LOCUS       sequence-454344-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, 
            Palm W
  TITLE     Direct control of lysosomal catabolic activity by mTORC1 through 
            regulation of V-ATPase assembly.
  JOURNAL   Nat Commun. 2022 Aug 17;13(1):4848. doi: 10.1038/s41467-022-32515-6.
  PUBMED    35977928
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-17"; volume: "13(1):4848. doi"; pages: " 
            10.1038/s41467-022-32515-6"
COMMENT     Sequence Label: pLV_3XHA-ATP6V1B2
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    206..321
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(584..628)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    813..1046
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1543..1668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1715..2348
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2516..2650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2540..2559)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2594..2613
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2698..2802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2803..2871,2872..3663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3021..3040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3834..4422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4323..4342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4576..4593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4710..4731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4746..4751,4752..4769,4770..4776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4784..4800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4789..4811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4808..4824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4808..4824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4952..5585
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5792..6380
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5898..5909
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6307..6327
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     misc_feature    6835..7384
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6994..7013)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7223..7240
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(9026..9115)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     intron          9129..10067
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9152..9172)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(join(10068..10151,1..128))
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
       61 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
      121 ctctgcccac tgacgggcac ctcgagtgaa gacgaaaggg cctcatcgat aaactggatc
      181 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
      241 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
      301 aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat cgaatggatc
      361 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
      421 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
      481 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
      541 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
      601 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
      661 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
      721 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
      781 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
      841 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
      901 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
      961 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     1021 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     1081 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     1141 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     1201 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     1261 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     1321 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     1381 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     1441 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     1501 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     1561 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     1621 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     1681 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     1741 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     1801 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     1861 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta
     1921 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa
     1981 gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg
     2041 atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac
     2101 ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc
     2161 tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc
     2221 aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta
     2281 gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag
     2341 cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa
     2401 ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa
     2461 tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc
     2521 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2581 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2641 taaacaagtt gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc
     2701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2821 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2881 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2941 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3001 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3061 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4621 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4681 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4741 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4801 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt
     4861 aaacgctagc aatgtcaagg cctctcactc tctgatattc atttctttgc aagttataaa
     4921 tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag
     4981 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     5041 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     5101 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     5161 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc
     5221 cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg
     5281 cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc
     5341 tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc
     5401 tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa
     5461 ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc
     5521 ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc
     5581 ttccagtccc ctcttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     5641 tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc
     5701 tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt
     5761 ttctaggtct cgactgcaga attaattcca ggcggggagg cggcccaaag ggagatccga
     5821 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     5881 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     5941 atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc
     6001 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     6061 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     6121 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     6181 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     6241 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     6301 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     6361 ttgtaatcca gaggttgatt gttccagacg cgccccgccc gggttaattt cgggtatatt
     6421 tgagtggaat gagttcttca atcgtagttt tgactaactt gccattcatt tctattaaca
     6481 caaaacaatc tggtgcatag tctgaaatca actccctaca cataccacaa ggacttacca
     6541 ctcgaatact tctatctact tcgtcagaat aagggtgtct aacagctaca atcgtgtcaa
     6601 aatccttttg tccattcgaa actgcactac caatcgcaat ggcttctgca caaacagtta
     6661 ctcgtcctat atacgcttca atatgtactg ccgaaatgat ttctcctgtt ttcgtacgaa
     6721 ttgccgctcc cacatgatgt ttattatcct cataaagcat tgtaatcttc tctgtcgcta
     6781 cttctactaa ttctagatct tgttgagaaa tgttaaatgt tttcatgacc atggtattat
     6841 cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac
     6901 ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg
     6961 ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact
     7021 ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta
     7081 tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc
     7141 ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc
     7201 ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg
     7261 ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg
     7321 gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta
     7381 acgttagggg gggggggcgg aattgatccc gctagaggga aaccgttgct agcttaagtt
     7441 agttaacaat tgttaccgcg gcccgtcgac tctagagcgg ccgccctcga ggctaatgct
     7501 ttgcagagtc tcgagggtaa aattcgctga gggtgctctg agggattctc ttcagcattt
     7561 ctttggggaa gattcggagt agctgccagc caatgtccaa agtctcaaag acagtgcgat
     7621 tttcgtaagg accctgagca atgaagttcc tctcaaactt ctgcagaaat tccaagtaga
     7681 gaagatcatc tgaggtaagg gcttcttctc caacgacagc tttcatggct tgcacatcct
     7741 ttccaatagc atagcacgca tatagctggt tagatacatc ggcatgatcc ttcctggtca
     7801 tcccttctcc aatagcagac ttcattaacc gtgatagtga gggcagcaca ttgataggtg
     7861 gataaatctg tctgttgtgc agctgtctgt ccacatagat ctgcccctct gtaatgtagc
     7921 cagtcaagtc tgggatgggg tgagtgatat catcattagg catggttaga atagggattt
     7981 gagtaatcga gccgtttctc ccttccactc gcccagcgcg ttcatatatc gtggctaaat
     8041 ctgtatacat gtaacctgga aaacctcgtc gaccaggtac ctcttccctg gctgctgaaa
     8101 cctctcgaag tgcttcagca taagaactca tgtctgttag aataaccaat acatgtttct
     8161 cacattggta cgccagaaat tcagctgtgg ttagagccag gcgaggagtg ataattcgct
     8221 caatggttgg gtcattagcc aagttcaaaa agaggcagac attgtccatt gagccatttt
     8281 cttcaaagtc agatttgaag aaccgggcag tttccatgtt tacacccata gcagcaaata
     8341 caattgcaaa attttcctca ctgtagtcta ctacatcttt ggatttcttt accaaaccag
     8401 cctggcgaca gatctgagct gcaatctcat tgtgtggtag cccagcagca gagaagatag
     8461 gaattttctg ccccctagca atactgttca tcccatcgat ggccgaaatg ccagtctgaa
     8521 tcatttcctc tgggtagatt cgacattgag ggttgattgg ctgacccatg atatcaagga
     8581 agtcttcggc cagtacaaca ggacctctgt caatgggttt tcccgatcca ttgaataccc
     8641 gaccaagcat atcctcagac accggtgttc ggagaatatc cccagtaaac tcacaggacg
     8701 ttttcttagc atctatacct gaagtccctt caaatacctg aactactgcc ttggaaccac
     8761 taacttccag aacttgccca cttctctttg tgccatccgg taaggtcaaa tggacaattt
     8821 cagcatacct gggaaactta acatgatcta agatcactag tggaccattg actccagata
     8881 ctgtcttgta tgtgaggcga ggctgggaga ggtagttccg actgactgcc agcgcctgct
     8941 cccgagctcc caccgccggc ccaccggtgg gcacgggtag ctcgggtgcg gccccgttga
     9001 caatcccccg catcgcccgc agcgcagcgt aatctggaac gtcatatgga taggatcctg
     9061 catagtccgg gacgtcatag ggatagcccg catagtcagg aacatcgtat gggtacatct
     9121 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
     9181 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
     9241 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
     9301 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
     9361 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt
     9421 gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca
     9481 gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt
     9541 gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca
     9601 gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa
     9661 catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca
     9721 gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca
     9781 ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg
     9841 tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga
     9901 ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc
     9961 tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca
    10021 taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg
    10081 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
    10141 ccaccctcgg g
//
