LOCUS       sequence-454697-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ef1a downstream of a ubiquitous chromatin opening element drives 
            expression of GOI and IRES GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Khan KA, Qureshi AA, Xu P, Kuo HY, Schumacher TN, Michael IP, Kerbel
            RS
  TITLE     Immunological Tolerance to Luciferase and Fluorescent Proteins using
            Tol Mice Enables Development of Improved Tumor Models for 
            Investigating Immunity and Metastasis.
  JOURNAL   Cancer Res. 2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418.
  PUBMED    40228139
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418."
COMMENT     Sequence Label: pLUEF1 IRES GFP (backbone)
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggtgtacag gagagttcgc tgctgaaagt ggtcccaaag gggtactagt ttttaagctc
       61 ccaactcccc ctcccccagc gtctggagga ttccacaccc tcgcaccgca ggggcgagga
      121 agtgggcgga gtccggtttt ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc
      181 tgaggagact ccggtcactg tcctcgcccc gcctccccct tccctcccct tggggaccac
      241 cgggcgccac gccgcgaacg gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag
      301 ggccccaatt cccagcgggc gcggcgcgcg gcccctcccc ccgccgggcg cgcgcccgct
      361 gccccgccct tcgtggccgc ccggcgtggg cggtgccacc cctccccccg gcggccccgc
      421 gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggaggggct
      481 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      541 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      601 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      661 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag a
//
