LOCUS       sequence-454378-        9957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral overexpression vector to make stable bicistronic cell 
            line for control (EGFP) screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-25"; volume: "5. pii: e19809. doi"; pages: " 
            10.7554/eLife.19809"
COMMENT     Sequence Label: pLenti-DsRed_IRES_EGFP
FEATURES             Location/Qualifiers
     source          1..9957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..358)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    543..776
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1273..1398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1445..2078
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2270..2289)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2339..2355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2339..2355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2352..2374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2363..2379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2387..2393,2394..2411,2412..2417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2432..2453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2693..2710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2864..3452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2944..2963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3623..4414,4415..4483))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4246..4265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4484..4588)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4656..4674
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4793..5026
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5101..5689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5207..5218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5616..5636
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5803..6597)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5914..5933)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6524..6543
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6618..7117)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6666..6685)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7059..7082
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7127..7146
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7127..7145
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(7166..7879,7880..7882,7883..7885))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7169..7879,7880..7882,7883..7885))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7211..7232)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7560..7579
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7819..7840
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(7886..7894,7895..7897,7898..8472)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8059..8078)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8288..8305
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(8515..9192)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(8577..8600)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(8775..8794)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8992..9012
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9044..9062
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      9189..9198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9281..9305)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9302..9505)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9331..9351)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9506..9809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(9861..9957,1..21)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtgaatt cggcgatacc
       61 gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
      121 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
      181 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
      241 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
      301 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
      361 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
      421 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
      481 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
      541 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
      601 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
      661 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
      721 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
      781 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
      841 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
      901 ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt
      961 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     1021 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     1081 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     1141 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     1201 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     1261 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     1321 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     1381 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     1441 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     1501 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     1561 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     1621 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     1681 agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct tgtagcaagc
     1741 tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt gatgaaatgc
     1801 taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca tcccatgcag
     1861 gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta ccttatctgg
     1921 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga cccctggccc
     1981 tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     2041 gatatcttgt cttctttggg agtgaattag cccttccaac tactaagttt gtagtacata
     2101 tttaacaaat acaatttctt taaaatgaaa ataattcaga ggaatcacag gtttagagta
     2161 aatgaaacca caggtaattg gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat
     2221 tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg atgagtttgg
     2281 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattc gtaatcatgt
     2341 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2401 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2461 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccacggggag ctttttgcaa
     2521 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     2581 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttgctg cattaatgaa
     2641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3301 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     4261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4621 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4681 gtcttcaaga atgatctagc cctttcctta attaacccgg gcctctcact ctctgatatt
     4741 catttctttg caagttataa atactgaata ataagatgac atgaactact actgctagag
     4801 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     4861 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     4921 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     4981 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     5041 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggatctcag
     5101 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     5161 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     5221 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
     5281 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     5341 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     5401 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     5461 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     5521 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     5581 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     5641 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta ggatctatca
     5701 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     5761 aaaacctcta caaatgtggt atggctgatt atgatcctca ggtcagaaga actcgtcaag
     5821 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     5881 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     5941 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     6001 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     6061 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     6121 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     6181 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     6241 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     6301 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     6361 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     6421 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     6481 cagccggaac acggcggcat cagagcagcc gatcgtctgt tgtgcccagt catagccgaa
     6541 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatggt
     6601 aagcttgggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac
     6661 gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg
     6721 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag
     6781 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc
     6841 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc
     6901 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt
     6961 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt
     7021 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat
     7081 gctccagact gccttgggaa aagcgcctcc cctacccggt agaatttaat acgactcact
     7141 atagggagag gatcagttat ctagattact tgtacagctc gtccatgccg agagtgatcc
     7201 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     7261 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     7321 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     7381 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     7441 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     7501 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     7561 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     7621 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     7681 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     7741 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     7801 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     7861 acagctcctc gcccttgctc accatggttg tggccatatt atcatcgtgt ttttcaaagg
     7921 aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat
     7981 gtaaagcatg tgtaccgagg ccccagatca gatcccatac aatggggtac cttctgggca
     8041 tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc
     8101 caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc
     8161 ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct
     8221 acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac
     8281 agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca
     8341 gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga aaataacata
     8401 tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
     8461 gagggagagg ggcggatccc gggcccgcgg taccgtcgac tgcagaattg gccgctacag
     8521 gaacaggtgg tggcggccct cggcgcgctc gtactgctcc acgatggtgt agtcctcgtt
     8581 gtgggaggtg atgtccagct tggagtccac gtagtagtag ccgggcagct gcacgggctt
     8641 cttggccatg tagatggact tgaactccac caggtagtgg ccgccgtcct tcagcttcag
     8701 ggccttgtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc
     8761 ctcccagccc atagtcttct tctgcattac ggggccgtcg gaggggaagt tcacgccgat
     8821 gaacttcacc ttgtagatga aggagccgtc ctgcagggag gagtcctggg tcacggtcac
     8881 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
     8941 cttcttgtag tcggggatgt cggcggggtg cttcacgtac accttggagc cgtactggaa
     9001 ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt
     9061 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
     9121 gccgttcacg gagccctcca tgcgcacctt gaagcgcatg aactccttga tgacgtcctc
     9181 ggaggaggcc atggtggcga ccggtggatc tgagtccggt agcgctaggc cgccggtgtc
     9241 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     9301 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     9361 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     9421 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     9481 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     9541 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     9601 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     9661 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     9721 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     9781 gcgggccatt taccgtaagt tatgtaacgc ggaactccac tagtccaaac tggatctctg
     9841 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     9901 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta cccccca
//
