LOCUS       sequence-45473-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, 
            Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH
  TITLE     Interferon-inducible protein 16: insight into the interaction with 
            tumor suppressor p53.
  JOURNAL   Structure. 2011 Mar 9;19(3):418-29.
  PUBMED    21397192
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2011-03-9"; volume: "19(3)"; pages: "418-29"
COMMENT     Sequence Label: IFI16-(192-393)-HinA-Y218A-Y267A
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(760..778)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      774..821
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgaatat tttgtttact ttagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg caggtaactc ccagaagaaa
      121 tgttctccaa aaacgcccag tgatagtgaa ggtactgagt acaacaaagc catttgaagc
      181 tgagacccca gaaatggaga aaaaaataat gtttcatgct acagtggcta cacagacaca
      241 gttcttccat gtgaaggttt taaacaccag cttgaaggag aaattcaatg gaaagaaaat
      301 catcatcata tcagattatt tggaagctga tagtctccta gaggtcaatg aagaatctac
      361 tgtatctgaa gctggtccta accaaacgtt tgaggttcca aataaaatca tcaacagagc
      421 aaaggaaact ctgaagattg atattcttca caaacaagct tcaggaaata ttgtatatgg
      481 ggtatttatg ctacataaga aaacagtaaa tcagaagacc acaatctacg aaattcagga
      541 tgatagagga aaaatggatg tagtggggac aggacaatgt cacaatatcc cctgtgaaga
      601 aggagataag ctccaacttt tctgctttcg acttagaaaa aagaaccaga tgtcaaaact
      661 gatttcagaa atgcatagtt ttatccagat aaagaaaaaa acaaactagg gatccggctg
      721 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      781 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      841 ccggatatcc cgcaggaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      901 cagggtgacg gtgccga
//
