LOCUS       sequence-454698-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ef1a downstream of a ubiquitous chromatin opening element drives 
            expression of GOI and IRES GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Khan KA, Qureshi AA, Xu P, Kuo HY, Schumacher TN, Michael IP, Kerbel
            RS
  TITLE     Immunological Tolerance to Luciferase and Fluorescent Proteins using
            Tol Mice Enables Development of Improved Tumor Models for 
            Investigating Immunity and Metastasis.
  JOURNAL   Cancer Res. 2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418.
  PUBMED    40228139
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418."
COMMENT     Sequence Label: pLUEF1 IRES GFP (backbone)
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggcggggcga tacacggcgg cgcgaggcca ggcaccagag caggccgggg ccggccagct
       61 tgagactacc cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat
      121 gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg
      181 tgcagatctt ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt
      241 catcaaaaat tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc
      301 caccagattc gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc
      361 gaaggggctc cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg
      421 ggatcaagaa tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa
      481 cccgtaaaga ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca
      541 aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca
      601 ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac
      661 cttctctagg caccggttca attgccgacc cctcccccca act
//
