LOCUS       sequence-454422-       11960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
REFERENCE   2  (bases 1 to 11960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Feb 25. 463(7284):1035-41."
COMMENT     Sequence Label: Tet-O-FUW-Myt1l
FEATURES             Location/Qualifiers
     source          1..11960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3603..4191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3656..3676)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4074..4085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4194..4210)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4195..4211)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4716..4896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4922..4939)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4928..5152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5198..5626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5285..5304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5495..5516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5635..5655)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5640..5969
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5820..5955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5882..5901
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6017..6064
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6083..6457
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6587..6708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6624..6643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6678..6697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6757..6773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6757..6773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6770..6792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6781..6797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6805..6811,6812..6829,6830..6835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6850..6871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6988..7005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7159..7747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7239..7258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7918..8709,8710..8778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8541..8560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8779..8883)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8958..8977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9149..9528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9530..9728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9746..9926
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9973..10098
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10591..10824
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11009..11053
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11351..11468
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(11525..11543,11544..11566,11567..11585,11586..11608,
                     11609..11627,11628..11650,11651..11669,11670..11692,
                     11693..11711,11712..11734,11735..11753,11754..11776,
                     11777..11795)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        11828..11866
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11863..11887
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattgctagc caccatggac gtggactctg aggagaagcg ccatcgcaca cggtccaaag
       61 gggttcgagt tcctgtggag ccagccatac aagagctgtt cagctgtccc actccaggct
      121 gcgacggcag tggtcacgtc agtggcaaat atgcacgaca cagaagtgta tatggttgtc
      181 ccttggctaa aaaaagaaaa acgcaagata aacagcccca agaacctgct cccaagcgaa
      241 aaccatttgc agtaaaagca gatagttcct cagtagacga atgttatgag agtgatggta
      301 ctgaagacat ggatgataag gaggaagatg atgatgagga gttctctgaa gacaatgatg
      361 agcaagggga tgatgacgac gaagatgagg tggatcggga agacgaggag gagatcgagg
      421 aggaagatga tgaagaagat gatgatgatg aagatggtga cgatgtagaa gaggaagaag
      481 aggatgatga tgaagaggag gaagaagagg aagaggaaga agaaaatgaa gaccatcaaa
      541 tgagttgtac tcgaataatg caggacacag acaaggatga taacaacaat gatgagtatg
      601 ataactatga tgaactggta gctaagtcgc tattaaatct tggcaaaatt gctgaggatg
      661 cagcataccg agccaggact gaatcagaga tgaacagcaa tacctccaat agtctggagg
      721 acgatagtga caaaaacgaa aacctcggtc ggaaaagcga actgagtcta gacttagaca
      781 gtgatgttgt tagagaaaca gtggactccc ttaagctgtt agcacaagga catggtgttg
      841 tgctatcaga gaatatcagt gacagaagtt atgctgaggg gatgtcacag caggacagta
      901 gaaatatgaa ctatgtcatg ctagggaagc ccatgaacaa tggactcatg gagaagatgg
      961 tggaggagag tgatgaggaa gtgtgtctaa gtagtctaga gtgcctgagg aaccagtgct
     1021 ttgacctggc caggaaactc agcgagacca acccacagga caggagtcag ccacccaaca
     1081 tgagtgtgcg ccaacatgtc cggcaagagg acgacttccc tgggaggacg ccagacagga
     1141 gctactcgga tatgatgaac cttatgcggc tggaggagca gctcagtccc aggtctagaa
     1201 cgttctccag ctgtgccaag gaggatgggt gtcatgagag ggatgatgac accacctcag
     1261 tgaactcaga caggtctgag gaagtgtttg acatgaccaa gggcaacctg actctgctag
     1321 agaaagccat tgccttggag acagagagag ccaaggccat gcgggagaag atggccatgg
     1381 atgctgggag aagggataac ctgagatcct atgaggacca gtctccaaga cagctggctg
     1441 gggaagacag aaaatccaaa tccagtgaca gccatgtcaa aaagccatac tatggtaaag
     1501 atccctcaag aacagaaaag agagagagca agtgtccaac ccccgggtgt gatggaaccg
     1561 gccacgtaac tgggctttac ccgcatcacc gcagtctgtc tggatgcccg cacaaagata
     1621 gggtccctcc agaaattctt gccatgcatg aaaatgttct caagtgtccc actccaggct
     1681 gcacagggcg agggcatgtg aatagcaaca ggaactcgca cagaagcctc tctggatgcc
     1741 ccattgctgc tgcagaaaaa ctggcaaagg cccaagagaa acaccagagc tgtgatgtgt
     1801 ccaaatccaa ccaggcctca gaccgagtcc tcaggccaat gtgctttgtc aaacagcttg
     1861 agattcctca gtatggctac agaaacaatg ttcccacaac cacaccacgc tccaacctgg
     1921 ccaaggagct tgagaaatac tccaagactt cgtttgagta caacagttac gacaaccata
     1981 cttatggcaa aagagccata gctcccaagg tgcaaaccag ggacatatcc cccaaaggat
     2041 atgacgatgc caagcggtac tgcaagaatg ccagccccag cagcagcacc accagcagct
     2101 atgcacctag cagcagcagc aacctcagct gtggtggtgg cagcagcgcc agtagcacgt
     2161 gtagcaagag cagctttgac tacacacatg acatggaggc cgcacacatg gcagccacag
     2221 ccattctcaa cctgtccaca cgttgtcgtg aaatgccaca gaacctgtcc accaagccac
     2281 aggacctgtg tactgcccgg aacccagaca tggaggtgga tgagaatggc accctggacc
     2341 tgagcatgaa caagcagagg cctcgagaca gctgctgccc agtcctgaca cccctggaac
     2401 ccatgtctcc gcagcagcag gccgtgatga gcagccgatg cttccagctg agcgaggggg
     2461 attgctggga cttgcctgta gactacacca aaatgaagcc tcggagggta gatgaggatg
     2521 agcccaaaga gattacccca gaagacttgg acccattcca ggaggctctg gaagaaagac
     2581 ggtatccagg ggaggtgacc atcccaagcc ccaaacccaa gtaccctcag tgcaaggaaa
     2641 gcaaaaagga cttaataact ctgtctggct gccccctggc ggacaaaagc attcgaagta
     2701 tgctggccac cagttcccaa gagctcaagt gccccacccc tggctgtgac ggttctggac
     2761 acatcactgg caattacgct tctcatcgaa gcctttctgg gtgcccgaga gcgaagaaga
     2821 gtggcatccg gatagcacag agcaaagagg acaaggaaga ccaggagcca atcaggtgtc
     2881 cggtacctgg ctgtgacggt cagggacaca tcactgggaa gtatgcatcc caccgcagcg
     2941 cctccgggtg tcccttggca gccaagaggc agaaagatgg gtaccttaat ggctcccagt
     3001 tctcctggaa gtcggtcaag acggagggca tgtcctgccc tacccccggg tgtgatgggt
     3061 caggacacgt cagtggcagc ttcctcacac accgcagctt gtcaggatgt ccaagagcca
     3121 catcagcaat gaagaaagca aagctgtctg gagaacagat gttgactatc aagcagcgag
     3181 ccagcaacgg tatagaaaat gatgaagaaa tcaagcagtt agatgaagag atcaaggagc
     3241 ttaatgagtc caattcccag atggaggctg acatgatcaa actcagaact cagatcacca
     3301 caatggagag caacctgaag acgattgagg aggagaacaa agtcattgaa cagcagaatg
     3361 agtcgctctt gcacgagttg gccaacctga gccagtccct gatccacagc ctcgccaaca
     3421 tccagctgcc tcacatggat ccaatcaatg aacaaaattt tgatgcttac gtgactactt
     3481 tgacggaaat gtatacaaat caagatcgtt atcagagtcc agaaaataaa gccctactgg
     3541 aaaatataaa gcaggctgtg agaggaattc aggtctgaca attcgatatc aagcttatcg
     3601 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3961 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4141 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4201 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     4261 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     4321 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4381 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     4441 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     4501 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     4561 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     4621 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     4681 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     4741 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4801 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4861 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     4921 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4981 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5041 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5101 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5161 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5401 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5641 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5701 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5761 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5881 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5941 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     6001 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     6061 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     6121 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     6181 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     6241 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     6301 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     6361 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     6421 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     6481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     6601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     6721 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6781 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6841 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7921 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7981 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8041 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8101 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8161 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8221 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8281 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8341 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8401 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8461 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8521 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8581 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8641 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8701 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8761 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8821 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8881 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     8941 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     9001 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     9061 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     9121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     9181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     9241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     9301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     9361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     9541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     9601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     9661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     9721 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     9781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9901 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9961 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    10021 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    10081 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    10141 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    10201 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    10261 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    10321 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    10381 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    10441 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    10501 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    10561 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    10621 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    10681 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    10741 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    10801 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    10861 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    10921 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    10981 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    11041 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    11101 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    11161 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    11221 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
    11281 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
    11341 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11401 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11461 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagat ctcgagttta
    11521 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
    11581 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
    11641 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
    11701 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
    11761 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc
    11821 gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
    11881 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
    11941 atccagcctc cgcggccccg
//
