LOCUS       sequence-454695-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ef1a downstream of a ubiquitous chromatin opening element drives 
            expression of GOI and IRES GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Khan KA, Qureshi AA, Xu P, Kuo HY, Schumacher TN, Michael IP, Kerbel
            RS
  TITLE     Immunological Tolerance to Luciferase and Fluorescent Proteins using
            Tol Mice Enables Development of Improved Tumor Models for 
            Investigating Immunity and Metastasis.
  JOURNAL   Cancer Res. 2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418.
  PUBMED    40228139
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418."
COMMENT     Sequence Label: pLUEF1 IRES GFP (backbone)
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    267..500
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
       61 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc gcggccgctg
      121 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
      181 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
      241 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
      301 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
      361 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
      421 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
      481 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
      541 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
      601 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
      661 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaat
//
