LOCUS       sequence-45472-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, 
            Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH
  TITLE     Interferon-inducible protein 16: insight into the interaction with 
            tumor suppressor p53.
  JOURNAL   Structure. 2011 Mar 9;19(3):418-29.
  PUBMED    21397192
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2011-03-9"; volume: "19(3)"; pages: "418-29"
COMMENT     Sequence Label: IFI16-(192-393)-HinA-E381A
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(758..776)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      772..819
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgca ggtaactccc agaagaaatg
      121 ttctccaaaa acgcccagtg atagtgaagg tactgagtac aacaaagcca tttgaatatg
      181 agaccccaga aatggagaaa aaaataatgt ttcatgctac agtggctaca cagacacagt
      241 tcttccatgt gaaggtttta aacaccagct tgaaggagaa attcaatgga aagaaaatca
      301 tcatcatatc agattatttg gaatatgata gtctcctaga ggtcaatgaa gaatctactg
      361 tatctgaagc tggtcctaac caaacgtttg aggttccaaa taaaatcatc aacagagcaa
      421 aggaaactct gaagattgat attcttcaca aacaagcttc aggaaatatt gtatatgggg
      481 tatttatgct acataagaaa acagtaaatc agaagaccac aatctacgaa attcaggatg
      541 atagaggaaa aatggatgta gtggggacag gacaatgtca caatatcccc tgtgaagaag
      601 gagataagct ccaacttttc tgctttcgac ttagaaaaaa gaaccagatg tcaaaactga
      661 tttcagcaat gcatagtttt atccagataa agaaaaaaac aaactaggga tccggctgct
      721 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      781 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      841 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
      901 gggtgactgt gccgagg
//
