LOCUS       sequence-45479-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(936-1113)
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(694..712)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      708..755
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggata agccagttga
      121 taatgccaaa tgtagtatca aaggaggctc taggttttgt ctatcatctc agttcagagg
      181 caacgaaact ggactcatta ctccaaataa acatggactt ttacaaaacc catatcgtat
      241 accaccactt tttcccatca agtcatttgt taaaactaaa tgtaagaaaa atctgctaga
      301 ggaaaacttt gaggaacatt caatgtcacc tgaaagagaa atgggaaatg agaacattcc
      361 aagtacagtg agcacaatta gccgtaataa cattagagaa aatgttttta aagaagccag
      421 ctcaagcaat attaatgaag taggttccag tactaatgaa gtgggctcca gtattaatga
      481 aataggttcc agtgatgaaa acattcaagc agaactaggt agaaacagag ggccaaaatt
      541 gaatgctatg cttagattag gggttttgca acctgaggtc tataaacaaa gtcttcctgg
      601 aagtaattgt aagcatcctg aaataaaaaa gcaagaatat taaggatccg gctgctaaca
      661 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      721 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      781 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
      841 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
      901 ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgcactga
      961 attga
//
