LOCUS       sequence-454446-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SICLOPPS precursor for cyclo-CHVVGVI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Yang L, Zhang J, Andon JS, Li L, Wang T
  TITLE     Rapid discovery of cyclic peptide protein aggregation inhibitors by 
            continuous selection.
  JOURNAL   Nat Chem Biol. 2025 Jan 13. doi: 10.1038/s41589-024-01823-x.
  PUBMED    39806068
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 13. doi: 10.1038/s41589-024-01823-x."
COMMENT     Sequence Label: pLY071c
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(72..617)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(152..171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(729..1811)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1773..1792
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1812..1889)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(2132..2137,2138..2153,2154..2160)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2168..2184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2173..2195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             complement(3034..3849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3034..3849)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3757..3776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3841..3860
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagat attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 gggacccttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
      841 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
      901 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
      961 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     1021 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     1081 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     1141 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     1201 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     1261 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     1321 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     1381 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     1441 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     1501 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     1561 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     1621 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     1681 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     1741 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     1801 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     1861 cgaaaggttt tgcgccattc gatggtgtcg cgcaacgcaa ttaatgtaag ttagctcact
     1921 cattaggcac aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt
     1981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat
     2041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
     2101 acggttctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg
     2161 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt
     2221 tcacgagcac ttcaccaaca ataggtgcag taaggaggaa aaaaaaatgg ttaaagttat
     2281 cggtcgtcgt tccttcggag tgcaaagaat atttgatatt ggtcttcccc aagaccataa
     2341 ttttctgcta gccaatgggg cgatcgccca caactgtcat gttgttggtg ttatttgtct
     2401 cagtttcggc accgaaattt taaccgttga gtacggccca ttgtccattg gcaaaattgt
     2461 gagtgaagaa attaattgtt ctgtgtacag tgttgatcca aaagggaaag tttacaccca
     2521 ggcgatcgcc caatggcatg accggggaga gcaggaagta ttggaatatg aattggaaga
     2581 tggttcagta atccgagcta cctctgaccg ccgcttttta accaccgatt atcgactggt
     2641 ggcgatcgaa gaaatttttg caaggcaact ggacttgttg actttagaaa aaaattaagc
     2701 aaactgaaga agctcttgac aaccatcgtc ttccctttcc attacttgac gctgggacaa
     2761 ttaaaacgac aaatcctggt gtatccgctt gggcaggtca acacagctta tactgcggga
     2821 caattggtca catataacgg caagacgtat aaatgtttgc agccccatac ctccttggca
     2881 ggatgggaac catccaacgt tcctgccttg tggcagcttc aatgaattaa cggcactgcg
     2941 aaaaaacccc gccgaagcgg ggttttttgc gaaatataat gaccctcttg ataacccaag
     3001 agggcatttt ttaatgccca tggcgtacgg atcttagaaa aactcatcga gcatcaaatg
     3061 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     3121 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     3181 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     3241 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     3301 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     3361 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     3421 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     3481 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     3541 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     3601 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     3661 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     3721 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     3781 taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat
     3841 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     3901 agagaaacct acggatcg
//
