LOCUS       sequence-454807-        1822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Hro ES5 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo24
FEATURES             Location/Qualifiers
     source          1..1822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..675)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     590..609
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(676..778)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     oriT            938..1047
                     /note="incP origin of transfer"
     rep_origin      1162..1550
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      1790..1817
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 acctaggagt caaaattact ccccgccctg ccactcatcg cagtactgtt gtaattcatt
       61 aagcattcta ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      121 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      181 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
      241 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
      301 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
      361 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
      421 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
      481 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
      541 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
      601 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
      661 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
      721 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcagt
      781 caattcgagg gggatcaatt ccgttttccg ctgcataacc ctgcttcggg gtcattatag
      841 cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc
      901 gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc
      961 cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg
     1021 ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag ggcaagcgga
     1081 tggctgatga aaccaagcca accaggaagg gcagcccacc tatcacggaa ttgatccggc
     1141 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
     1201 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
     1261 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
     1321 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
     1381 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
     1441 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
     1501 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggggtctc
     1561 ggagacgatt tcaagcaatt ataagaccgc gcggcggcgc ggatgcgccc gcttcccgcg
     1621 cagcgggata aaaatatttt gcatcgcccc ggttgacaag cgcccttgcg acggtctatt
     1681 gtaccgatcg ttcggttcag attgcatcat gcaagtcaac ggcgccgcat ccgcgggatt
     1741 gccgcaacat caacatcgat tggagagaaa cattacgaga cctcacactg gctcaccttc
     1801 gggtgggcct ttctgcgttt at
//
