga aaacaaggcc
    10681 gggttgctgg acctgcctga cgcatctgtg aatggctgga gctccgatga ggaaaaggca
    10741 gggggcctgg atgatgaaga ggaggccgag ctcgtcccct ccgaggtgct catgcaccag
    10801 gccatccaca ccatcgagtt ctgcctgggc tgcgtctcca acaccgcctc ctacctgcgc
    10861 ctgtgggccc tgagcctggc ccacgcccag ctgtccgagg ttctgtgggc catggtgatg
    10921 cgcataggcc tgggcctggg ccgggaggtg ggcgtggcgg ctgtggtgct ggtccccatc
    10981 tttgccgcct ttgccgtgat gaccgtggct atcctgctgg tgatggaggg actctcagcc
    11041 ttcctgcacg ccctgcggct gcactgggtg gaattccaga acaagttcta ctcaggcacg
    11101 ggctacaagc tgagtccctt caccttcgct gccacagatg actagcctcg agggcggccg
    11161 ctctagagtc gacgggccgc ggtaacaatt gttaactaac ttaagctagc aacggtttcc
    11221 ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
    11281 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    11341 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    11401 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    11461 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    11521 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    11581 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    11641 tattcaacaa ggggctgaag gatgcccaga aggtacccca t
//
