LOCUS       sequence-45478-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(758-936)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(683..701)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      697..744
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta ctttagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tgttgcaaac tgaaagatct gtagagagta
      121 gcagtatttc attggtacct ggtactgatt atggcactca ggaaagtatc tcgttactgg
      181 aagttagcac tctagggaag gcaaaaacag aaccaaataa atgtgtgagt cagtgtgcag
      241 catttgaaaa ccccaaggga ctaattcatg gttgttccaa agataataga aatgacacag
      301 aaggctttaa gtatccattg ggacatgaag ttaaccacag tcgggaaaca agcatagaaa
      361 tggaagaaag tgaacttgat gctcagtatt tgcagaatac attcaaggtt tcaaagcgcc
      421 agtcatttgc tccgttttca aatccaggaa atgcagaaga ggaatgtgca acattctctg
      481 cccactctgg gtccttaaag aaacaaagtc caaaagtcac ttttgaatgt gaacaaaagg
      541 aagaaaatca aggaaagaat gagtctaata tcaagcctgt acagacagtt aatatcactg
      601 caggctttcc ggtggttggt cagaaagatt aaggatccgg ctgctaacaa agcccgaaag
      661 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      721 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga
      781 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
      841 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
      901 actgtgataa actaccgcat taaagcttat cgat
//
