LOCUS       sequence-454423-       12795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpCas9 PE7 prime editor with P2A-human 
            MLH1dn (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y,
            Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, 
            Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B
  TITLE     Improving prime editing with an endogenous small RNA-binding 
            protein.
  JOURNAL   Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6.
  PUBMED    38570691
REFERENCE   2  (bases 1 to 12795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Apr 3. doi: 10.1038/s41586-024-07259-6."
COMMENT     Sequence Label: pCMV-PE7-P2A-hMLH1dn
FEATURES             Location/Qualifiers
     source          1..12795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             668..688
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3467..3487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3497..3523
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3533..3589
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5854..5871
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5894..5911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5900..6124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6195..6211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6195..6211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6208..6230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6219..6235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6243..6249,6250..6267,6268..6273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6288..6309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6426..6443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6597..7185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6677..6696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7356..8147,8148..8216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7979..7998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8217..8321)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8400..8419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8591..8970
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8971..9174
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9125..9145
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9216..9235
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9216..9234
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9284..9304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9305..12795,1..610)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
       61 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
      121 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
      181 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
      241 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta
      301 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
      361 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca
      421 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
      481 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
      541 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
      601 gggaggtgac tccggcggaa gctctggtgg cagcaagcgg accgccgacg gctctgaatt
      661 cgagagccct aagaagaaaa gaaaggtgag cggaggctct agcggcggaa gcaccctgaa
      721 cattgaagac gagtatagac tgcatgaaac aagcaaggaa cccgacgtgt ccctgggctc
      781 cacctggctg tccgactttc cccaggcctg ggccgagaca ggaggaatgg gcctggccgt
      841 gcggcaggca cccctgatca tccctctgaa ggccacctct acacccgtga gcatcaagca
      901 gtaccctatg tctcaggagg ccagactggg catcaagcct cacatccaga ggctgctgga
      961 ccagggcatc ctggtgccat gccagagccc ctggaacaca ccactgctgc ccgtgaagaa
     1021 gccaggcacc aatgactata gacccgtgca ggatctgaga gaggtgaaca agagggtgga
     1081 ggatatccac cccaccgtgc ccaaccctta caatctgctg tccggcctgc ccccttctca
     1141 ccagtggtat acagtgctgg acctgaagga tgccttcttt tgtctgagac tgcaccctac
     1201 cagccagcca ctgttcgcct ttgagtggag ggaccctgag atgggcatct ctggccagct
     1261 gacctggaca cgcctgcctc agggcttcaa gaatagccca acactgttta acgaggccct
     1321 gcaccgcgac ctggcagatt tccggatcca gcacccagat ctgatcctgc tgcagtacgt
     1381 ggacgatctg ctgctggccg ccaccagcga gctggattgc cagcagggaa cacgcgccct
     1441 gctgcagacc ctgggaaacc tgggatatag ggcatccgcc aagaaggccc agatctgtca
     1501 gaagcaggtg aagtacctgg gctatctgct gaaggagggc cagagatggc tgacagaggc
     1561 caggaaggag acagtgatgg gccagccaac acccaagacc ccaagacagc tgagggagtt
     1621 cctgggcaaa gcaggatttt gcaggctgtt catcccagga ttcgcagaga tggcagcacc
     1681 tctgtaccca ctgaccaagc cgggcaccct gtttaattgg ggccctgacc agcagaaggc
     1741 ctatcaggag atcaagcagg ccctgctgac agcaccagcc ctgggcctgc cagacctgac
     1801 caagcctttc gagctgtttg tggatgagaa gcagggctac gccaagggcg tgctgaccca
     1861 gaagctggga ccatggagac ggcccgtggc ctatctgtcc aagaagctgg acccagtggc
     1921 agcaggatgg ccaccatgcc tgaggatggt ggcagcaatc gccgtgctga caaaggatgc
     1981 cggcaagctg accatgggac agccactggt catcctggca ccacacgcag tggaggccct
     2041 ggtgaagcag cctccagatc gctggctgtc taacgcccgg atgacacact accaggccct
     2101 gctgctggac accgatcgcg tgcagtttgg ccctgtggtg gccctgaatc cagccaccct
     2161 gctgcctctg ccagaggagg gcctgcagca caactgtctg gacatcctgg cagaggcaca
     2221 cggaacaagg ccagacctga ccgatcagcc cctgcctgac gccgatcaca catggtatac
     2281 cgatggaagc tccctgctgc aggagggcca gaggaaggca ggagcagcag tgaccacaga
     2341 gacagaagtg atctgggcca aggccctgcc agcaggcaca tccgcccagc gggccgagct
     2401 gatcgccctg acccaggccc tgaagatggc cgagggcaag aagctgaacg tgtacacaga
     2461 ctccagatat gccttcgcca ccgcacacat ccacggagag atctacaggc gccggggctg
     2521 gctgacctct gagggcaagg agatcaagaa caaggatgag atcctggccc tgctgaaggc
     2581 cctgtttctg cccaagcggc tgagcatcat ccactgtcct ggacaccaga agggacactc
     2641 cgccgaggca aggggcaatc ggatggccga ccaggccgcc agaaaggctg ctattactga
     2701 aactcccgac acttccactc tgctgattga aaactcctcc ccttccggag gatctagcgg
     2761 aggctcctct ggctctgaga cacctggcac aagcgagagc gcaacacctg aaagcagcgg
     2821 gggcagcagc ggggggtcaa tggctgaaaa tggtgataat gaaaagatgg ctgccctgga
     2881 ggccaaaatc tgtcatcaaa ttgagtatta ttttggcgac ttcaatttgc cacgggacaa
     2941 gtttctaaag gaacagataa aactggatga aggctgggta cctttggaga taatgataaa
     3001 attcaacagg ttgaaccgtc taacaacaga ctttaatgta attgtggaag cattgagcaa
     3061 atccaaggca gaactcatgg aaatcagtga agataaaact aaaatcagaa ggtctccaag
     3121 caaaccccta cctgaagtga ctgatgagta taaaaatgat gtaaaaaaca gatctgttta
     3181 tattaaaggc ttcccaactg atgcaactct tgatgacata aaagaatggt tagaagataa
     3241 aggtcaagta ctaaatattc agatgagaag aacattgcat aaagcattta agggatcaat
     3301 ttttgttgtg tttgatagca ttgaatctgc taagaaattt gtagagaccc ctggccagaa
     3361 gtacaaagaa acagacctgc taatactttt caaggacgat tactttgcca aaaaaaatga
     3421 atctggcggc tcaaaaagaa ccgccgacgg cagcgaattc gagtctccca agaagaagag
     3481 gaaagtcggc tctggccctg ccgctaagag agtgaagctg gacggaagcg gagctactaa
     3541 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta gcttcgttgc
     3601 tggagtcatc cggagactgg acgagacagt ggtgaacaga attgccgccg gcgaggtgat
     3661 ccagagacct gccaatgcaa taaaggagat gatcgagaac tgtctggacg ccaagtccac
     3721 aagcattcag gtgatcgtga aggagggcgg actgaagctg atccagatcc aagacaacgg
     3781 cacaggcatc agaaaggaag atctggacat cgtgtgtgaa cggttcacca catctaagct
     3841 gcagtctttt gaggatctgg cctctatcag tacctacggc ttcagaggcg aggccctggc
     3901 cagcatcagc cacgtggccc atgtgaccat caccaccaaa accgccgacg gcaaatgcgc
     3961 ttatcgcgct agctacagcg acggcaagct gaaagccccg ccaaagcctt gcgccggcaa
     4021 ccagggtaca cagataacag tggaggatct gttctacaac atcgccaccc ggagaaaggc
     4081 cctgaaaaat cccagcgagg agtacggcaa gatcctggaa gtcgtcggca gatactccgt
     4141 gcacaacgcc ggaatcagct ttagcgtaaa gaagcaggga gaaaccgtgg ccgatgtgcg
     4201 caccctgcca aatgccagca ccgtggataa catcagaagc attttcggaa atgccgtgtc
     4261 cagagaactg atcgagatcg gctgcgaaga taagaccctg gcttttaaga tgaacggcta
     4321 catcagcaac gccaattact ctgtgaagaa gtgcatcttt cttctgttca tcaaccacag
     4381 actggtggaa agcaccagcc tgcggaaagc catcgagaca gtgtacgccg cctacctgcc
     4441 taagaacacc caccccttcc tgtacctgag cctcgagatc agccctcaga acgtggacgt
     4501 caatgtgcat cctacaaagc acgaggtgca cttcctgcac gaggaaagca tcctggaaag
     4561 agtgcagcag cacattgaga gcaagctgct gggctctaac agcagcagaa tgtacttcac
     4621 acagaccctg ctgcctggcc tggccggccc ctcaggcgaa atggttaagt ccacaacctc
     4681 tctgacctca tccagcacca gcggttcttc cgataaggtg tacgcccacc agatggtgcg
     4741 gaccgactct cgggagcaga agctggacgc ctttctgcaa cctctgagca aacctctgag
     4801 ctctcagcct caggccatcg tgaccgagga caagacagat atctcctccg gccgtgccag
     4861 acagcaggac gaagaaatgc tcgagctgcc agctcctgcc gaggtggccg ccaagaacca
     4921 gagcctggag ggagatacca caaagggcac cagcgaaatg agcgagaagc ggggccctac
     4981 ctccagcaac cccagaaaac ggcaccggga ggacagcgac gtggaaatgg tggaggacga
     5041 cagcagaaag gaaatgacag ccgcttgtac ccctagaaga agaatcatca acctgacctc
     5101 cgtgctgagc ctgcaggagg agatcaacga gcagggccac gaggtgctga gagagatgct
     5161 gcacaatcac agcttcgtgg gctgcgtgaa ccctcaatgg gccctggctc agcatcaaac
     5221 aaagctgtac ctgctgaaca ccaccaagct gagcgaagag ctgttctacc agatcctcat
     5281 ctacgacttc gccaacttcg gcgtgctacg cctgagcgag cccgcccctc tgtttgacct
     5341 ggccatgctg gctctggata gcccagaaag cggctggaca gaagaggacg gacctaaaga
     5401 ggggctggct gaatacatcg tggagttcct gaagaaaaag gccgagatgc tggccgacta
     5461 cttttctctg gaaatcgacg aggaaggcaa cctgatcggc ctgcctctgc tgatcgataa
     5521 ctacgtgcct cccctggaag gcctgcccat cttcatcctg agactggcta cagaggtgaa
     5581 ctgggacgag gaaaaggaat gcttcgagtc tctgagcaag gagtgcgcca tgttctatag
     5641 catcagaaaa cagtacatct ctgaagagag cactctgtct ggccagcaga gtgaagtgcc
     5701 cggaagcatc cccaacagct ggaagtggac cgtggaacac atcgtgtaca aggccctgcg
     5761 gagccacatt ctccctccta agcacttcac cgaggacggc aacatcctgc agctggccaa
     5821 cctgcccgac ctttataagg ttttctaacc ggtcatcatc accatcacca ttgagtttaa
     5881 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5941 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6001 aaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6061 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6121 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     6181 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6241 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     6301 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6481 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6541 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6601 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6661 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6721 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6781 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6841 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6901 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6961 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7021 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7081 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7141 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7201 ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7261 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7321 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7381 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7441 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7501 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7561 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7621 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7681 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7741 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7801 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7861 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7921 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7981 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8041 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8101 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8161 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8221 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8281 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8341 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt
     8401 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     8461 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     8521 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     8581 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     8641 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     8701 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     8761 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     8821 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8881 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8941 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     9001 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     9061 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     9121 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     9181 gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc
     9241 cgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa
     9301 agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
     9361 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     9421 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     9481 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     9541 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     9601 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     9661 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     9721 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     9781 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     9841 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     9901 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     9961 aaagctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
    10021 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
    10081 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
    10141 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
    10201 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
    10261 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
    10321 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
    10381 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
    10441 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aagagagagg acctgctgcg
    10501 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
    10561 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
    10621 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
    10681 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
    10741 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
    10801 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
    10861 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
    10921 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
    10981 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
    11041 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
    11101 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
    11161 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
    11221 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
    11281 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
    11341 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
    11401 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
    11461 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
    11521 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
    11581 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
    11641 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
    11701 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
    11761 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
    11821 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
    11881 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
    11941 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
    12001 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
    12061 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
    12121 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
    12181 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
    12241 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
    12301 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
    12361 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
    12421 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
    12481 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
    12541 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
    12601 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
    12661 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
    12721 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
    12781 gaaagagctg ctggg
//
