LOCUS       sequence-45485-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE
  TITLE     Polyubiquitin-sensor proteins reveal localization and linkage-type 
            dependence of cellular ubiquitin signaling.
  JOURNAL   Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888.
  PUBMED    22306808
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888."
COMMENT     Sequence Label: pQCXIP-Rx3(A6)-mEGFP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      241..250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(371..395)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(392..595)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(421..441)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tggtggcgac cggtggatcc ccagaaggcc ggcaactatt cagactttct gcaatggctt
       61 tcctcaagag ctcttcttcc tcctcggcag ccgccgctgc ggcagcttcc tgttcactca
      121 ttttgagagc cagagcaaac tgttcttctt cctcttctgc ggcagcagcg gcagccgcct
      181 cttgctcgct catcttcagt gcgagtgcga attgctcctc ctcggtcata tggctgccct
      241 ccatggtggc ggcgcggccg cggatcaatt cctgcaggtc cgttccaatg caccgttccc
      301 ggccgcggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc
      361 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
      421 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
      481 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
      541 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
      601 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
      661 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
      721 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
      781 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgac
//
