LOCUS       sequence-454824-        9238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YAP1 serine 94 o alanine mutants which disrupts binding to TEAD 
            transcription factors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel 
            AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks
            T, Hahn WC
  TITLE     KRAS and YAP1 Converge to Regulate EMT and Tumor Survival.
  JOURNAL   Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. 
            Epub 2014 Jun 19.
  PUBMED    24954536
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06.004"; journalName: "Cell"; date: "2014-07-3-
            3"; volume: "158"; issue: "1"; pages: "171-84"
COMMENT     Sequence Label: pLX304-YAP1(S94A)
FEATURES             Location/Qualifiers
     source          1..9238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    62..650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(115..135)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(533..544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             722..955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1033..1154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1070..1089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1124..1143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1194..1329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1349..1368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1350..1368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1378..1395)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1378..1394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1387..1409)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1536..1991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1623..1642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1833..1854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2017..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2122..2190,2191..2982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2340..2359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3153..3741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3642..3661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3895..3912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4029..4050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4065..4070,4071..4088,4089..4095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4103..4119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4108..4130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4127..4143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4127..4143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4162..4182
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4164..4182
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4210..4436
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4437..4617
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4664..4789
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5282..5515
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5700..5744
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5919..6419
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6338..6356
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6431..6826
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6886..7003
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7120..7423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7424..7627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7578..7598
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7650..7674
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9191..9215)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9217..9238,1..20)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
       61 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
      121 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      181 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
      241 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
      301 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
      361 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
      421 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
      481 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
      541 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
      601 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
      661 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
      721 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
      781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
      961 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     1021 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1141 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     1201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1261 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     1321 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     1381 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1441 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1501 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     1561 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1621 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1681 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1741 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1801 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1861 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     1921 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1981 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2341 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2641 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2821 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2941 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3001 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3061 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3781 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3841 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3901 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3961 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4021 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4081 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4141 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     4201 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     4261 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     4321 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     4381 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     4441 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4501 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4561 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     4621 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     4681 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     4741 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     4801 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     4861 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     4921 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     4981 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     5041 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     5101 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     5161 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     5221 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     5281 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     5341 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     5401 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     5461 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     5521 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     5581 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     5641 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     5701 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     5761 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     5821 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     5881 tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc
     5941 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     6001 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     6061 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     6121 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     6181 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     6241 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     6301 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     6361 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     6421 gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     6481 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     6541 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     6601 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     6661 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     6721 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     6781 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta
     6841 gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     6901 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6961 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     7021 cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat agtaatcaat
     7081 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     7141 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     7201 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     7261 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     7321 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     7381 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     7441 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     7501 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     7561 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     7621 cagagctctc tggctaactg tcgggatcaa caagtttgta caaaaaagtt ggcatggatc
     7681 ccgggcagca gccgccgcct caaccggccc cccagggcca agggcagccg ccttcgcagc
     7741 ccccgcaggg gcagggcccg ccgtccggac ccgggcaacc ggcacccgcg gcgacccagg
     7801 cggcgccgca ggcacccccc gccgggcatc agatcgtgca cgtccgcggg gactcggaga
     7861 ccgacctgga ggcgctcttc aacgccgtca tgaaccccaa gacggccaac gtgccccaga
     7921 ccgtgcccat gaggctccgg aagctgcccg acgccttctt caagccgccg gagcccaaat
     7981 cccactcccg acaggccagt actgatgcag gcactgcagg agccctgact ccacagcatg
     8041 ttcgagctca ttcctctcca gcttctctgc agttgggagc tgtttctcct gggacactga
     8101 cccccactgg agtagtctct ggcccagcag ctacacccac agctcagcat cttcgacagt
     8161 cttcttttga gatacctgat gatgtacctc tgccagcagg ttgggagatg gcaaagacat
     8221 cttctggtca gagatacttc ttaaatcaca tcgatcagac aacaacatgg caggacccca
     8281 ggaaggccat gctgtcccag atgaacgtca cagcccccac cagtccacca gtgcagcaga
     8341 atatgatgaa ctcggcttca ggtcctcttc ctgatggatg ggaacaagcc atgactcagg
     8401 atggagaaat ttactatata aaccataaga acaagaccac ctcttggcta gacccaaggc
     8461 ttgaccctcg ttttgccatg aaccagagaa tcagtcagag tgctccagtg aaacagccac
     8521 cacccctggc tccccagagc ccacagggag gcgtcatggg tggcagcaac tccaaccagc
     8581 agcaacagat gcgactgcag caactgcaga tggagaagga gaggctgcgg ctgaaacagc
     8641 aagaactgct tcggcaggca atgcggaata tcaatcccag cacagcaaat tctccaaaat
     8701 gtcaggagtt agccctgcgt agccagttac caacactgga gcaggatggt gggactcaaa
     8761 atccagtgtc ttctcccggg atgtctcagg aattgagaac aatgacgacc aatagctcag
     8821 atcctttcct taacagtggc acctatcact ctcgagatga gagtacagac agtggactaa
     8881 gcatgagcag ctacagtgtc cctcgaaccc cagatgactt cctgaacagt gtggatgaga
     8941 tggatacagg tgatactatc aaccaaagca ccctgccctc acagcagaac cgtttcccag
     9001 actaccttga agccattcct gggacaaatg tggaccttgg aacactggaa ggagatggaa
     9061 tgaacataga aggagaggag ctgatgccaa gtctgcagga agctttgagt tctgacatcc
     9121 ttaatgacat ggagtctgtt ttggctgcca ccaagctaga taaagaaagc tttcttacat
     9181 ggttatagac ccccaacttt cttgtacaaa gtggttggta agcctatccc taaccctc
//
