LOCUS       sequence-45451-e        2017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Dittmer PJ, Miranda JG, Gorski JA, Palmer AE
  TITLE     Genetically encoded sensors to elucidate spatial distribution of 
            cellular zinc.
  JOURNAL   J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10.
  PUBMED    19363034
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2009-06-12"; volume: "284(24)"; pages: "16289-97. 
            Epub 2009 Apr 10"
COMMENT     Sequence Label: pcDNA-mito-ZifCY1
FEATURES             Location/Qualifiers
     source          1..2017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..178
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             200..274
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             296..370
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             392..466
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(497..499,500..502,503..1015)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(1292..1294,1295..1297,1298..2008)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttatgtccg tcctgacgcc
      121 gctgctgctg cggggcttga caggctcggc ccggcggctc ccagtgccgc gcgccaagat
      181 ccattcgttg ggagatccta tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg
      241 ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttgggag atcctatgtc
      301 cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc
      361 gcgcgccaag atccattcgt tgggagatcc tatgtccgtc ctgacgccgc tgctgctgcg
      421 gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg
      481 gaagcttggg gatcccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      541 cctggtcgag ctggacggcg acgtaaacgg ccacaggttc agcgtgtccg gcgagggcga
      601 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      661 cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta
      721 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      781 ggagcgtacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      841 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      901 caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc
      961 cgacaagcag aagaacggca tcaaggccca cttcaagatc cgccacaaca tcgaggacgg
     1021 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1081 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1141 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc cgcatgcatg aacgcccata
     1201 tgcttgccct gtcgagtcct gcgatcgccg cttttctcgc tcggatgagc ttacccgcca
     1261 tatccgcatc cacacaggcc agaaggagct catggtgagc aagggcgagg agctgttcac
     1321 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1381 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1441 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat
     1501 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1561 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1621 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1681 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1741 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1801 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1861 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     1921 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1981 cactctcggc atggacgagc tctacaagta agaattc
//
