LOCUS       sequence-454868-        3091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPDG1CB0026
FEATURES             Location/Qualifiers
     source          1..3091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1135..1151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1135..1151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1148..1170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1323..1911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1403..1422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2044..2138)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2159..2818)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2733..2752
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2819..2921)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3008..3030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3022..3039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3023..3039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atggagggag agatgaaaaa catgatattt gtatgggcaa cagcgtttgg
       61 atctctcttc atctgccaca aaatcaccaa agttactgca ccaggtacaa gtcgcttatg
      121 cgcattcatc cctgtggttt gcatcttctt atacctcccc ctccggctta caactgttca
      181 tttcgccggc acaacctctt tcttcatctc ttggcttgca aacttcaaat tgattttgct
      241 tgctttcaat aaaggccctc tgttttcaaa tccacccctt cctttctcca aattcatcct
      301 caccgcttgt gttcccatta aaatttcatc tcatagatct gattctgaac ccaaaaaatc
      361 accgacggcg atcaaatact ccctcaagtt tatggtgttt cttctgttgc ttaaagtgta
      421 tgaatacgac gatcaatttc atcctctcat cagaatgact ctgttttgtg ttcatttata
      481 tgtggctcta gacctaggtt tagccatggt tgcatacctg gctcgatcca tcactggatt
      541 cgaactcgaa ccacagttcg atgaacctta cctggctaca tctctacaag acttttgggg
      601 aaagaggtgg aatctaatgg tcacgggtat actacaccct gccgtgtacc tacccgtccg
      661 agcaatttcc ggtaggtttc tgaccaaaaa gcgggccgca cttcctgctc ttatgttttc
      721 gtttatcgta tccgggctga tgcacgagtt gattttttat tacattgggc gattgaagcc
      781 cacatgggaa gtcacggggt tcttcgtcat tcatggcgtg ctggtgagta tggaggtggt
      841 ggtaaaaagg gcggtagatg gaagattcca tctgccagct gcggtttcca ggccgctggc
      901 gttgggtttg gtgatcgcca ccagcttctg gttattcttt ccgccgttcc tgcggtttga
      961 ttcggaagtc cgattatgtg aagaatttct cgccttcatg gaacttgtcc gtcgtggacg
     1021 gtttgttggc ccaaaggacg tcttttgccc ttactttaga cgcggttcga gagacctaag
     1081 gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata
     1141 gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc
     1201 gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag
     1261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1921 ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag
     1981 cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt tactagtgct
     2041 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     2101 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     2161 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2221 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2281 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2341 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2401 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2461 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2521 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     2581 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     2641 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2701 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2761 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2821 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2881 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tgatacctga
     2941 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
     3001 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
     3061 tggaataagg aagtgccatt ccgcctgacc t
//
