LOCUS       sequence-454842-       16152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16152)
  AUTHORS   Niu R, Zhou Y, Zhang Y, Mou R, Tang Z, Wang Z, Zhou G, Guo S, Yuan 
            M, Xu G
  TITLE     uORFlight: a vehicle toward uORF-mediated translational regulation 
            mechanisms in eukaryotes.
  JOURNAL   Database (Oxford). 2020 Jan 1;2020:baaa007. doi: 
            10.1093/database/baaa007.
  PUBMED    32168374
REFERENCE   2  (bases 1 to 16152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Database 
            (Oxford)."; date: "2020-01-1"; volume: "2020:baaa007. doi"; pages: "
            10.1093/database/baaa007"
COMMENT     Sequence Label: pGX-5Dual
FEATURES             Location/Qualifiers
     source          1..16152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..128
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             203..1228
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1269..1443
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1521..1545
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1970..2764
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4189..4777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4678..4697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4931..4948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4963..5103
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5029..5048)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5189..5211
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5447..5641
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5707..6780)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7209..7838)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9139..9163
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9351..9373
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9366..9382
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gap             9861..9960
                     /estimated_length=100
     promoter        10132..10177
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     promoter        10353..10455
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10486..10788
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10670..10689
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             10861..12513
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(10927..10945)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      12551..12803
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        13306..13651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             13692..14624
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             14715..14804
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      14826..15467
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(15505..15521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15505..15521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15518..15540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15529..15545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15553..15559,15560..15577,15578..15583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15598..15619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        16107..16152
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
       61 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      121 ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg
      181 cagatccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
      241 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
      301 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
      361 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
      421 gattccggaa gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg
      481 ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca
      541 accggtcgcg gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
      601 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
      661 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
      721 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
      781 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
      841 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
      901 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
      961 tccggagctt gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca
     1021 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     1081 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     1141 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     1201 cagcactcgt ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca
     1261 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
     1321 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     1381 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
     1441 atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     1501 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     1561 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     1621 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     1681 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     1741 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
     1801 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     1861 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     1921 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     1981 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     2041 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     2101 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     2161 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     2221 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     2281 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     2341 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     2401 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     2461 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     2521 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     2581 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     2641 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     2701 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     2761 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgct
     2821 gagagatccc ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt
     2881 agggttgcag ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact
     2941 tccatgccta agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag
     3001 tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa gccaacatgt tagttgaagc
     3061 atcagggcga ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg gctatggcgt
     3121 aggcctagtc cgtaggcagg acttttcaag tctcggaagg tttcttcaat ctgcattcgc
     3181 ttcgaataga tattaacaag ttgtttgggt gttcgaattt caacaggtaa gttagttgct
     3241 agaacccatg gctcctttgc cgacgctgag tagattttag gtgacgggtg gtgacaatga
     3301 gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag atttatacaa tagaatttgg
     3361 catgagattg gattgctttt agtcagcctc ttatagccta aagtctttga gtgactagat
     3421 gacatatcat gtaagttgct gataggtttc cagttttccg ctcctaggtc tgcatattgt
     3481 acttttcctc ttactcgact taaccagtac caacccagct tctcaacgga tttataccat
     3541 ggcactttaa agccagcatc actgacaatg agcggtgtgg tgttactcgg tagaatgctc
     3601 gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac attgctctga aagcgggaac
     3661 gctttctcat aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg caataccata
     3721 agtcgttttt gctcacgaat atcagaccag tcaacaagta caatgggcat cgtattgccc
     3781 gaacagataa agctagcatg ccaacggtat acagcgagtc gctctttgtg gaggtgacga
     3841 ttacctaaca atcggtcgat tcgtttgatg ttatgttttg ttctcgcttt ggttggcagg
     3901 ttacggccaa gttcggtaag agtgagagtt ttacagtcaa gtaatgcgtg gcaagccaac
     3961 gttaagctgt tgagtcgttt taagtgtaat tcggggcaga attggtaaag agagtcgtgt
     4021 aaaatatcga gttcgcacat cttgttgtct gattattgat ttttcgcgaa accatttgat
     4081 catatgacaa gatgtgtatc caccttaact taatgatttt taccaaaatc attaggggat
     4141 tcatcagcac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4201 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4261 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4321 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4381 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4441 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4501 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4561 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4621 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4681 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4741 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4801 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4861 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4921 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     4981 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     5041 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     5101 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5161 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5221 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
     5281 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
     5341 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
     5401 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
     5461 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
     5521 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
     5581 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
     5641 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
     5701 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
     5761 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
     5821 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
     5881 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
     5941 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
     6001 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
     6061 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
     6121 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
     6181 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
     6241 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
     6301 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
     6361 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
     6421 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
     6481 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
     6541 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
     6601 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
     6661 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
     6721 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
     6781 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg
     6841 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
     6901 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
     6961 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
     7021 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
     7081 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
     7141 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
     7201 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
     7261 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
     7321 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
     7381 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
     7441 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
     7501 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
     7561 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
     7621 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
     7681 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
     7741 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
     7801 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
     7861 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
     7921 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
     7981 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
     8041 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
     8101 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
     8161 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
     8221 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
     8281 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
     8341 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
     8401 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
     8461 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
     8521 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
     8581 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
     8641 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
     8701 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
     8761 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
     8821 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
     8881 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
     8941 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
     9001 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
     9061 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
     9121 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
     9181 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag
     9241 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     9301 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9361 acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcaaca tggtggagca
     9421 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat
     9481 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     9541 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
     9601 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
     9661 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     9721 ggattgatgt gatggtcaac atggtggagc acgacactct cgtctactcc aagaatatca
     9781 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     9841 gaaacctcct cggattccat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc
    10021 cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
    10081 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
    10141 ttcctctata taaggaagtt catttcattt ggagaggccg gtctagattc gacgacaaga
    10201 ccgggcccct cgactaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct
    10261 gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag
    10321 ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt
    10381 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
    10441 gctaaggaag ctaaactttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt
    10501 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
    10561 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
    10621 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
    10681 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
    10741 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaataca aatgtcaggc
    10801 tcccttatac acaggggccc ggctcttctc ctcaggtacc ttcgacgaca agaccctacc
    10861 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
    10921 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
    10981 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
    11041 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
    11101 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
    11161 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
    11221 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
    11281 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
    11341 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
    11401 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
    11461 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
    11521 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
    11581 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
    11641 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
    11701 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
    11761 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
    11821 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
    11881 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
    11941 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
    12001 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
    12061 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
    12121 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
    12181 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
    12241 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
    12301 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
    12361 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
    12421 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
    12481 aaggccaaga agggcggaaa gatcgccgtg taaagggctc ttctcctcac tcgagccctc
    12541 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
    12601 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
    12661 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
    12721 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
    12781 ggtgtcatct atgttactag atcgggaatt cggtccccag attagccttt tcaatttcag
    12841 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtctca tcaagacgat
    12901 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
    12961 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
    13021 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
    13081 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
    13141 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
    13201 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
    13261 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
    13321 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
    13381 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
    13441 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
    13501 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
    13561 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
    13621 ataaggaagt tcatttcatt tggagagaac acgggggacg agctcggtac cttcgacgac
    13681 aagaccgtac catggcttcc aaggtgtacg accccgagca acgcaaacgc atgatcactg
    13741 ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact
    13801 atgattccga gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctgcctcca
    13861 gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg
    13921 atctgatcgg aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc
    13981 actacaagta cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg
    14041 tgggccacga ctggggggct tgtctggcct ttcactactc ctacgagcac caagacaaga
    14101 tcaaggccat cgtccatgct gagagtgtcg tggacgtgat cgagtcctgg gacgagtggc
    14161 ctgacatcga ggaggatatc gccctgatca agagcgaaga gggcgagaaa atggtgcttg
    14221 agaataactt cttcgtcgag accatgctcc caagcaagat catgcggaaa ctggagcctg
    14281 aggagttcgc tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc
    14341 tctcctggcc tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg
    14401 tccgcaacta caacgcctac cttcgggcca gcgacgatct gcctaagatg ttcatcgagt
    14461 ccgaccctgg gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg
    14521 agttcgtgaa ggtgaagggc ctccacttca gccaggagga cgctccagat gaaatgggta
    14581 agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga gcaggcacgg ctcttctcct
    14641 cactcgacca gatctcgtac gcgtcccggg gcggtggctc atctggcgga ggtatggggt
    14701 taattaacat cttttaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc
    14761 cggactatgc aggatcctat ccatatgacg ttccagatta cgctgctcag tgcagctgac
    14821 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
    14881 ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt
    14941 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
    15001 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
    15061 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat
    15121 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
    15181 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
    15241 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
    15301 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
    15361 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
    15421 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc
    15481 cgtcgtttta gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
    15541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    15601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    15661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    15721 ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
    15781 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
    15841 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
    15901 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
    15961 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
    16021 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
    16081 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
    16141 catttggaga gg
//
