LOCUS       sequence-45476-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(663-894)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(851..869)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      865..912
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagccatat gagcagaaac ctaccactca
      121 tggaaggtaa agaacctgca actggagcca agaagagtaa caagccaaat gaacagacaa
      181 gtaaaagaca tgacagcgat actttcccag agctgaagtt aacaaatgca cctggttctt
      241 ttactaagtg ttcaaatacc agtgaactta aagaatttgt caatcctagc cttccaagag
      301 aagaaaaaga agagaaacta gaaacagtta aagtgtctaa taatgctgaa gaccccaaag
      361 atctcatgtt aagtggagaa agggttttgc aaactgaaag atctgtagag agtagcagta
      421 tttcattggt acctggtact gattatggca ctcaggaaag tatctcgtta ctggaagtta
      481 gcactctagg gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt gcagcatttg
      541 aaaaccccaa gggactaatt catggttgtt ccaaagataa tagaaatgac acagaaggct
      601 ttaagtatcc attgggacat gaagttaacc acagtcggga aacaagcata gaaatggaag
      661 aaagtgaact tgatgctcag tatttgcaga atacattcaa ggtttcaaag cgccagtcat
      721 ttgctccgtt ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc tctgcccact
      781 ctgggtcctt aaagaaataa ggatccggct gctaacaaag cccgaaagga agctgagttg
      841 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      901 aggggttttt tgctgaaagg aggaactata t
//
