LOCUS       sequence-454349-       10055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral construct for mRFP-LAP2-beta expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Zhao G, Liu S, Arun S, Renda F, Khodjakov A, Pellman D
  TITLE     A tubule-sheet continuum model for the mechanism of nuclear envelope
            assembly.
  JOURNAL   Dev Cell. 2023 May 22;58(10):847-865.e10. doi: 
            10.1016/j.devcel.2023.04.003. Epub 2023 Apr 24.
  PUBMED    37098350
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.04.003"; journalName: "Dev Cell"; date: 
            "2023-05-22- 22"; volume: "58"; issue: "10"; pages: "847-865.e10"
COMMENT     Sequence Label: mRFP-LAP2-beta
FEATURES             Location/Qualifiers
     source          1..10055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1137..1370
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1442..1563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1479..1498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1533..1552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1603..1738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1758..1777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1759..1777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1787..1804)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1787..1803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1796..1818)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1945..2400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2032..2051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2242..2263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2426..2530
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2531..2599,2600..3391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2749..2768)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3562..4150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4051..4070
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4304..4321
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4438..4459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4474..4479,4480..4497,4498..4504)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4512..4528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4517..4539
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4536..4552
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4536..4552
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4571..4591
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4573..4591
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4619..4845
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4846..5026
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5073..5198
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5691..5924
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6109..6153
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6420..6536
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6559..6862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6863..7066
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7017..7037
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7063..7087
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7171..7195
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7207..7216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7213..7887
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             7213..7887
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(7343..7361)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7611..7630
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7805..7828
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(8577..8601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8632..9220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8685..8705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9103..9114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9276..9775
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9311..9334)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9708..9727
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(9793..10055,1..763)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(9793..10055,1..763)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
       61 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
      121 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
      181 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
      241 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
      301 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
      361 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
      421 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
      481 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
      541 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
      601 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
      661 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
      721 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa
      781 caagagctga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg
      841 cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct aagctcgctc
      901 ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc ttctggattg tgttggtccc
      961 cagggcacga ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc agattggaga
     1021 tcgccgcccg tgcctgccga ttgggtgcag atccgtcgag ggcccggtac ctttaagacc
     1081 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     1141 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     1201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     1381 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     1441 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1561 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     1621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1681 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1741 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     1801 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1861 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1921 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2161 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2281 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2341 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     2401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2761 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3061 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3241 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4201 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4261 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4321 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4381 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4441 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4501 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     4561 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     4621 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     4681 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     4741 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     4801 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     4861 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4921 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4981 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     5041 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     5101 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     5161 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     5221 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     5281 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     5341 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     5401 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     5461 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     5521 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5581 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5641 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5701 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5761 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5821 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5881 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5941 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     6001 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     6061 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     6121 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     6181 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     6241 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     6301 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     6361 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     6421 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     6481 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     6541 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     6601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     6661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     6721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     6781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     6841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     6901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     6961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     7021 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     7081 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     7141 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctcgcta
     7201 ccggtcgcca ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg
     7261 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     7321 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     7381 gcctgggaca tcctgtcccc tcagttccag tacggctcca aggcctacgt gaagcacccc
     7441 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     7501 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     7561 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     7621 cagaagaaga ccatgggctg ggaggcctcc accgagcgga tgtaccccga ggacggcgcc
     7681 ctgaagggcg agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgccgag
     7741 gtcaagacca cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac
     7801 atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     7861 gccgagggcc gccactccac cggcgccctg tacaagtact cagatctcga gctcaagctt
     7921 gcattaacta gggagtccac gagagggtcg agaagaactc caaggagaag ggtggaaccc
     7981 tcacagcatt ttcgtgtaga tggtgcagta atttcagaga gtactcccat agctgagact
     8041 ataaaggctt ccagcaacga ctccttagtg gccaataggt tgactggaaa tttcaagcat
     8101 gcatcttcta ttctgccaat cactgaattc tcagacataa ccagaagaac accaaagaaa
     8161 ccattgacaa gagctgaagt gggagaaaaa acagaggaaa gaagagtaga aagggatatt
     8221 ctgaaggaaa tgttcccgta tgaagcctct actccaaccg gaattagtgc tagttgccgc
     8281 agaccaatca aaggtgctgc cggccggccg ctcgagctca gtgacttcag gatggaagaa
     8341 tcgttctcat ctaagtacgt cccgaagtac gttcccttgg cagacgtcaa gtcggaaaag
     8401 acaaagaagg gacgctccgt tcccatgtgg ataaaaatgc tgctgtttgc tctcgtggcg
     8461 ggttttttgt ttttggtcta tcaagctatg gaaaccaacc aaggaaaccc cttcactaat
     8521 tttcttcaag atactaaaat atccaactga ggatccaccg gatctagata actgataccc
     8581 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct
     8641 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8701 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8761 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8821 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8881 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8941 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9001 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     9061 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9121 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9181 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct gcagatatcc
     9241 ggactagtga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag
     9301 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     9361 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     9421 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     9481 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     9541 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     9601 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     9661 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     9721 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     9781 catcccgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     9841 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     9901 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     9961 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
    10021 gtgcttgaca ttggggaatt cagcgagagc ctgac
//
