LOCUS       sequence-45455-e        1983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Palmer AE, Jin C, Reed JC, Tsien RY
  TITLE     Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed 
            with an improved genetically encoded fluorescent sensor.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec
            7.
  PUBMED    15585581
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-12-14"; volume: "101(50)"; pages: 
            "17404-9. Epub 2004 Dec 7"
COMMENT     Sequence Label: pcDNA-D1ER
FEATURES             Location/Qualifiers
     source          1..1983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(52..54,55..567)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIE"
     CDS             join(1273..1275,1276..1278,1279..1791)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
ORIGIN
        1 atgctgctgc ccgtccccct gctgctgggc ctgctgggcg ccgccgccga cgtgagcaag
       61 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      241 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      541 cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 gtgaccgccg cccgcatgca tgaccaactg acagaagagc agattgcaaa attcaaagaa
      781 gccttctcat tattcgacaa ggatggggac ggcaccatca ccacaaagga acttggcacc
      841 gttatgaggt cgcttggaca aaacccaacg gaagcagaat tgcaggatat gatcaatgaa
      901 gtcgatgctg atggcaatgg aacgatttac tttcctgaat ttcttactat gatggctaga
      961 aaaatgaagg acacagacag cgaagagaga atccgaaaag cattccgtgt ttttgacaag
     1021 gatgggaacg gctacatcag cgctgctgaa ttacgtcacg tcatgacaaa cctcggggag
     1081 aagttaacag atgaagaagt tgatgaaatg ataagggaag cagatatcga tggtgatggc
     1141 caagtaaact atgaagagtt tgtacaaatg atgacagcaa agggggggaa gagggagtgg
     1201 gagaaaaact tcattgccgt cagcgctgcc aacgagttcg agaagatctc cagctccggg
     1261 gcactggagc tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1321 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1381 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1441 ccctggccca ccctcgtgac caccttcggc tacggcctga tgtgcttcgc ccgctacccc
     1501 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1561 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1621 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1681 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1741 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1801 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1861 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1921 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg caaggacgag
     1981 ctg
//
