LOCUS       sequence-454345-       12389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 
            3xFLAG and C-terminal mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, 
            Palm W
  TITLE     Direct control of lysosomal catabolic activity by mTORC1 through 
            regulation of V-ATPase assembly.
  JOURNAL   Nat Commun. 2022 Aug 17;13(1):4848. doi: 10.1038/s41467-022-32515-6.
  PUBMED    35977928
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-17"; volume: "13(1):4848. doi"; pages: " 
            10.1038/s41467-022-32515-6"
COMMENT     Sequence Label: pLV_3xFLAG-ATP6V0A3-mScarlet
FEATURES             Location/Qualifiers
     source          1..12389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(551..762)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    840..955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(1218..1262)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2177..2302
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2349..2982
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3150..3284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3174..3193)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3228..3247
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3332..3436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3437..3505,3506..4297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3655..3674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4468..5056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4957..4976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5210..5227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5344..5365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5380..5385,5386..5403,5404..5410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5418..5434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5423..5445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5442..5458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5442..5458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             5586..6219
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6426..7014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6532..6543
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6941..6961
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7038..8063)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7038..8063)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    8066..8615
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8225..8244)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8454..8471
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(8727..9419)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(9002..9021)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9271..9289
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      10481..10490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(11922..11987)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          join(12001..12389,1..550)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(12024..12044)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc
       61 caggcaccag agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg
      121 ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg
      181 cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt
      241 gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga
      301 aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag
      361 gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct
      421 cccacccact tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag
      481 taattcaagg cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca
      541 cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
      601 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
      661 cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc
      721 cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcacctcgag
      781 tgaagacgaa agggcctcat cgataaactg gatctctgct gtccctgtaa taaacccgaa
      841 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
      901 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaggcga
      961 taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     1021 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     1081 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     1141 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     1201 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     1261 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     1321 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     1381 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     1441 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     1501 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     1561 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     1621 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     1681 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     1741 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     1801 atatctcctc ctccaggtct gaagatcagc ggccggccgc ttgctgtgcg gtggtcttac
     1861 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     1921 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     1981 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     2041 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     2101 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     2161 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     2221 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     2281 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     2341 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     2401 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     2461 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     2521 gagagaccca gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact
     2581 ccccagtccc gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc
     2641 aagctcgata tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa
     2701 atgctaggcg gctgtcaaac ctccactcta acacttctct ctccgggtca tccatcccat
     2761 gcaggctcac agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat
     2821 ctggctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgacccctg
     2881 gccctggtgt gtagttctgc taatcaggga agtagccttg tgtgtggtag atccacagat
     2941 caaggatatc ttgtcttctt tgggagtgaa ttagcccttc caactactaa gtttgtagta
     3001 catatttaac aaatacaatt tctttaaaat gaaaataatt cagaggaatc acaggtttag
     3061 agtaaatgaa accacaggta attggcagtg gtaatagggt atggggtggg aagtttggga
     3121 tgattttggt tagcttgagt tatccagttg atccagacat gataagatac attgatgagt
     3181 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3241 ctattgcttt atttgtaacc attataagct gcaataaaca agttgatctc ccgatccgtc
     3301 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3361 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3421 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3481 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3541 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3601 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3661 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3721 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3781 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3841 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3901 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3961 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4021 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4081 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4141 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4201 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4261 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4321 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4381 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5101 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5161 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5221 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5281 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5341 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5401 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5461 tgattacgcc aagctctagc tagaggtcga cggtaaacgc tagcaatgtc aaggcctctc
     5521 actctctgat attcatttct ttgcaagtta taaatactga ataataagat gacatgaact
     5581 actactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     5641 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     5701 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     5761 agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc
     5821 cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag
     5881 ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg
     5941 ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca
     6001 ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg
     6061 gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc
     6121 ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa
     6181 ggatatcttg tcttcgttgg gagtgaatta gcccttccag tcccctcttt tcttttaaaa
     6241 agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga
     6301 ctggaaaacc cacctcctcc tcctcttgtg cttctagcca ggcacaatca gcattggtag
     6361 ctgctgtatt gctacttgtg attgctccat gtttttctag gtctcgactg cagaattaat
     6421 tccaggcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
     6481 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
     6541 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
     6601 ccatggaaag gacgtcagct tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
     6661 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
     6721 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
     6781 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
     6841 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
     6901 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
     6961 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattgttcca
     7021 gacgcgcccc gcccgggcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca
     7081 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
     7141 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
     7201 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
     7261 cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag
     7321 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     7381 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     7441 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     7501 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     7561 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     7621 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     7681 ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc
     7741 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
     7801 gtcaggctct cgctaaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     7861 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     7921 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     7981 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     8041 gcggtgagtt caggcttttt catggtatta tcgtgttttt caaaggaaaa ccacgtcccc
     8101 gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca
     8161 ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt
     8221 gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg
     8281 gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag
     8341 gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg
     8401 tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc
     8461 tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg
     8521 ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca
     8581 ccggccttat tccaagcggc ttcggccagt aacgttaggg ggggggggcg gaattgatcc
     8641 cgctagaggg aaaccgttgc tagcttaagt tagttaacaa ttgttaccgc ggcccgtcga
     8701 ctctagagcg gccgccctcg aggtcacttg tacagctcgt ccatgccgcc ggtggagtgg
     8761 cggccctcgg agcgttcgta ctgttccacc acggtgtagt cctcgttgtg ggaggtgatg
     8821 tccaacttgc ggtcgacgtt gtaggcgccg ggcatctgca cgggcttctt ggccttgtag
     8881 gtggtcttga agtccgccag gtagcggccg ccgtccttca ggcgcagggc catcttaatg
     8941 tcgcccttca gcacgccgtc ctcggggtac aaccgctcgg tggacgcttc ccagcccatt
     9001 gtcttcttct gcattacggg gccgtcagga gggaagttgg tgccgcggag cttcaccttg
     9061 tagatcaggg tgccgtcctc cagggaggtg tcctgggtca cggtcacggc gccgccgtcc
     9121 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggactgctt atagtagtcg
     9181 gggatgtcgg cggggtgctt gatgaaggcc ctggagccgt acatgaactg aggggacagg
     9241 atgtcccagg agaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
     9301 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcatggag
     9361 ccctccatgt gcaccttgaa ccgcatgaac tccttgatca ctgcctcgcc cttgctcacg
     9421 gatccgcctc cgccgtcatc tgtggcagcg aaggtgaagg gactcagctt gtagcccgtg
     9481 cctgagtaga acttgttctg gaattccacc cagtgcagcc gcagggcgtg caggaaggct
     9541 gagagtccct ccatcaccag caggatagcc acggtcatca cggcaaaggc ggcaaagatg
     9601 gggaccagca ccacagccgc cacgcccacc tcccggccca ggcccaggcc tatgcgcatc
     9661 accatggccc acagaacctc ggacagctgg gcgtgggcca ggctcagggc ccacaggcgc
     9721 aggtaggagg cggtgttgga gacgcagccc aggcagaact cgatggtgtg gatggcctgg
     9781 tgcatgagca cctcggaggg gacgagctcg gcctcctctt catcatccag gccccctgcc
     9841 ttttcctcat cggagctcca gccattcaca gatgcgtcag gcaggtccag caacccggcc
     9901 ttgttttcct cctgtcggtc agcgggcctc ctccgcaggc ggcggcggtg gcggtgcagc
     9961 aggtgcaggg gtgtgccaag cagcaggatg ggcaccatgg ccaaggccag gaccaccagc
    10021 gtggcctgga ccacctcctg ccgggggtag agcagcctgt tgctggggct gtgggagaag
    10081 aggaacatgt tgatgaagtg gatgaggatg ctgggggccg aggcggccct ggcagcccag
    10141 acacacagcc acttgtagat gactaggaac acgaggtaac cgaagagtcc cagcaggaag
    10201 gtgagctccg gcagcgtctc cagcagcagc cggtgcctct ggccaaagtg cacgtggttg
    10261 aagactccga ggaccacccc aaaggccatg tgcacgacgc ccaggatgac ggacatcttc
    10321 atcttgaagg agttgaggaa gctcaagtgg ttggcagcca ggctccaaat aggatcgatg
    10381 ccaaaggggt agggtcccag gaagacaccg gtgacgttgg gatccagggt aagcatcgtg
    10441 tgctgggcca ggaatgcatc actccagcca gactggttgg ccatggcggc cacactccag
    10501 cccgagggga agatgctggt ggcgcgactg aagcactcgt tgtagatgaa gccggtgtag
    10561 atggagaaca ggcccataag caggagcagg tagcggcccc tgaagaaagt ctgccagatc
    10621 tcgttctgcg cggccttcac agccggtcgg ttctccgcaa ggaccatggc cagggcgaag
    10681 aggaacatga gcagcccgtg gcccacatcc ccgaacatca cagcaaacag gaaggggaag
    10741 gtgatgatgg tgtagggagc ggggttgacc tcctggtagc ggcccacgcc gtaggcatcc
    10801 acgatgccct ggaagctggc cgtgaagcgg ttggtgcgga tgagtgtggg gggcatgtcc
    10861 cggcagggga tgcggtgagc cacggcactc actccctcct ccatcgagct gtcccgcagg
    10921 gcctcctgca gggcgggcag gtctcgcaca gagcaccagg cctcggcaat gaggcacttg
    10981 tgcgtggtgc tcacgctgca ctggttcagg gccaggtaca cggccttcat cttgtggacc
    11041 tgcacctgcc ctggcggcag cagctgcagc acccggccta gcacctggct caggaaccgc
    11101 tctgtctccc cgaggacctc ctgcagctcc tggctctgct gttgcagctg ctgcagggcc
    11161 ccgaggcggg cctcctcctg ctgcagaaac gggaagacgt ggcagtggaa gcagtccgtg
    11221 atcttgcgga tcttctgtcc gatctgctca ccccagtagg agatgaggaa ggtcatccac
    11281 gtggctggct cgcccgtcac ggggtgctcc agcggctgct ccagctccct gaagctggca
    11341 atgaggaagc cgcggcaggc cctccagagc aggcgctcta gggcaggggc cttgtggggc
    11401 tccacggcac ctgccacaaa gttgaccctc aggtcctggt gcggcccccc gggggcctgg
    11461 agcaggggcg tcctctctga ggccccatct gtgtgggcgg ctgccagctg aggttcatgg
    11521 ccctggcgta gcacggcggc gtggagctgc agctggtgca gctgggcccg cagggcctgc
    11581 tggttgcccc gcacatcccg cagctcctgg gccaggcgct ccgtctcctc ctggatgcgc
    11641 agcaggtccc ggggtggggg tgccggcagc ctcccctttg gcgggggcag gaccagccca
    11701 gcccgccgca cctcctcctg caggaaggtg aaggtcttct ccagctcctc acagcgccga
    11761 acatcaacca caaagcgtct ctggaaggcg ctcaccgagg cgttgaggtc tctgaactcc
    11821 acgaggccca gctcgcccag ccgactcacg caggtgtagg cagccgctgt gggcagaaag
    11881 agctggacca gggccacctc ctcgctccgg aacatggagc ccttgtcatc gtcatccttg
    11941 taatcgatat catgatcttt ataatcaccg tcatggtctt tgtagtccat ctcacgacac
    12001 ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc
    12061 aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc
    12121 agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa
    12181 agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc
    12241 cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga
    12301 ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc
    12361 gggaagcggc catctttccg ctcacgcaa
//
