LOCUS       sequence-45443-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Bhakta MS, Segal DJ
  TITLE     The generation of zinc finger proteins by modular assembly.
  JOURNAL   Methods Mol Biol. 2010;649:3-30.
  PUBMED    20680825
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2010"; volume: "649"; pages: "3-30"
COMMENT     Sequence Label: pPGK-GZF1-RR
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(53..637)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQAREMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gttgggccat ggcggccaag cttctgcagg tcgactctag attagttaat taaaagttta
       61 tctcgccgtt attaaatttc cgtctcactt cctctaaggt taatgtgccg gctttaatca
      121 tttctccacc aattaaaagc tcttctacac taagaacagc tccattacaa ttagtgatat
      181 gatttaatcg tgtaagctga gctttgtagt ttcctttaaa gtgaccactc acaaataaaa
      241 acttaaattc cgttacagaa gatggataga ctttccacca ttcattaggg ttgatatgtt
      301 tgtttcgtgt ttgattttct tcgacatatc gttgcatttc tcgtgcttgg ccaattggca
      361 gattataacc tccgctataa gctttagtat ccacgatcac accgtaatca ataggagatc
      421 cgacagtata aattgctccg tccggtttcc ttgatccacc caaatgttta cctctatatc
      481 cataaacttt cataaaaaat tccattacct tcatttcaag aattctatcc tgagtggaat
      541 ttctggcaat ttcaattaat tcaatatatt catgaggcac atatttcaat ttatgacgaa
      601 gttcagattt cttctcctcc agttcacttt tgactagggc gcgggcggcc gcaccggtgt
      661 gagtacgttg gtggcgcacc aggttgctgg actggctgaa agacttgcca cattctggac
      721 atttgtatgg cttctcgcca gtatgagtgc gttgatggcg caccaggttg ccgctggtgc
      781 taaaagattt gccgcactct gggcatttat acggtttttc cgggcgccgc gag
//
