LOCUS       sequence-454819-       10006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone for GFP coexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG -IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             112..906
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             112..906
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(166..185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     776..795
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1094..1113)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1138..1185
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1514..2102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2003..2022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2277..2580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2582..2780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2735..2755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2798..2978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3025..3150
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3647..3880
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4065..4109
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4407..4524
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4622..5001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5319..6335
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6259..6281
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6343..6362
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    6427..6976
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6571..6588)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6798..6817
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6979..6981,6982..6984,6985..7698)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6979..6981,6982..6984,6985..7695)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7024..7045)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7285..7304)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7632..7653
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7775..8363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7828..7848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8246..8257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8366..8382)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8367..8383)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8572..8805
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8877..8998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8914..8933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8968..8987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9038..9173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9193..9212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9194..9212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(9235..9593)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9275..9296)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9487..9506
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9620..9724
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(9726..10006,1..77)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9726..9917
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9749..9769)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(9934..10006,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
       61 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
      121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
      181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
      241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
      301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
      361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
      421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
      661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      781 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
      841 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      901 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
      961 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     1021 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     1081 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     1141 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     1201 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     1261 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     1321 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     1381 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1441 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1621 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2161 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     2221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2281 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2341 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2401 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2461 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2521 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2581 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2641 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2701 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2761 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     2821 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2881 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2941 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     3001 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     3061 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     3121 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     3181 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     3241 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     3301 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     3361 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     3421 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     3481 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3541 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3601 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3661 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3721 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3781 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3841 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3901 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3961 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     4021 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     4081 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     4141 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     4201 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     4261 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     4321 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     4381 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     4441 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4501 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     4561 ttagtaccgg gcccgctcta gccctagatt taaatcgatg tacgggccag atatacgcgt
     4621 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4681 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4741 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4801 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4861 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4921 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4981 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     5041 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     5101 atgggggcgg gggggggggg gggggggcgc ccgggggggg gtgggggggg gggggggggg
     5161 ggtggggggg gggggggggg gggggggggg cgggaggggg tggggcgagg cggagaggtg
     5221 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     5281 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
     5341 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     5401 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     5461 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     5521 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     5581 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     5641 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     5701 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     5761 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     5821 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     5881 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     5941 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     6001 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     6061 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     6121 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     6181 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     6241 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     6301 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     6361 tgctgtctca tcattttggc aaagaattcg ttaacctcga gcgggatcaa ttccgccccc
     6421 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6481 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6541 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6601 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6661 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6721 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6781 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6841 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6901 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6961 gaaaaacacg ataataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     7021 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     7081 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     7141 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     7201 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     7261 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     7321 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     7381 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     7441 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     7501 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     7561 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     7621 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     7681 gacgagctgt acaagtaaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt
     7741 caggtagccg gctaacgtta tcaagcttat cgataatcaa cctctggatt acaaaatttg
     7801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8161 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8281 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8341 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     8401 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8461 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8521 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8581 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8821 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9001 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9061 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9121 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9181 gtacccaatt cgccctatag tgagtcgtat tagcatgtga gcaaaaggcc agcataaatc
     9241 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     9301 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9361 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9421 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9481 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9541 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     9601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9721 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9781 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9841 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9901 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9961 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcag
//
