LOCUS       sequence-454870-        4366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0024
FEATURES             Location/Qualifiers
     source          1..4366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2410..2426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2410..2426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2423..2445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2598..3186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2678..2697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3319..3413)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3434..4093)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4008..4027
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4094..4196)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4283..4305
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4297..4314
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4298..4314
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaaat taaaaattgg tgaaaaaaat ggaaagctgg aaattggtga
       61 cggaaatggc gatgaatatt tgtatagtac caacaatttc gtagggagac aaacatggga
      121 gtttgatcca agtgcaggca cacaggaaga acgtgaccaa atcgaaagga ttcgagagca
      181 attcttgaac aataagaaga aactcgatat ccattgttgt ggtgacttgc tcatgcgaac
      241 ccagcttatc aaggaaagcc agattgatct tactagcgaa cttctagtga gactcaaaga
      301 tgaagaagat gttaactatg aagctgtgac aatggcggta aaaaaagcgg tcctcttgaa
      361 ccgtgcaatt caagcttggg acggtcattg gccagctgaa aatgccggtc cccttttttt
      421 tacccctcct ctgataattg ccttgtacat tagtggtaca gtggatacaa ttttaacaaa
      481 agaacaccaa aaggagatga ttcgctacat gtacatccat cagaatgaag atggaggatg
      541 gggattctat atatcgggga aaagcacgat gattggaagt gcattgaact atgtggctct
      601 aagacttctt ggagaagctt caccaactga tgatgatgac attggtgctc ttgctagagg
      661 ccataaatgg atatttgatc acggcggtgc tacatccatc ccttcatggg gcaaggttta
      721 tctcgcggtg cttggggtat atgaatggga aggctgcaat cctctgcctc ctgaattttg
      781 gctatttcct tcctttttgc cttatcatcc agcaaaaatg tggtgttact gtagaacgac
      841 atacatgccg atgtcacact tgtatggtaa aagctaccat gggcctatta ctgatcttgt
      901 tttgtcttta agaaaagaga ttcacaccct tccttaccac cagattaact ggaacaaaca
      961 acgacataat tgttgtaagt tggatctata ctaccctcat tcttccatac aagatatgtt
     1021 atgggatagt ctgcactatt tttgtgaacc acttgtcaaa aaatggccct tgaataagct
     1081 aagagagaag ggactcaaaa gagtggttga cctaatgcgt tatggatccg aagaaggacg
     1141 ttacataggc atgggttgtg ttgacaaggc tttacaaatg atgtgttttt atgccgagga
     1201 tccaaatggg attgacttca aacaccacct ggctagagtc cctgattact tgtggctggc
     1261 agaagatggt atgaagatgc aaagtttcgg tagtcagtta tgggattgca ctcttgtaac
     1321 tcaagcaatt atggccagta atatggttga tgagtatgga gattcactca aaaaagccca
     1381 cttttactta aaacagtcac agattaaaga gaaccctaaa ggcgatttca caaaaatgtg
     1441 tcgtttattc acgaaagggg catggacttt ctctgatcaa gaccatggat gggttgtatc
     1501 agattgcact gcagaagctt tgatttgttt attggcattg tcacaaatgc cacaagatat
     1561 tgcgggcgaa aaggctgaag ttgatagatt atatgatgct gtgaatgtcc tcctttacct
     1621 acaaagtcct gaaagtggtg gtttcgctat ttgggagcca ccagttccaa aaccatattt
     1681 acagatgttg aacccttcag aactttttgc agacatagtg gttgagaaag agcatgttga
     1741 atgtactgga tccataatcc aagcattgaa ttcattcaaa aatctgcacc cacgacaccg
     1801 tgagaaagaa atagaagctg ctatcgagaa aggcatacgt tttttggaaa acaaacaaca
     1861 aaatgatggt tcatggtacg gttattgggg tatttgtttt ctctatggca cattttttgt
     1921 gctgcaaggt ttagtatcat gcgggaaaac atatgaaaat agtgaaaccg ttcgaaaagc
     1981 tgtcaatttt ttgctctcaa cacagaattc agaaggtggt tggggagaga gttttgagtc
     2041 atgccctcaa gagaaattca tacctttgga aggaaataga acaaatttgg tgcaaacttc
     2101 atgggccatg cttggacttc tctatggtgg acaggttgaa agggatgtaa caccgttaca
     2161 caaggcagca aaattgctca ttaatggaca attggataat ggagattttc ctcaacagga
     2221 aataacggga gtgtacatga aaaactgcat gttacattat ccagaatata ggaacacttt
     2281 tccgttatgg gcgttaggag aataccgcaa ccgtgtttgg ttgccaaagc aacaagtcgg
     2341 ttcgagagac ctaaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca
     2401 tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc
     2461 tattccgcgc tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca
     2521 tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2761 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3001 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc
     3241 gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat cgtcccccca tacgatataa
     3301 gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     3361 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     3421 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     3481 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     3541 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     3601 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     3661 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     3721 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     3781 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     3841 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     3901 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     3961 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     4021 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     4081 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     4141 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     4201 ctcattgata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
     4261 ccaccacaag cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca
     4321 taatccgcac gcatctggaa taaggaagtg ccattccgcc tgacct
//
