LOCUS       sequence-45474-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, 
            Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH
  TITLE     Interferon-inducible protein 16: insight into the interaction with 
            tumor suppressor p53.
  JOURNAL   Structure. 2011 Mar 9;19(3):418-29.
  PUBMED    21397192
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2011-03-9"; volume: "19(3)"; pages: "418-29"
COMMENT     Sequence Label: IFI16-(192-393)-HinA-E222A-E272A
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(756..774)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      770..817
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgcagg taactcccag aagaaatgtt
      121 ctccaaaaac gcccagtgat agtgaaggta ctgagtacaa caaagccatt tgaatatgag
      181 accccagcaa tggagaaaaa aataatgttt catgctacag tggctacaca gacacagttc
      241 ttccatgtga aggttttaaa caccagcttg aaggagaaat tcaatggaaa gaaaatcatc
      301 atcatatcag attatttgga atatgatagt ctcctagcgg tcaatgaaga atctactgta
      361 tctgaagctg gtcctaacca aacgtttgag gttccaaata aaatcatcaa cagagcaaag
      421 gaaactctga agattgatat tcttcacaaa caagcttcag gaaatattgt atatggggta
      481 tttatgctac ataagaaaac agtaaatcag aagaccacaa tctacgaaat tcaggatgat
      541 agaggaaaaa tggatgtagt ggggacagga caatgtcaca atatcccctg tgaagaagga
      601 gataagctcc aacttttctg ctttcgactt agaaaaaaga accagatgtc aaaactgatt
      661 tcagaaatgc atagttttat ccagataaag aaaaaaacaa actagggatc cggctgctaa
      721 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      781 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      841 atatcccgca agaggcccgg cagtaccggc ataacccaag cctatgccta cagcatccag
      901 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
      961 gcgtac
//
