LOCUS       sequence-45475-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(498-663)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(653..671)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      667..714
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagccatat gctcacaaat aaattaaagc
      121 gtaaaaggag acctacatca ggccttcatc ctgaggattt tatcaagaaa gcagatttgg
      181 cagttcaaaa gactcctgaa atgataaatc agggaactaa ccaaacggag cagaatggtc
      241 aagtgatgaa tattactaat agtggtcatg agaataaaac aaaaggtgat tctattcaga
      301 atgagaaaaa tcctaaccca atagaatcac tcgaaaaaga atctgctttc aaaacgaaag
      361 ctgaacctat aagcagcagt ataagcaata tggaactcga attaaatatc cacaattcaa
      421 aagcacctaa aaagaatagg ctgaggagga agtcttctac caggcatatt catgcgcttg
      481 aactagtagt cagtagaaat ctaagcccac ctaattgtac tgaattgcaa attgatagtt
      541 gttctagcag tgaagagata aagaaaaaaa agtacaacca aatgccagtc aggcacagct
      601 aaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      661 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      721 ggaggaacta tatccggata tcccgcaaga ggcccggcag taccggcata accaagccta
      781 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
      841 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
      901 cgatgataag c
//
