LOCUS       sequence-454343-       10871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA 
            and C-terminal mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, 
            Palm W
  TITLE     Direct control of lysosomal catabolic activity by mTORC1 through 
            regulation of V-ATPase assembly.
  JOURNAL   Nat Commun. 2022 Aug 17;13(1):4848. doi: 10.1038/s41467-022-32515-6.
  PUBMED    35977928
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-17"; volume: "13(1):4848. doi"; pages: " 
            10.1038/s41467-022-32515-6"
COMMENT     Sequence Label: pLV_3XHA-ATP6V1B2-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..10871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    109..658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(253..270)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     480..499
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    1113..1701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1166..1186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1584..1595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1908..2541
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2669..2685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2669..2685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2682..2704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2693..2709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2717..2723,2724..2741,2742..2747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2762..2783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2900..2917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3071..3659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3151..3170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3830..4621,4622..4690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4453..4472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4691..4795)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4843..4977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4880..4899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4934..4953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5145..5778
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5825..5950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6447..6680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6865..6909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7172..7287
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7365..7576
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          7577..8515
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8472..8492
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8529..8618
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             10164..10871
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      10416..10425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag
       61 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
      121 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
      181 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
      241 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
      301 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
      361 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
      421 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
      481 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
      541 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
      601 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
      661 atggtcatga aaacatttaa catttctcaa caagatctag aattagtaga agtagcgaca
      721 gagaagatta caatgcttta tgaggataat aaacatcatg tgggagcggc aattcgtacg
      781 aaaacaggag aaatcatttc ggcagtacat attgaagcgt atataggacg agtaactgtt
      841 tgtgcagaag ccattgcgat tggtagtgca gtttcgaatg gacaaaagga ttttgacacg
      901 attgtagctg ttagacaccc ttattctgac gaagtagata gaagtattcg agtggtaagt
      961 ccttgtggta tgtgtaggga gttgatttca gactatgcac cagattgttt tgtgttaata
     1021 gaaatgaatg gcaagttagt caaaactacg attgaagaac tcattccact caaatatacc
     1081 cgaaattaac ccgggcgggg cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     1141 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1201 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     1261 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     1321 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     1381 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     1441 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     1501 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     1561 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     1621 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     1681 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     1741 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     1801 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     1861 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     1921 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     1981 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     2041 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     2101 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     2161 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     2221 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     2281 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     2341 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     2401 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2461 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2521 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     2581 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga
     2641 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2881 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3001 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3481 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3901 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3961 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4021 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4081 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4141 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4201 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4261 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4321 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4381 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4441 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4501 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4561 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4621 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4681 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4741 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4801 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     4861 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4921 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     4981 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     5041 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     5101 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     5161 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5221 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     5281 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     5341 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     5401 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     5461 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     5521 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     5581 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5641 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5701 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5761 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     5821 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     5881 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     5941 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     6001 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     6061 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     6121 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     6181 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     6241 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     6301 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6361 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6421 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6481 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6541 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6601 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6661 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6721 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6781 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6841 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6901 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     6961 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     7021 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     7081 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     7141 cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta
     7201 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     7261 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     7321 ttatcgatga ggccctttcg tcttcactcg aggtgcccgt cagtgggcag agcgcacatc
     7381 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     7441 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     7501 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     7561 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     7621 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     7681 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     7741 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     7801 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     7861 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     7921 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     7981 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     8041 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     8101 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     8161 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     8221 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     8281 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     8341 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     8401 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     8461 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     8521 gtgagatgta cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact
     8581 atgcaggatc ctatccatat gacgttccag attacgctgc gctgcgggcg atgcggggga
     8641 ttgtcaacgg ggccgcaccc gagctacccg tgcccaccgg tgggccggcg gtgggagctc
     8701 gggagcaggc gctggcagtc agtcggaact acctctccca gcctcgcctc acatacaaga
     8761 cagtatctgg agtcaatggt ccactagtga tcttagatca tgttaagttt cccaggtatg
     8821 ctgaaattgt ccatttgacc ttaccggatg gcacaaagag aagtgggcaa gttctggaag
     8881 ttagtggttc caaggcagta gttcaggtat ttgaagggac ttcaggtata gatgctaaga
     8941 aaacgtcctg tgagtttact ggggatattc tccgaacacc ggtgtctgag gatatgcttg
     9001 gtcgggtatt caatggatcg ggaaaaccca ttgacagagg tcctgttgta ctggccgaag
     9061 acttccttga tatcatgggt cagccaatca accctcaatg tcgaatctac ccagaggaaa
     9121 tgattcagac tggcatttcg gccatcgatg ggatgaacag tattgctagg gggcagaaaa
     9181 ttcctatctt ctctgctgct gggctaccac acaatgagat tgcagctcag atctgtcgcc
     9241 aggctggttt ggtaaagaaa tccaaagatg tagtagacta cagtgaggaa aattttgcaa
     9301 ttgtatttgc tgctatgggt gtaaacatgg aaactgcccg gttcttcaaa tctgactttg
     9361 aagaaaatgg ctcaatggac aatgtctgcc tctttttgaa cttggctaat gacccaacca
     9421 ttgagcgaat tatcactcct cgcctggctc taaccacagc tgaatttctg gcgtaccaat
     9481 gtgagaaaca tgtattggtt attctaacag acatgagttc ttatgctgaa gcacttcgag
     9541 aggtttcagc agccagggaa gaggtacctg gtcgacgagg ttttccaggt tacatgtata
     9601 cagatttagc cacgatatat gaacgcgctg ggcgagtgga agggagaaac ggctcgatta
     9661 ctcaaatccc tattctaacc atgcctaatg atgatatcac tcaccccatc ccagacttga
     9721 ctggctacat tacagagggg cagatctatg tggacagaca gctgcacaac agacagattt
     9781 atccacctat caatgtgctg ccctcactat cacggttaat gaagtctgct attggagaag
     9841 ggatgaccag gaaggatcat gccgatgtat ctaaccagct atatgcgtgc tatgctattg
     9901 gaaaggatgt gcaagccatg aaagctgtcg ttggagaaga agcccttacc tcagatgatc
     9961 ttctctactt ggaatttctg cagaagtttg agaggaactt cattgctcag ggtccttacg
    10021 aaaatcgcac tgtctttgag actttggaca ttggctggca gctactccga atcttcccca
    10081 aagaaatgct gaagagaatc cctcagagca ccctcagcga attttaccct cgagactctg
    10141 caaagcatgg cggaggcgga tccgtgagca agggcgagga ggataacatg gcctctctcc
    10201 cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg
    10261 gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg
    10321 gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt
    10381 acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggat
    10441 accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct
    10501 acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg
    10561 ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga
    10621 cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg
    10681 gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta
    10741 actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga
    10801 ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc
    10861 tgtacaagtg a
//
